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Glycan
Glycans - Analysis of Oligo- and Polysaccharides, Glycoproteins and other related Substances
Glycans are polysaccharides, i.e. large organic molecules that consist of many individual glycosidically linked monosaccharide building blocks. In the literature, the term "glycan" also refers to certain carbohydrate parts of glycoconjugates, e.g. glycoproteins or glycolipids. Well-known substances that are glycans include cellulose, chitin and heparin. Carbohydrates and their conjugates are of enormous physiological importance in plants, animals and humans.
Glycans are often analysed using HPLC-(MS), whereby different separation techniques can be used depending on the analyte.
Technical Data
1. Advanced Materials Technology™
The HALO® GLYCAN core-shell columns from AMT are HPLC columns specially developed for analysing glycans. They are ideal for analysing oligosaccharides and other carbohydrate residues derived from glycoproteins and proteoglycans, for example. The functional group is a novel ligand containing 5 hydroxyl groups, which are bound to the fused-core silica particles via a special linker. Ideally, these columns are used in HILIC separation mode.
Application
Further Technical Data:
| Phase Name | Particle Size | Pore Size | Carbon Load | Surface Area | Endcapping | pH Range | Max. Temp. |
| HALO GLYCAN | 2.7 µm | 90 Å | 3.2% | 135 m2/g | No | 2-9 | 65° C* |
*40 °C at upper pH-Limit
2. Sepax Technologies Inc.™
For the separation of various glycans, glycoproteins and other substances using ion exchange chromatography, Sepax offers the Proteomix SAX HPLC columns. Glycans and related substances can also be analysed by size exclusion chromatography. Sepax offers the Zenix SEC columns for this purpose. Below you will find some application examples:
| Phase Name | Particle Size | Base Material | Pore Size | Ligand | Dynamic Binding Capacity | pH Range | Max. Temp. |
| Proteomix SAX | 1.7, 3, 5, 10 µm | PS/DVB grafted with highly hydrophilic, | Non-Porous | -N+(CH3)3 | ~34, 31, 17, 13 mg/mL | 2-12 | 80° C |
| Phase Name | Particle Size | Base Material | Pore Size | Protein (native) MW Range | pH Range | Max. Temp. | Salt Concentration Range |
| Zenix SEC-150 Zenix SEC-300 | 3 µm | Neutral, hydrophilic film bonded silica | 150 Å, 300 Å | 500-150.000 | 2-8.5 | 80° C | 20 mM-2.0 M* |
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