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HALO LPH-C18
The new HALO® LPH-C18 column from Advanced Material Technology (AMT) is a sterically protected C18 phase based on a core-shell material for low pH values. This steric protection reduces the acidic hydrolysis, making the columns suitable for pH up to 1. The particles are available in 2 and 2.7 μm and in lengths up to 150 mm. Moreover, the 2.7 μm particles are also available in the new dimension of 1.5 mm ID.
Technical Data
| Phase | Modification | Particle size / μm | Pore size / Å | Carbon content / %C | Surface area / m²/g | Endcapped | pH | USP Code |
| HALO-LPH C18 | Diisobutyloctadecylsilane | 2 | 90 | 6.5 | 120 | - | 1a-8b | L1 |
(a) Tmax: 90 °C; (b) Tmax: 40 °C
Applications
What distinguishes the HALO® LPH-C18?
- highly stable at low pH values at high temperatures
- high lot-to-lot reproducibility
HALO 90 Å LPH-C18, 2.7 µm 2.1x50 mm
A separation of parabens is performed on a HALO 90 Ã… LPH-C18 column under low pH (pH 1) and high temperature conditions compared to a standard C18 SPP column. Due to the sterically protected ligand, the LPH-C18 column can withstand these harsh conditions over a 55 hour test.
TEST CONDITIONS:
Column: HALO 90 Å LPH-C18, 2.7 µm 2.1x50 mm
Part Number: 92822-416
Mobile Phase A: Water, 1% TFA (pH: 1)
Mobile Phase B: Acetonitrile
| Gradient: | Time | %B |
| 0.0 | 20 | |
| 7.50 | 20 | |
| 7.51 | 5 | |
| 45.00 | 5 | |
| 47.00 | 100 | |
| 51.00 | 100 | |
| 51.01 | 20 | |
| 60.00 | 20 |
HALO 90 Å LPH-C18 2.7µm 4.6 x 50 mm
Excellent lot-to-lot reproducibility is observed with a mixture of neutral compounds.
TEST CONDITIONS:
Column: HALO 90 Å LPH-C18 2.7µm 4.6 x 50mm
Mobile Phase A: Water
Mobile Phase B: Acetonitrile
Isocratic: 60/40 Acetonitrile/Water
Wavelength: 254 nm
Injection: 2.0 µL (uracil, phenol, 1-Cl-4-nitrobenzene, naphthalene)
Temperature: 30 °C
Flow Cell: 1.8 mL/min.
When do you benefit from the HALO® LPH-C18?
You can benefit from the HALO® LPH-C18 in applications which require a low pH value. Especially in applications containing polyphenols, cannabinoids and pesticides.
HALO 90 Å LPH-C18, 2.7 µm 2.1 x 100 mm
Common polyphenols found in wine are separated using a HALO 90 Ã… LPH-C18 column using analytical standards. This stationary phase contains a sterically protected ligand which is ideal for high stability under low pH conditions.
PEAK IDENTITIES
1. Gallic Acid 2. Epigallocatechin 3. Chlorogenic Acid 4. Catechin 5. Caffeic Acid 6. Epicatechin 7. Epigallocatechin Gallate 8. p-Coumaric Acid 9. Ferulic Acid 10. o-Coumaric Acid 11. Quercitrin 12. Myricetin 13. Resveratrol 14. Morin 15. Quercetin 16. Naringenin 17. Apigenin 18. Kaempfero
HALO 90 Å LPH-C18, 2.7 µm, 4.6 x 150 mm
Common polyphenols found in wine are separated using a HALO 90 Ã… LPH-C18 column using analytical standards. This stationary phase contains a sterically protected ligand which is ideal for high stability under low pH conditions.
PEAK IDENTITIES
1. Cannabidivarinic acid (CBDVA) 2. Cannabidivarin (CBDV) 3. Cannabidiolic acid (CBDA) 4. Cannabigerolic acid (CBGA) 5. Cannabigerol (CBG) 6. Cannabidiol (CBD) 7. Tetrahydrocannabivarin (THCV) 8. Tetrahydrocannabivarinic acid (THCVA) 9. Cannabinol (CBN) 10. Cannabinolic acid (CBNA) 11. Exo-tetrahydrocannabinol (EXO-THC) 12. delta 9- Tetrahydrocannabinol (D9-THC) 13. delta 8- Tetrahydrocannabinol (D8-THC) 14. Cannabicycol (CBL) 15. Cannabichromene (CBC) 16. Tetrahydrocannabinolic acid A (THCA-A) 17. Cannabichromenic acid (CBCA) 18. Cannabicyclolic acid (CBLA)
HALO 90 Å LPH-C18 2 µm, 2.1 x 100 mm
Pesticides screening of crops, in this example barley being the crop of concern, is performed using LCMS with the 2 µm HALO 90 Å LPH-C18 where both speed and resolution are demonstrated.
PEAK IDENTITIES
1. Carbendazim 2. Dicrotophos 3. Azamethiphos 4. Pyrimethani 5. Carbofuran 6. Dodemorph 7. Atrazine 8. Diuron 9. Iprovalicarb 10. Azoxystrobin 11. Fluopram 12. Methoxyfenozide 13. Flutolanil 14. Picoxystrobin
Conclusion: The HALO® LPH-C18 is suitable for applications at low pH values and also offers fast, efficient and rugged separations based on the core-shell technology.
As AMT's exclusive distributor in Germany, you can get the new HALO® LPH-C18 directly from us. We would be pleased to advise and support you in the selection of the suitable column. Contact our support team for a consultation and an individual quote. We are looking forward to your contact!
Downloads
Brochures
AMT, HALO, LPH - C18 Columns
Applications
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AMT HALO Pharmaceuticals Low pH Stability with HALO LPH-C18 Columns
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AMT HALO Separation of Anthocyanins in Blueberries Application
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AMT HALO Separation of Polyphenols in Wine Application
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AMT HALO 90Ã… LPH C18 Pesticide Screening of Barley Application
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AMT HALO Food & Beverage LC-MS Separation of Common Catechins Found in Tea
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AMT HALO Separation of Common Catechins & Caffeine Found in Tea via LC-UV Application
Technical support
AMT HALO Cannabinoid Separations and the Impact of LC Column Selectivity
The right column for you - we will be happy to support you individually
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