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HALO
HALO® is a series of (U)HPLC columns based on the core-shell (FusedCore™) technology. The manufacturer of these columns is Advanced Materials Technology™ (AMT), whose founders was outstanding pioneers from the field of high-performance liquid chromatography. AMT is the genuine developer and producer of of packing materials with FusedCore™ technology. The silica-based stationary phases consist of a non-porous core and an porous outer layer, that provides fast separations without excessive backpressure. HALO® columns are available in different particle sizes: 2 μm (core: 1.2 μm), 2.7 μm (core: 1.7 μm), 3.4 μm (only HALO® Protein columns, core: 3.0 μm) and 4.6 μm (core: 3.3 μm) and with various modifications that cover a wide selectivity range. HALO® Bioclass columns have been specially designed for the separation of biomolecules such as proteins, peptides or glycans. HALO® Enviroclass columns are designed for environmental analysis.
Technical Data
HALO® 2 HALO® 2.7 and HALO® 5 (U)HPLC columns:
| Phase name | Modification | Particle size | Pore size | End-capping | C-content | USP | Surface area | pH range | Tmax |
| HALO® C30 | C30 | 2.7 µm | 160 Å | + | 4.5% | L62 | 90m2/g | 2.0-9.0 | 60°Ca |
| HALO® C18 | C18 | 2 µm | 90 Å | + | 7.5% | L1 | 120m2/g | 2.0-9.0 | 60°Ca |
| HALO LPH-C18 | C18 | 2 µm | 90 Å | - | 6.5% | L1 | 120m2/g | 1.0-8.0 | 90°Ca |
| HALO® AQ-C18 | C18 | 2 µm | 90 Å | + | 6.5% | L1 | 120m2/g | 2.0-9.0 | 60°Ca |
| HALO® PCS-C18 | positively charged surface C18 | 2.7 µm | 90 Å | + | 7.5% | L1 | 125 m²/g | 2.0-7.0 | 60°Ca |
| HALO® C8 | C8 | 2 µm | 90 Å | + | 4.8% | L7 | 120m2/g | 2.0-9.0 | 60°Ca |
| HALO® Phenyl-Hexyl | Phenylhexyl | 2 µm | 90 Å | + | 6.3% | L11 | 120m2/g | 2.0-9.0 | 60°Ca |
| HALO® Biphenyl | Biphenyl | 2.7 µm | 90 Å | + | 7.0 % | L11 | 135m2/g | 2.0-9.0 | 60°Ca |
| HALO® PCS Phenyl-Hexyl | positively charged surface Phenyl-Hexyl | 2.7 µm | 90 Å | + | 6.1% | L11 | 125 m²/g | 2.0-7.0 | 60°Ca |
| HALO® ES-CN | Diisopropylcyanopropyl | 2 µm | 90 Å | + | 3.5% | L10 | 120m2/g | 1.0-8.0 | 80°Ca |
| HALO® PFP | Pentafluorophenylpropyl | 2 µm | 90 Å | + | 5.3% | L43 | 120m2/g | 2.0-9.0 | 60°Ca |
| HALO® RP-Amide | C16 Amide | 2 µm | 90 Å | + | 7.3% | L60 | 120m2/g | 2.0-9.0 | 60°Ca |
| HALO® HILIC | - | 2 µm | 90 Å | N/A | - | L3 | 120m2/g | 1.0-8.0 | 60°Ca |
| HALO® Penta-HILIC | Penta-hydroxy ligand | 2 µm | 90 Å | - | 2.8% | L95 | 120m2/g | 2.0-9.0 | 60°Ca |
a low pH; b high pH
HALO® Bioclass Columns
| Phase name | Modification | Particle size | Pore size | End-capping | C-content | USP | Surface area | pH range | Tmax |
| HALO® Protein ES-C18 | C18 | 2.7 µm | 1000 Å | + | 1.4% | L1 | 15 m2/g | 1.0-8.0 | 90°Ca |
| HALO® Protein C4 | C4 | 2.7 µm | 1000 Å | + | 0.6% | L26 | 15 m2/g | 2.0-9.0 | 90°Ca |
| HALO® Protein Diphenyl | Diphenylmethyl | 2.7 µm | 1000 Å | + | N/A | L11 | 15 m2/g | 2.0-9.0 | 90°Ca |
| HALO® Peptide ES-C18 | C18 | 2 µm | 160 Å | - | 4.0% | L1 | 65 m2/g | 1.0-8.0 | 90°Ca |
| HALO® PCS-C18 | positively charged surface C18 | 2.7 µm | 160 Å | + | 5.1% | L1 | 125 m²/g | 2.0-7.0 | 60°Ca 40°Cb |
| HALO® Peptide ES-CN | Diisopropylcyanopropyl | 2.7 µm | 160 Å | + | 2.2% | L10 | 90 m2/g | 1.0-8.0 | 90°Ca |
| HALO® Peptides Phenyl-hexyl | Phenylhexyl | 2.7 µm | 160 Å | + | 4.7% | L11 | 90 m2/g | 2.0-9.0 | 90°Ca |
| HALO® Glycan | Penta-hydroxy ligand | 2.7 µm | 160 Å | - | 3.2% | N/A | 135 m2/g | 2.0-9.0 | 65°Ca |
a low pH; b high pH
HALO® Enviroclass columns:
| Phase name | Modification | Particle size | Pore size | End-capping | C-content | USP | Surface area | pH range | Tmax |
| HALO® PAH | C18 | 2.7 µm | 90 Å | - | 9.9% | N/A | 135m2/g | 2.0-9.0 | 60°Ca |
| HALO® PFAS | C4 | 2.7 µm | 90 Å | + | N/A | N/A | 135m2/g | 2.0-9.0 | 60°Ca |
| HALO® PFAS Delay | Proprietary | 2.7 µm | 90 Å | + | N/A | N/A | 90m2/g | 2.0-9.0 | 60°Ca |
a low pH; b high pH
Applications
HALO® Biphenyl
The HALO® Biphenyl is ready for the challenge of complex samples. Both polar and non-polar pesticides are well resolved with this commonly used test for cannabis.
ADVANTAGES OF THE NEW HALO® BIPHENYL
- Improved retention, high sensitivity and higher efficiency without sacrificing robustness or reproducibility
- Increased selectivity for challenging polar compounds
- High efficiency leading to sharply resolved peaks with excellent peak shapes
Peak Identities
1 Daminozide 2 Flonicamid 3 Thiamethoxam 4 Imidacloprid 5 Paclobutrazol 6 Fenhexamid
7 Myclobutanil 8 Bifenazate 9 Dimethomorph Isomer 1 10 Pirotetramat 11 Dimethomorph Isomer 2
12 Spinosad A 13 Spinosad D 14 Trifloxystrobin 15 Spinetoram 16 Pyrethrin II 17 Piperonyl butoxide
18 Pyrethrin I 19 Etoxazole 20 Abamectin A 21 Cypermethrin 22 Bifenthrin 23 Acequinocyl
Isomer match
Tocopherols, a form of vitamin E known for its antioxidant properties, contain many isomers that can be easily separated in the baseline on the HALO® C30 compared to a C18 phase due to the phase's shape selectivity.
PEAKIDENTITIES
- 1. δ-tocopherol 2. γ- tocopherol 3. β- tocopherol 4. α- tocophero
TEST CONDITIONS
- Column: HALO 160 Å C30 and 90 Å C18 , 2.7 µm, 4.6 x 150mm
- Mobile Phase A: Water
- Mobile Phase B: Methanol
- Isocratic: 95% B
- Flow rate: 1.5 mL/min
- Temperature: 10°C Injection
- Injection volume: 1.5 μL
- Instrument: Agilent 1200 SL
- Detection: UV 290 nm, PDA
Downloads
Brochures
AMT HALO Enviroclass - HALO-PFAS Environmental Analysis
AMT HALO MZ-Apps Rp HALO 1000Å Brochure
AMT HALO AQ-C18 Columns Brochure
AMT Enhanced Selectivity With the HALO 160Å Phenyl-Hexyl Column Brochure
AMT HALO Bioclass Brochure
AMT HALO C30 Columns Brochure
AMT HALO® Catalog 2023
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