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Proteomics
The proteome is the totality of all proteins present in a living organism, tissue, cell or cell compartment at a certain point in time under defined conditions. The proteome is subject to constant change, as proteins are constantly being synthesised or broken down. Proteome analysis is therefore mainly concerned with the separation of proteins, enzymes, peptides and amino acids. It also includes RNA and DNA analysis in some cases.
A range of chromatographic separation methods are available for the analysis of proteins and peptides. These include reversed-phase chromatography, hydrophilic and hydrophobic interaction chromatography (HILIC or HIC), ion exchange chromatography (IEX), size exclusion chromatography (SEC) and affinity chromatography (AFC).
Below you will find further information and catalogue material with application examples from various manufacturers for the analysis of proteins, peptides and amino acids as well as DNA, RNA and other related substances.
Technical Data
| Manufacturer | Column series | Description/Applications |
| AMT Halo Bioclass Columns | Protein 1000 Å | Columns with different modifications (C18, C4 and diphenyl) for very large proteins, mAbs, ADCs, biosimilars, fragments |
| Protein 400 Å | Columns with different modifications (C18 and C4) for protein analysis, e.g. mAbs | |
| Peptides 160 Å | Columns with different modifications (C18, CN and phenyl-hexyl) for analysing peptides and polypeptides up to 20 kDa. Other areas of application are tryptic digests or post-translational modifications (PTMs) | |
| Glycan 90 Å | Column especially for the analysis of glycans | |
| Concise separations | AminoSep (see table below) | For the analysis of amino acids with post-column derivatisation |
| RiboSep (see below panel) | Column especially for the separation of RNA molecules of different lengths. | |
| Imtakt | Amino acid (see below table) | Column especially for the analysis of amino acids with LC-MS without derivatisation. |
| Sepax | s. Applications below table | Many different columns for analysing proteins, peptides with different separation techniques |
| Shodex | s. Shodex catalogue below panel | Various columns for analysing proteins, peptides and other biomolecules, mainly by size exclusion chromatography, HILIC and mixed-mode techniques |
| Tosoh Bioscience | s. Tosoh catalogue below table | Various columns for the analysis of proteins, peptides and other biomolecules, mainly by size exclusion and ion exchange chromatography, HILIC, HIC and affinity chromatography. |
Downloads
Brochures
AMT HALO Bioclass Brochure
Shodex HPLC Columns Catalog 2023-2024
Tosoh Chromatography Catalogue 2019
Applications
Concise Separations Columns for Amino Acid Analysis
Concise Separations Columns for RNA, Protein & Peptides
Imtakt PRTM Intrada Amino Acid Application
Sepax Unix-P1002 SEC-1.8um Application Notes
Sepax Complex Protein Sample Mixtures Analysis
Sepax Fusion Protein Separation on SEC & SAX
The right column for you - we will be happy to support you individually
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Certificates of analysis, MSDS, data sheets