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HALO INERT is a new inert hardware for HPLC. A metal-free barrier between the sample and the hardware minimises unwanted interactions between the analytes and the stainless steel surface. This hardware is ideal for phosphorylated samples and analytes that can adsorb to stainless steel. These include many biomolecules such as oligonucleotides, nucleotides and some proteins and peptides. This new hardware improves the peak shape and recovery in the samples for such analytes compared to a standard stainless steel column.