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Membrane Proteins

Membrane proteins are proteins that are associated with a cell membrane or membranes of cell compartments. Transmembrane proteins permeate the cell membrane completely, whereas membrane proteins are only attached to one side of the lipid bilayer.

 

Membrane proteins can be analysed using all separation techniques of protein analysis. These are reversed phase chromatography, hydrophilic and hydrophobic interaction chromatography (HILIC or HIC), ion exchange chromatography (IEX), size exclusion chromatography (SEC) and affinity chromatography (AFC)

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TSKgel Ether-5PW, 10 µm, 1.000 Å 75 mm x 7.5 mm
TSH-08641
TSKgel DEAE-5PW, 10 µm, 1.000 Å 75 mm x 7.5 mm
TSH-07164
TSKgel G3000SW, 10 µm, 250 Å 600 mm x 7.5 mm
TSH-05103
Sepax SRT-C SEC-500 5µm 500Å 300x7.8mm HPLC-Column
SPX-235500-7830
€2,159.00
Sepax SRT-C SEC-500 5µm 500Å 300x4.6mm HPLC-Column
SPX-235500-4630
€1,809.00
Sepax SRT-C SEC-300 5µm 300Å 300x7.8mm HPLC-Column
SPX-235300-7830
€2,036.00
Sepax SRT-C SEC-300 5µm 300Å 300x4.6mm HPLC-Column
SPX-235300-4630
€1,726.00
SRT SEC-500 5µm 500Å 300x7.8mm HPLC-Column
SPX-215500-7830
€2,045.00
SRT SEC-500 5µm 500Å 50x7.8mm HPLC-Column
SPX-215500-4630
€1,716.00
SRT SEC-300 5µm 300Å 300x4.6mm HPLC-Column
SPX-215300-4630
€1,533.00
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Applications

Size Exclusion Chromatography of Membrane Protein Apqz in octylglucoside with Sepax columns

Peak identities

Apqz

Test conditions

Columns: Nanofilm SEC-250 300x7.8mm

                SRT-C SEC-300 300x7.8mm

                SRT SEC-300 300x7.8mm

Mobile phase: 20 mM TrisHCl, pH 7, 190 mM NaCl, 10 mM KCl, 40 mM octyl glucoside

Flow rate: 1 mL/min

Detection: UV, 280 nm

Injection: 2 µL with 6 mg/mL

Ion Exchange of Membrane Proteins with Tosoh TSKgel DEAE-5PW

Peak identities

Membrane proteins

Test conditions

Column: TSKgel DEAE-5PW 75x7.5mm, 10µm (07164)

Mobile phase A: 20% glycerol + octaethyleneglycol dodecylether in 20 mmol/L Tris-HCl, pH 8

Mobile phase B: 1 mol/L sodium chloride + 20% glycerol + octaethyleneglycol dodecyl ether in 20 mmol/L Tris-HCl, pH 8

Gradient: 0 to 100%B in 60 min

Flow rate: 1 mL/min

Detection: UV/Vis, 417nm

Sample: Crude extract from rat liver microsome

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Manufacturer overview

Sepax

HPLC - SEC:

  • Nanofilm SEC-500, 5µm
  • Nanofilm SEC-250, 5µm
  • SRT-C SEC-500, 5µm
  • SRT-C SEC-300, 5µm
  • SRT SEC-500, 5µm
  • SRT SEC-300, 5µm

Tosoh

HPLC - HIC:

  • TSKgel Ether-5PW, 10µm

 

HPLC- IEX:

  • TSKgel DEAE-5PW, 10µm

 

HPLC - SEC:

  • TSKgel G3000SW, 10µm

For further information and application examples for analysing proteins, see Proteome analysis.

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