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Acrylamide

Acrylamide is formed when food is heated to high temperatures, e.g. when frying or deep-frying. The amino acid asparagine reacts with reducing sugars such as glucose or fructose (Figure 1). Cereal products (cereals, baked goods, etc.), potato products (chips, crisps) and coffee are particularly affected.

 

Animal experiments have shown that acrylamide is carcinogenic and damages the genome. It has therefore been categorised as "probably carcinogenic to humans". However, there are no binding limits for acrylamide in food (in the EU). Only guide values and minimisation measures were defined in 2013 and set out in a regulation at the end of 2017. The guideline values were lowered again. This guideline value is determined by the acrylamide value, which 90% of the samples fell below. There may therefore be further lower guideline values in the future. As a result, analysing acrylamide in food has also become essential.

Figure 1: Formation of acrylamide.

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Oasis HLB 6 cc Vac Cartridge, 200 mg Sorbent per Cartridge, 30 µm Particle Size, 30/pk
WAT106202
€191.00
Acrodisc, Syringe Filter, PVDF, 13 mm, 0.45 µm, Protein, 100/pkg
WAT200512
€395.00
LCMS Certified Clear Glass 12 x 32mm Screw Neck Vial, with Cap and PTFE/silicone Septum , 2 mL Volume, 100/pkg
600000751CV
€77.00
TruView LCMS Certified Clear Glass 12 x 32mm Screw Neck Vial, with Cap and Preslit PTFE/Silicone Septa, 2 mL Volume, 100/pkg
186005666CV
€101.00
XBridge BEH Amide Column, 130Å, 3.5 µm, 4.6 mm X 250 mm, 1/pkg
186004870
€1,050.00
Acquity® UPLC BEH Amide Column, 130Å, 1.7 µm, 2.1 mm X 150 mm, 1/pkg
186004802
€1,090.00
Acquity® UPLC HSS C18 SB Column, 100Å, 1.8 µm, 2.1 mm X 100 mm, 1/pkg
186004119
€1,020.00
Atlantis dc18 Column, 100Å, 3 µm, 2.1 mm X 150 mm, 1/pkg
186001299
€930.00
Atlantis dc18 Column, 100Å, 5 µm, 2.1 mm X 100 mm, 1/pkg
186001297
€830.00
Oasis MCX 3 cc Vac Cartridge, 60 mg Sorbent per Cartridge, 30 µm Particle Size, 100/pk
186000254
€415.00
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Applications

Reversed-phase chromatography

Analysis of acrylamide, acrylonitrile and organic acids with GL Sciences Inertsil ODS-4

Peak identities

1. acrylamides 2. maleic acid 3. acrylic acid 4. acrylonitriles 5. itaconic acid 6. methacrylic acid

Test conditions

Column: Inertsil ODS-4 150x4.6mm, 3µm (5020-04045)

Mobile phase A: Acetonitrile

Mobile phase B: 0.1% phosphoric acid in water

Isocratic: A/B = 3/97, v/v

Flow rate: 1.0 mL/min

Column temperature: 40 °C

Detection: UV 210 nm

Injection volume: 10 µL

HILIC

Analysis of acrylamide, methacrylamide and methyl acrylic acid with Macherey-Nagel Nucleodur HILIC

Peak identities

1. methyl acrylamides 2. acrylamides 3. methylarylic acid

Test conditions

Column: Nucleodur HILIC 125x4.0mm, 5 µm (760551.40)

Mobile phase: Acetonitrile / 0.1% formic acid (98:2, v/v)

Flow rate: 0.6 mL/min

Temperature: 22 °C

Injection volume: 0.5 µL

Detection: UV, 210 nm

Gas chromatography

Analysis of acrylonitrile, acrylamide and propionamide with GL Sciences InertCap Pure-WAX

Peak identities

1. acrylonitriles 2. propionamides 3. acrylamides

Test conditions

Column: InertCap Pure-WAX 30m x 0.25mm, 0.25 µm (1010-68142)

Column temperature: 40 °C (2min hold) - 20 °C/min - 150 °C (9.5min hold)

Carrier gas: Helium 100 kPa

Injection: Split flow 50 mL/min, 200 °C

Detection: FID range 10^0, 250 °C

Sample size: 100 mg/L in methanol, 1 µL

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Based on these developments, many manufacturers have published applications for the determination of acrylamide. These range from reversed-phase chromatography, normal-phase chromatography and HILIC to gas chromatography.

Manufacturer overview

GL Sciences

HPLC - Reversed Phase:

  • Inertsil ODS-4 3µm

 

GC:

  • InertCap Pure-WAX

 

Imtakt

HPLC - Reversed Phase:

  • Unison UK-C18
  • Unison UK-C8
  • Unison UK-Phenyl
  • Cadenza CL-C18

 

HPLC - Normal Phase:

  • Unison UK-Amino
  • Unison UK-Silica

Macherey nail

HPLC - Reversed Phase:

  • Nucleodur C18 Gravity 3µm
  • Nucleodur C18 HTec 5µm
  • Nucleoshell Bluebird RP 18 2.7µm

 

HPLC - HILIC:

  • Nucleodur HILIC 5µm
  • Nucleoshell HILIC 2.7µm

Osaka soda

HPLC - Reversed Phase:

  • Capcell PAK C18 AQ 3µm

 

HPLC - HILIC:

  • Capcell Cpre PC 2.7µm

Perkin-Elmer

UHPLC - Reversed Phase:

  • Altus UPLC BEH C18 1.7µm

Restek

HPLC - Reversed Phase:

  • Allure Acrylamide 5µm

 

GC:

  • Stabilwax

Shimadzu

GC:

  • CP-Sil 24 CB

Shodex

HPLC - Reversed Phase:

  • RSpak DE-413 (polymer column)

Sielc

HPLC - Mixed Mode:

  • Primesep 200 3µm

Merck Supelco

HPLC - Reversed Phase:

  • Discovery HS F5 5µm

 

Sample preparation:

  • Discovery DSC-MCAX
  • Discovery DSC-C18

Thermo Fisher

HPLC - Reversed Phase:

  • Hypercarb 5µm

 

HPLC - Ion chromatography:

  • Dionex IonPac ICE-AS1 7.5µm

 

Gas chromatography:

  • TarceGOLD TG-WaxMS

 

Sample preparation:

  • HyperSep SLE96-well plate
  • HyperSep Hypercarb SPE

Tosoh

HPLC _ Reversed Phase:

  • TSkgel ODS-100V 5µm

 

HPLC - HILIC:

  • TSKgel Amide-80 5µm

Waters

HPLC - Reversed Phase:

  • Atlantis dC18 5µm
  • Atlantis dC18 3µm

 

UHPLC - Reversed Phase:

  • ACQUITY HSS C18 SB 1.8µm

 

HPLC - HILIC:

  • XBridge Amide 3.5µm

 

UHPLC - HILIC:

  • ACQUITY BEH Amide 1.7µm

 

Sample preparation

  • Oasis HLB SPE
  • Oasis MCX SPE

Which

HPLC - Reversed Phase:

  • Ultisil XB-C18 3µm

 

Sample preparation:

  • Welchrom C18E

ZirChrome

HPLC - Reversed Phase:

  • ZirChrom-CARB

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