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DNA - RNA
DNA (deoxyribonucleic acid) and RNA (ribonucleic acid) are essential biomolecules that act as carriers of genetic information and play a central role in the biology of all living organisms. DNA stores the genetic information necessary for the development, function and reproduction of organisms, while RNA serves as a mediator in the transmission of this information and in protein synthesis. The structural differences between DNA and RNA lie in their sugar components and the bases they contain, which gives them different functions in cell metabolism.
High performance liquid chromatography (HPLC) is an analytical method commonly used to separate, identify and quantify DNA and RNA components. This technique is particularly important as it offers high precision and sensitivity, which is crucial for analysing nucleic acids in research and diagnostics. By using HPLC, researchers can efficiently analyse specific nucleotides, nucleosides and their modifications, which is essential for understanding genetic processes and developing therapeutic approaches.
Technical Data
Differences between nucleotides, oligonucleotides and DNA/RNA
The terms nucleotide, oligonucleotide and DNA/RNA refer to different molecular sizes in the analysis of nucleic acids that can be analysed by HPLC.
- Nucleotides are the basic building blocks of DNA and RNA, consisting of a base, a sugar and a phosphate group. HPLC analysis of nucleotides involves identifying and quantifying individual nucleotides. This analysis can be used, for example, to determine the content of specific nucleotides in a sample or to analyse their modifications.
- Oligonucleotides are short chains of nucleotides, typically up to about 20-30 base pairs in length. HPLC is used to check the purity, composition and sequence of these short DNA or RNA fragments. This analysis is particularly important in the production of synthetic oligonucleotides used in research or medicine.
- DNA/RNA analysis using HPLC involves analysing longer DNA or RNA strands, which can comprise several hundred to thousands of base pairs. In this analysis, structure, modifications and fragmentation patterns can be analysed. For long strands, HPLC is often used in combination with other techniques such as mass spectrometry or gel electrophoresis to analyse the complete sequence or structure.
Applications
Separation of different RNA chain lengths with Concise RiboSep RNA 50x7.8mm, 2µm
Peak identities
1. 100nt 2. 200nt 3. 300nt 4. 400nt 5. 500nt
Test conditions
Column: RiboSep RNA 50x7.8mm, 2µm
Mobile phase: A: 0.1M TEA; B: 0.1M TEAA, 25% ACN
Isocratic: 15min
Injection volume: 10µL
Separation of different DNA oligonucleotides with ADS Biotec DNASep
1. n-2 ( 5'-AAA AGT CCG TGA GA-3' ; 16.4 min)
2. n-1 ( 5'-CAA AAG TCC GTG AGA-3' ; 17.1 min)
3. 16mer (5'-ACA AAA GTC CGT GAG A-3' ; 17.8 min)
Downloads
ADS Biotec
DNA - Reversed Phase:
- DNASep
RNA - Reversed Phase:
- RNASep
Nucleic acids - reversed phase:
- OligoSep
- ADS Biotec DNASep Quality Control and Ourification of Oligonucleotides Applications
- ADS Biotec DNASep Sizing of Fragments with the WAVE Nucleic Acid Fragment Analysis System Applications
- ADS Biotec RNASep RNA Chromatography Under Thermally Denaturing Conditions: Analysis and Quality Determination of RNA Applications
- ADS Biotec RNASep RNA Chromatography Under Thermally Denaturing Conditions: Quantitation of RNA Applications
- ADS Biotec RNASep Brochure
- ADS Biotec OligoSep Purification of Duplex siRNAs Directly from Crude, Annealed Oligonucleotides Applications
Concise
DNA/RNA - Reversed Phase:
- RiboSep, 2µm
Imtakt
siRNA - Reversed Phase:
- Scherzo SW-C18
DNA - Reversed Phase:
- Presto FF-C18
- Cadenza CW-C18
- Cadenza CL-C18
DNA - SEC:
- Intrada SEC
Macherey-Nagel
DNA fragments - IEX:
- Nucleogenic DEAE, 7µm
RNA - IEX:
- Nucleogen DEAE, 7µm
tRNA, rRNA, DNA - IEX:
- Nucleogen DEAE, 7µm
- Macherey-Nagel Nucleogen DEAE Separation of Crude RNA Extract of Viroid (PSTV) infected Tomato Plants
- Macherey-Nagel Nucleogen DEAE Restriction Fragments from Plasmid pBR 322 cleaved with HinfI
- Macherey-Nagel Nucleogen DEAE DNA Restriction Fragments from Plasmid pBR 322 cleaved with HaeIII
- Macherey-Nagel Nucleogen DEAE Separation of DNA Restriction Fragments
- Macherey-Nagel Nucleogen DEAE Elution of Recombinant Yeast Cell Lysate
Nacalai Tesque
DNA - IEX:
- Cosmogel IEX Type Q-N
DNA - SEC:
- Cosmosil Diol-120-II
- Cosmosil Diol-300-II
- Cosmosil Diol-1000-II
- Cosmosil Diol-2000-II
- Cosmosil RNA-SEC-1000
- Cosmosil RNA-SEC-2000
DNA - Reversed Phase:
- Cosmosil RNA-RP1
DNA Digest - IEX:
- Cosmogel IEX Type Q-N
CNT-DNA - HILIC:
- Cosmosil CNT-300
- Cosmosil CNT-1000
- Cosmosil CNT-2000
dsRNA, mRNA, ssRNA - Reversed Phase:
- Cosmosil RNA-RP1
dsRNA, ssRNA - SEC:
- Cosmosil RNA-SEC-1000
- Cosmosil RNA-SEC-2000
- Nacalai Tesque Cosmogel OEX Type Q-N 100bp DNA Ladder
- Nacalai Tesque Cosmogel IEX Type Q-N DNA Hind III Digest
- Nacalai Tesque Cosmogel IEX Type Q-N DNA Hind III Digest
- Nacalai Tesque Cosmogel IEX Typer Q-N DNA pd(A)40-60
- Nacalai Tesque Cosmosil CNT DNA-wrapped CNT
- Nacalai Tesque Cosmosil 5Diol 100bp DNA Ladder
- Nacalai Tesque Cosmosil 5Diol dsDNA
- Nacalai Tesque Cosmosil 5Diol dsDNA
- Nacalai Tesque Cosmosil RNA-RP1 20bp DNA Ladder
- Nacalai Tesque Cosmosil RNA-RP1 100bp DNA Ladder
- Nacalai Tesque Cosmosil RNA-RP1 dsRNA Ladder
- Nacalai Tesque Cosmosil RNA-RP1 mRNA
- Nacalai Tesque Cosmosil RNA-RP1 ssRNA Ladder
- Nacalai Tesque Cosmosil RNA-SEC-1000 ssRNA Ladder, dsRNA LAdder
- Nacalai Tesque Cosmosil RNA-SEC-2000 ssRNA Ladder, dsRNA Ladder
- Nacalai Tesque Cosmosil RNA-SEC 20bp DNA Ladder
- Nacalai Tesque Cosmosil RNA-SEC 100bp DNA Ladder
- Nacalai Tesque Cosmosil RNA-SEC dsRNA Ladder
- Nacalai Tesque Cosmosil RNA-SEC ssRNA Ladder Low Range
- Nacalai Tesque Cosmosil RNA-SEC ssRNA Ladder
Sepax
DNA - Reversed Phase:
- Proteomix RP-300, 10µm
DNA - IEX:
- Proteomix WAX, 5µm
DNA - SEC:
- Zenix SEC-300, 3µm
RNA - IEX:
- Proteomix WAX, 5µm
RNA - SEC:
- SRT SEC-2000, 5µm
mRNA - Reversed Phase:
- Proteomix RP-1000, 5µm
mRNA - SEC:
- Zenix SEC-300, 3µm
- SRT SEC-1000, 5µm
mRNA - affinity chromatography:
- Monomix dT(20), 30µm
circular RNA - Reversed Phase:
- Bio-C18, 5µm
circular RNA - SEC:
- SRT SEC-2000, 5µm
circular mRNA - SEC:
- SRT SEC-1000, 5µm
- Sepax Proteomix RP-300 Separation of DNA
- Sepax Proteomix WAX Analysis of DNA
- Sepax Zenix SEC-300, Proteomix SAX DNA Separation
- Sepax mRNA HPLC Analysis on SEC, RP, Affinity and IEX
- Sepax Proteomix WAX Analysis of RNA
- Sepax SRT SEC-1000 Circular mRNA Purification
- Sepax SRT SEC-1000, Proteomix RP-1000 mRNA Separation
- Sepax SRT SEC-2000, Bio-C18 Circular RNA Separation
- Sepax SRT SEC-2000 Separation of High Molecular Weight RNA
- Sepax Zenix SEC-300 mRNA-LNP (Lipid Nanoparticle)
Shimadzu
mRNA - Reversed Phase:
- Shim-pack Scepter Claris C18, 1.9µm
Shodex
tRNA - IEX:
- IEC DEAE-825
Thermo
mRNA - Reversed Phase:
- DNAPac RP, 4µm
mRNA - IEX:
- DNAPac PA200 RS, 4µm
Tosoh
DNA - Reversed Phase:
- TSKgel OligoDNA RP, 5µm
DNA - IEX:
- TSKgel DNA-NPR, 2.5µm
- TSKgel DNA-STAT, 5µm
DNA - SEC:
- TSKgel G6000PWXL, 13µm
- TSKgel G5000PWXL, 10µm
- TSKgel G4000PWXL, 10µm
- TSKgel GMPWXL, 13µm
tRNA - IEX:
- TSKgel DEAE-NPR, 2.5µm
rRNA - IEX:
- TSKgel DEAE-NPR, 2.5µm
dsRNA - IEX:
- TSKgel DNA-STAT, 5µm
- Tosoh TSKgel DNA-NPR Separation of DNA Fragments
- Tosoh TSKgel DNA-NPR Effect of pH in Eluent on Separation of 1kb DNA Ladder
- Tosoh TSKgel DNA-NPR Separation of PCR Product
- Tosoh TSKgel DNA-NPR Separation of PCR Product Interleukin-1
- Tosoh TSKgel DNA-STAT Separation of 1kb DNA Ladder Marker
- Tosoh TSKgel DNA-STAT Separation of 1kb DNA Ladder MArker NaCL and NaClO4 Gradient
- Tosoh TSKgel DNA-STAT Separation of DNA Marker (pBR322-HaeIII Digest)
- Tosoh TSKgel DNA-STAT Separation of DNA Marker (pBR322-HaeIII Digest) NaCl and NaClO4 Gradient
- Tosoh TSKgel DNA-STAT Separation of DNA Marker (Phi X174-HaeIII Digest)
- Tosoh TSKgel DNA-STAT Separation of DNA Marker (Phi X174 -HaeIII Digest) NaCl and NaClO4 Gradient
- Tosoh TSKgel DNA-STAT Separation of dsRNA
- Tosoh TSKgel DNA-STAT Separation of dsRNA NaCl and NaClO4 Gradient
- Tosoh TSKgel G6000PWXL, G5000PWXL, G4000PWXL DNA-Na
- Tosoh TSKgel GMPWXL DNA-Na
- Tosoh TSKgel OligoDNA RP Separation of DNA from Clupeoid Seminal Fluid
- Tosoh TSKgel DEAE-NPR Separation of 16S, 23S rRNA from E. Coli R-13
- Tosoh TSKgel DEAE-NPR Separation of tRNA from Baker´s Yeast
- Tosoh TSKgel DEAE-NPR Separation of tRNA from E. Coli
Waters
DNA - Reversed Phase:
- ACQUITY Peptide Separation Technology BEH300 C18, 1.7µm
DNA - IEX:
- Protein-Pak Hi Res Q, 5µm
dsDNA - IEX:
- Protein-Pak Hi Res Q, 5µm
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