AMT - Advanced Materials Technology / HALO

HALO

HALO^®is a series of (U)HPLC columns based on the core-shell (FusedCore™) technology. The manufacturer of these columns is Advanced Materials Technology™ (AMT), whose founders was outstanding pioneers from the field of high-performance liquid chromatography. AMT is the genuine developer and producer of of packing materials with FusedCore™ technology. The silica-based stationary phases consist of a non-porous core and an porous outer layer, that provides fast separations without excessive backpressure. HALO^® columns are available in different particle sizes: 2 μm (core: 1.2 μm), 2.7 μm (core: 1.7 μm), 3.4 μm (only HALO^® Protein columns, core: 3.0 μm) and 4.6 μm (core: 3.3 μm) and with various modifications that cover a wide selectivity range. HALO^® Bioclass columns have been specially designed for the separation of biomolecules such as proteins, peptides or glycans. HALO^® Enviroclass columns are designed for environmental analysis.

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Halo RP-Amide 90Å 2.7µm 50x10mm CoreShell Column 1 pc

AMT-92810-407

€2,250.00

Product Type: CoreShell Column Bet Surface Area [m2/g]: 150.0 Carbon Load: 8.2 % Delivery Time: approx. 5 days Chemistry: Polar embedded, Alkylamide Column Type: Semi-preparative Column Endcapping: endcapped Manufacturer: Advanced Materials Technology Hardware Type: Ready-to-use Column Matrix: Silica Internal Diameter: 10 mm Length: 50 mm Particle Morphology: spherical, solid core, porous shell pH Minimum: 2.0 pH Maximum: 9.0 Pressure Stability: 600 bar Pore Size: 90 Å Technology: HPLC USP Class: L60

Halo RP-Amide 90Å 2µm 250x3.0mm CoreShell Column 1 pc

AMT-91813-907

€1,090.00

Product Type: CoreShell Column Bet Surface Area [m2/g]: 120.0 Carbon Load: 7.3 % Delivery Time: approx. 5 days Chemistry: Polar embedded, Alkylamide Column Type: Analytical Column Endcapping: endcapped Manufacturer: Advanced Materials Technology Hardware Type: Ready-to-use Column Matrix: Silica Internal Diameter: 3.0 mm Length: 250 mm Particle Morphology: spherical, solid core, porous shell pH Minimum: 2.0 pH Maximum: 9.0 Pressure Stability: 1000 bar Pore Size: 90 Å Technology: HPLC USP Class: L60

Halo RP-Amide 90Å 2µm 250x2.1mm CoreShell Column 1 pc

AMT-91812-907

€1,090.00

Product Type: CoreShell Column Bet Surface Area [m2/g]: 120.0 Carbon Load: 7.3 % Delivery Time: approx. 5 days Chemistry: Polar embedded, Alkylamide Column Type: Analytical Column Endcapping: endcapped Manufacturer: Advanced Materials Technology Hardware Type: Ready-to-use Column Matrix: Silica Internal Diameter: 2.1 mm Length: 250 mm Particle Morphology: spherical, solid core, porous shell pH Minimum: 2.0 pH Maximum: 9.0 Pressure Stability: 1000 bar Pore Size: 90 Å Technology: HPLC USP Class: L60

Halo RP-Amide 90Å 2µm 150x3.0mm CoreShell Column 1 pc

AMT-91813-707

€855.00

Product Type: CoreShell Column Bet Surface Area [m2/g]: 120.0 Carbon Load: 7.3 % Delivery Time: approx. 5 days Chemistry: Polar embedded, Alkylamide Column Type: Analytical Column Endcapping: endcapped Manufacturer: Advanced Materials Technology Hardware Type: Ready-to-use Column Matrix: Silica Internal Diameter: 3.0 mm Length: 150 mm Particle Morphology: spherical, solid core, porous shell pH Minimum: 2.0 pH Maximum: 9.0 Pressure Stability: 1000 bar Pore Size: 90 Å Technology: HPLC USP Class: L60

Halo RP-Amide 90Å 2µm 100x3.0mm CoreShell Column 1 pc

AMT-91813-607

€790.00

Product Type: CoreShell Column Bet Surface Area [m2/g]: 120.0 Carbon Load: 7.3 % Delivery Time: approx. 5 days Chemistry: Polar embedded, Alkylamide Column Type: Analytical Column Endcapping: endcapped Manufacturer: Advanced Materials Technology Hardware Type: Ready-to-use Column Matrix: Silica Internal Diameter: 3.0 mm Length: 100 mm Particle Morphology: spherical, solid core, porous shell pH Minimum: 2.0 pH Maximum: 9.0 Pressure Stability: 1000 bar Pore Size: 90 Å Technology: HPLC USP Class: L60

Halo RP-Amide 90Å 2µm 75x3.0mm CoreShell Column 1 pc

AMT-91813-507

€770.00

Product Type: CoreShell Column Bet Surface Area [m2/g]: 120.0 Carbon Load: 7.3 % Delivery Time: approx. 5 days Chemistry: Polar embedded, Alkylamide Column Type: Analytical Column Endcapping: endcapped Manufacturer: Advanced Materials Technology Hardware Type: Ready-to-use Column Matrix: Silica Internal Diameter: 3.0 mm Length: 75 mm Particle Morphology: spherical, solid core, porous shell pH Minimum: 2.0 pH Maximum: 9.0 Pressure Stability: 1000 bar Pore Size: 90 Å Technology: HPLC USP Class: L60

Halo RP-Amide 90Å 2µm 50x3.0mm CoreShell Column 1 pc

AMT-91813-407

€755.00

Product Type: CoreShell Column Bet Surface Area [m2/g]: 120.0 Carbon Load: 7.3 % Delivery Time: approx. 5 days Chemistry: Polar embedded, Alkylamide Column Type: Analytical Column Endcapping: endcapped Manufacturer: Advanced Materials Technology Hardware Type: Ready-to-use Column Matrix: Silica Internal Diameter: 3.0 mm Length: 50 mm Particle Morphology: spherical, solid core, porous shell pH Minimum: 2.0 pH Maximum: 9.0 Pressure Stability: 1000 bar Pore Size: 90 Å Technology: HPLC USP Class: L60

Halo RP-Amide 90Å 2µm 30x3.0mm CoreShell Column 1 pc

AMT-91813-307

€755.00

Product Type: CoreShell Column Bet Surface Area [m2/g]: 120.0 Carbon Load: 7.3 % Delivery Time: approx. 5 days Chemistry: Polar embedded, Alkylamide Column Type: Analytical Column Endcapping: endcapped Manufacturer: Advanced Materials Technology Hardware Type: Ready-to-use Column Matrix: Silica Internal Diameter: 3.0 mm Length: 30 mm Particle Morphology: spherical, solid core, porous shell pH Minimum: 2.0 pH Maximum: 9.0 Pressure Stability: 1000 bar Pore Size: 90 Å Technology: HPLC USP Class: L60

Halo RP-Amide 90Å 2µm 20x3.0mm CoreShell Column 1 pc

AMT-91813-207

€755.00

Product Type: CoreShell Column Bet Surface Area [m2/g]: 120.0 Carbon Load: 7.3 % Delivery Time: approx. 5 days Chemistry: Polar embedded, Alkylamide Column Type: Analytical Column Endcapping: endcapped Manufacturer: Advanced Materials Technology Hardware Type: Ready-to-use Column Matrix: Silica Internal Diameter: 3.0 mm Length: 20 mm Particle Morphology: spherical, solid core, porous shell pH Minimum: 2.0 pH Maximum: 9.0 Pressure Stability: 1000 bar Pore Size: 90 Å Technology: HPLC USP Class: L60

Halo RP-Amide 90Å 2µm 5x3.0mm HPLC Guard Cartridges 3 pcs

AMT-91813-107

€565.00

Product Type: HPLC Guard Cartridges Bet Surface Area [m2/g]: 120.0 Carbon Load: 7.3 % Delivery Time: approx. 5 days Chemistry: Polar embedded, Alkylamide Endcapping: endcapped Manufacturer: Advanced Materials Technology Hardware Type: Cartridges Matrix: Silica Internal Diameter: 3.0 mm Length: 5 mm Particle Morphology: spherical, solid core, porous shell pH Minimum: 2.0 pH Maximum: 9.0 Pressure Stability: 1000 bar Pore Size: 90 Å Technology: HPLC USP Class: L60

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Technical Data

HALO® 2 HALO® 2.7 and HALO® 5 (U)HPLC columns:

Phase name	Modification	Particle size	Pore size	End-capping	C-content	USP	Surface area	pH range	Tmax
HALO^® C30	C₃₀	2.7 µm	160 Å	+	4.5%	L62	90m²/g	2.0-9.0	60°C^a 40°C^b
HALO^® C18	C₁₈	2 µm 2.7 µm 5 µm	90 Å	+	7.5% 7.7% 5.4%	L1	120m²/g 135m²/g 90m²/g	2.0-9.0	60°C^a 40°C^b
HALO LPH-C18	C₁₈	2 µm 2.7 µm	90 Å	-	6.5%	L1	120m²/g 135m²/g	1.0-8.0	90°C^a 40°C^b
HALO^® AQ-C18	C₁₈	2 µm 2.7 µm 5 µm	90 Å	+	6.5% 6.7% 5.6%	L1	120m²/g 135m²/g 90m²/g	2.0-9.0	60°C^a 40°C^b
HALO^® PCS-C18	positively charged surface C₁₈	2.7 µm	90 Å	+	7.5%	L1	125 m²/g	2.0-7.0	60°C^a 40°C^b
HALO^® C8	C₈	2 µm 2.7 µm 5 µm	90 Å	+	4.8% 5.4% 3.7%	L7	120m²/g 135m²/g 90m²/g	2.0-9.0	60°C^a 40°C^b
HALO^® Phenyl-Hexyl	Phenylhexyl	2 µm 2.7 µm 5 µm	90 Å	+	6.3% 7.1% 5.2%	L11	120m²/g 135m²/g 90m²/g	2.0-9.0	60°C^a 40°C^b
HALO^® Biphenyl	Biphenyl	2.7 µm	90 Å	+	7.0 %	L11	135m²/g	2.0-9.0	60°C^a 40°C^b
HALO^® PCS Phenyl-Hexyl	positively charged surface Phenyl-Hexyl	2.7 µm	90 Å	+	6.1%	L11	125 m²/g	2.0-7.0	60°C^a 40°C^b
HALO^® ES-CN	Diisopropylcyanopropyl	2 µm 2.7 µm 5 µm	90 Å	+	3.5% 3.5% 2.5%	L10	120m²/g 135m²/g 90m²/g	1.0-8.0	80°C^a 40°C^b
HALO^® PFP	Pentafluorophenylpropyl	2 µm 2.7 µm 5 µm	90 Å	+	5.3% 5.5% 3.9%	L43	120m²/g 135m²/g 90m²/g	2.0-9.0	60°C^a 40°C^b
HALO^® RP-Amide	C₁₆ Amide	2 µm 2.7 µm 5 µm	90 Å	+	7.3% 8.2% 5.5%	L60	120m²/g 135m²/g 90m²/g	2.0-9.0	60°C^a 40°C^b
HALO^® HILIC	-	2 µm 2.7 µm 5 µm	90 Å	N/A	-	L3	120m²/g 135m²/g 90m²/g	1.0-8.0	60°C^a 40°C^b
HALO^® Penta-HILIC	Penta-hydroxy ligand	2 µm 2.7 µm 5 µm	90 Å	-	2.8% 3.2% 2.1%	L95	120m²/g 135m²/g 90m²/g	2.0-9.0	60°C^a 40°C^b

a low pH; b high pH

HALO® Bioclass Columns

Phase name	Modification	Particle size	Pore size	End-capping	C-content	USP	Surface area	pH range	Tmax
HALO^® Protein ES-C18	C₁₈	2.7 µm 3.4 µm	1000 Å 400 Å	+	1.4% 1.0%	L1	15 m²/g	1.0-8.0	90°C^a 40°C^b
HALO^® Protein C4	C₄	2.7 µm 3.4 µm	1000 Å 400 Å	+	0.6% 0.4%	L26	15 m²/g	2.0-9.0	90°C^a 40°C^b
HALO^® Protein Diphenyl	Diphenylmethyl	2.7 µm 3.4 µm	1000 Å 400 Å	+	N/A 1.0%	L11	15 m²/g	2.0-9.0	90°C^a 40°C^b
HALO^® Peptide ES-C18	C₁₈	2 µm 2.7 µm 5 µm	160 Å	-	4.0% 4.6% 4.0%	L1	65 m²/g 90 m²/g 60 m²/g	1.0-8.0	90°C^a 40°C^b
HALO^® PCS-C18	positively charged surface C₁₈	2.7 µm	160 Å	+	5.1%	L1	125 m²/g	2.0-7.0	60°C^a 40°C^b
HALO^® Peptide ES-CN	Diisopropylcyanopropyl	2.7 µm 5 µm	160 Å	+	2.2% 1.5%	L10	90 m²/g 60 m²/g	1.0-8.0	90°C^a 40°^Cb
HALO^® Peptides Phenyl-hexyl	Phenylhexyl	2.7 µm	160 Å	+	4.7%	L11	90 m²/g	2.0-9.0	90°C^a 40°C^b
HALO^® Glycan	Penta-hydroxy ligand	2.7 µm	160 Å	-	3.2%	N/A	135 m²/g	2.0-9.0	65°C^a 40°C^b

a low pH; b high pH

HALO® Enviroclass columns:

Phase name	Modification	Particle size	Pore size	End-capping	C-content	USP	Surface area	pH range	Tmax
HALO^® PAH	C₁₈	2.7 µm	90 Å	-	9.9%	N/A	135m²/g	2.0-9.0	60°C^a 40°C^b
HALO^® PFAS	C₄	2.7 µm	90 Å	+	N/A	N/A	135m²/g	2.0-9.0	60°C^a 40°C^b
HALO^® PFAS Delay	Proprietary	2.7 µm	90 Å	+	N/A	N/A	90m²/g	2.0-9.0	60°C^a 40°C^b

a low pH; b high pH

Applications

HALO® Biphenyl

The HALO® Biphenyl is ready for the challenge of complex samples. Both polar and non-polar pesticides are well resolved with this commonly used test for cannabis.

ADVANTAGES OF THE NEW HALO® BIPHENYL

Improved retention, high sensitivity and higher efficiency without sacrificing robustness or reproducibility
Increased selectivity for challenging polar compounds
High efficiency leading to sharply resolved peaks with excellent peak shapes

Peak Identities

1 Daminozide 2 Flonicamid 3 Thiamethoxam 4 Imidacloprid 5 Paclobutrazol 6 Fenhexamid
7 Myclobutanil 8 Bifenazate 9 Dimethomorph Isomer 1 10 Pirotetramat 11 Dimethomorph Isomer 2
12 Spinosad A 13 Spinosad D 14 Trifloxystrobin 15 Spinetoram 16 Pyrethrin II 17 Piperonyl butoxide
18 Pyrethrin I 19 Etoxazole 20 Abamectin A 21 Cypermethrin 22 Bifenthrin 23 Acequinocyl

Isomer match

Tocopherols, a form of vitamin E known for its antioxidant properties, contain many isomers that can be easily separated in the baseline on the HALO® C30 compared to a C18 phase due to the phase's shape selectivity.

PEAKIDENTITIES

1. δ-tocopherol 2. γ- tocopherol 3. β- tocopherol 4. α- tocophero

TEST CONDITIONS

Column: HALO 160 Å C30 and 90 Å C18 , 2.7 µm, 4.6 x 150mm
Mobile Phase A: Water
Mobile Phase B: Methanol
Isocratic: 95% B
Flow rate: 1.5 mL/min
Temperature: 10°C Injection
Injection volume: 1.5 μL
Instrument: Agilent 1200 SL
Detection: UV 290 nm, PDA

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HALO

Product Line

Products

Technical Data

HALO® 2 HALO® 2.7 and HALO® 5 (U)HPLC columns:

HALO® Bioclass Columns

HALO® Enviroclass columns:

Applications

HALO® Biphenyl

Isomer match

Downloads

Brochures

Contact Us

The right column for you - we will be happy to support you individually

You will find:

Write us a message and we will get back to you as soon as possible.

Gradient:	Time [min]	B [%]
	0.00	0
	1.01	15
	4.00	35
	5.00	62
	30.00	100
	34.00	100