Antibodies mAb

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Antibodies mAB

Monoclonal antibodies (mAb's) are immunologically active proteins that have increasingly become the focus of medical research in recent years. Today, a large number of modern pharmaceuticals and therapeutic approaches based on monoclonal antibodies already exist

The analysis of monoclonal antibodies is usually aimed at separating monomers from dimers, trimers and other aggregates, as these can have a negative impact on the effectiveness of biopharmaceuticals. Furthermore, various fragments of mAb's can also be analysed, e.g. Fc or Fab fragments. The analysis of monoclonal antibodies can be carried out using different chromatographic separation techniques.

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Technical Data

SEC is a very suitable and widely used method for the determination of aggregate, monomer and fragment contents of mAb's in analyte solutions. The following manufacturers offer suitable columns for this purpose:

Tosoh Bioscience™

TSKgel G3000SWXL
TSKgel UP-SW3000
TSKgel SuperSW mAb HR
TSKgel SuperSW mAb HTP
TSKgel UltraSW units

Applications:

1. Size Exclusion Chromatography (SEC)

Sepax™ Technologies

SRT-10, SRT-10C
SRT, SRT-C
Zenix, Zenix-C
Unix, Unix-C

Applications:

Shodex™

PROTEIN LW-803

Applications:

Shodex PROTEIN LW-803 Comparison if SEC Separation of mAb Applications

2. Hydrophobic Interaction Chromatography (HIC)

The separation in hydrophilic interaction chromatography is based on the different surface hydrophobicity of various proteins. If this is the case for the respective analytes, HIC can be used to separate different antibodies from each other, to separate antibodies from other proteins and also to separate antibody aggregates from monomers. The separation of antibody fragments is also possible. The following manufacturers offer suitable columns for this purpose:

Tosoh Bioscience™

TSKgel Phenyl-5PW
TSKgel Ether-5PW
TSKgel Butyl-NPR

The hydrophobicity of the phases mentioned decreases in the following order: Phenyl-5PW > Ether-5PW > Butyl-NPR

Applications:

Sepax™ Technologies

Proteomix HIC Ethyl
Proteomix HIC Propyl
Proteomix HIC Butyl
Proteomix HIC Phenyl

Applications:

3. Ion Exchange Chromatography

Ion exchange chromatography can be used to separate different antibodies, fragments, aggregates and other proteins.

Tosoh Bioscience™

TSKgel SP-STAT - A strong cation exchanger based on non-porous polymethacrylate particles
TSKgel CM-STAT - A weak cation exchanger based on non-porous polymethacrylate particles
TSKgel Q-STAT and DNA-STAT - Strong anion exchangers based on non-porous polymethacrylate particles

Applications:

Sepax™ Technologies

Antibodix-NP1.7, -NP3, -NP5 and -NP10 - Weak cation exchanger based on non-porous polystyrene-divinylbenzene particles
Proteomix SCX-NP1.7, -NP3, NP5 and -NP10 - Strong cation exchanger based on non-porous polystyrene-divinylbenzene particles

Applications:

4. Reversed-Phase Chromatography

Different monoclonal antibodies can also be analysed using reverse-earth phase chromatography.

Advanced Materials Technology™

HALO^® 400 Å C4 PROTEIN
HALO^® 1000 Å C4 PROTEIN

Applications:

Tosoh Bioscience™

TSKgel Protein C4-300- A silica-based stationary phase with 300 Å pore size

Applications:

Tosoh TSKgel Protein C4-300 RP Analysis of Antibody Fragments Applications

Sepax™ Technologies

Proteomix RP-300 - Based on polystyrene-divinylbenzene copolymer with 300 Å pore size
Proteomix RP-500 - Based on polystyrene-divinylbenzene copolymer with 500 Å pore size
Proteomix RP-1000 - Based on polystyrene-divinylbenzene copolymer with 1000 Å pore size

Applications:

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