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Heparin
Heparin refers to a group of the body's own unbranched glucosaminoglycans (polysaccharides) that have an inhibitory effect on the blood coagulation cascade. The monomer units of these polysaccharides are glucosamine, glucuronic acid and iduronic acid. In many cases, they carry sulphate groups on the oxygen and nitrogen atoms of the side chains. The molecular weight of heparin is approximately between 4000 and 50000 g/mol. The figure opposite shows a structural section of a heparin molecule.
Due to the many functional groups present in the molecule, in particular the negatively charged sulphate or carboxylate groups, heparin is a very polar molecule which exhibits good solubility properties in water. Suitable separation techniques therefore include ion exchange chromatography and gel filtration chromatography (SEC with aqueous eluents). Below you will find some application examples.
Applications
Size Exclusion Chromatography of Enoxaparin with Sepax Zenix SEC
Peak identities
Enoxaparin Sodium
Test conditions
Column: Zenix SEC-100 300x7.8mm, 3µm
Zenix SEC-150 300x7.8mm, 3µm
Zenix SEC-300 300x7.8mm, 3µm
Ion Exchange Chromatography of Heparin with Sepax Glycomix SAX
Peak identities
1. DS (Dermatan Sulfate) 2. heparin 3. OSCS (Oversulfated Chondroitin Sulfate)
Test conditions
Column: Glycomix SAX 250x4.6mm (901665-4625)
Downloads
Manufacturer overview
Imtakt
UHPLC - Reversed Phase:
- Presto FF-C18, 2µm
Sepax
HPLC - SEC:
- SRT SEC-300, 5µm
- SRT SEC-500, 5µm
- Zenix SEC-100, 3µm
- Zenix SEC-150, 3µm
- Zenix SEC-300, 3µm
HPLC - IEX:
- Glycomix SAX
Shodex
HPLC - SEC:
- OHpak SB-804 HQ, 10µm
- OHpak SB-806M HQ, 13µm
- OHpak LB-806M, 13µm
Thermo Fisher
HPLC - Ion chromatography:
- Dionex IonPac AS11-HC, 4µm
- Dionex CarboPac PA20, 6.5µm
Tosoh
HPLC - SEC
- TSKgel G2000SWXL, 5µm
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