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HALO Elevate C18
HALO Elevate C18 is an HPLC column from Advanced Materials Technology (AMT) with an organo-silane modified surface. This hybrid material is therefore stable at a pH value between 2 and 12. Due to this high pH stability, bases can be analysed uncharged, resulting in good peak symmetries. The modified surface is based on AMT's well-known fused-core technology, so that high speed is achieved in combination with high pH stability.
From now until 20.12.24 you will receive a -40% introductory discount on the new HALO Elevate C18 columns! Please note that guard columns are excluded from this promotion.
Technical Data
| Phase | Modification | Particle size | Pore size | Surface area | C-content | Endcapped | USP | pH range | Tmax |
| HALO Elevate C18 | Diisobutyloctadecylsilanes | 2.7 µm | 120 Å | 75 m²/g | 5.6 %C | + | L1 | 2.0-12.0 | 60°Ca |
a: for low and high pH range
Applications
What are the advantages of the HALO Elevate C18?
Due to the organo-silane modified surface, these columns are very stable at high pH values. Amitriptyline was analysed at a pH value of 10. It was found that the retention only shifted by 1% after 500 injections (or 20,000 column volumes).
Test Conditions
Column: HALO 120Å Elevate C18, 2.7µm, 50x2.1mm (92272-402)
Analytes: 1. Uracil 2. Acenaphthen 3. Amitriptylin
Mobile Phase A: 95/5 10mM Ammonium bicarbonate pH 10 / Acetonitrile
Mobile Phase B: Acetonitrile
Gradient:
Time / min | %B |
0.0 | 0 |
2.5 | 95 |
3.0 | 95 |
3.1 | 0 |
5.0 | 0 |
Flow rate: 0.8mL/min
Back pressure: 220bar
Temperature: 60°C
Detection: UV/PDA, 260nm
Injection volume: 1.0µL
LC System: Shimadzu Nexera X2
When will you benefit from the HALO Elevate C18?
- Whenever a high pH value is advantageous (alkaline analytes)
Analysis of Omeprazole
When analysing omeprazole (pka 9.3), related compounds and impurities, the run time can be reduced by more than 84% compared to the original USP method. All 9 component are baseline separated.
Peak Identities
1. Related Compound F & G 2. Related Compound B 3. Related Compound E 4. Related Compound A 5. Impurity B 6. Omeprazole 7. Impurity H 8. N'-Methyl Omeprazole 9. Impurity C
Analysis of Tricyclic Antidepressants
Tricyclic antidepressants (TCAs) are basic drugs that can be separated with the HALO Elevate C18 in less than 3 min with excellent peak symmetries.
Peak Identities
1. Nortriptyline 2. Doxepin 3. Imipramine 4. Amitriptyline 5. Trimipramine
Conclusion: With the hybrid-based HALO Elevate C18, basic analytes can be analysed in the uncharged state. This avoids secondary interactions, resulting in high peak symmetries. This column is ideal for laboratories with many basic applications.
Downloads
Brochures
AMT HALO Elevate C18 Brochure
Applications
AMT HALO Elevate C18 Amitriptyline Stability Test Applications
AMT HALO Elevate C18 Nicotine Analysis Application
AMT HALO Elevate C18 Omeprazole Analysis Application
AMT HALO Elevate C18 Separation of Antidepressants Applications
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