AMT - Advanced Materials Technology / HALO

HALO

HALO® is a series of (U)HPLC columns based on the core-shell (FusedCore™) technology. The manufacturer of these columns is Advanced Materials Technology™ (AMT), whose founders was outstanding pioneers from the field of high-performance liquid chromatography. AMT is the genuine developer and producer of of packing materials with FusedCore™ technology. The silica-based stationary phases consist of a non-porous core and an porous outer layer, that provides fast separations without excessive backpressure. HALO® columns are available in different particle sizes: 2 μm (core: 1.2 μm), 2.7 μm (core: 1.7 μm), 3.4 μm (only HALO® Protein columns, core: 3.0 μm) and 4.6 μm (core: 3.3 μm) and with  various modifications that cover a wide selectivity range. HALO® Bioclass columns have been specially designed for the separation of biomolecules such as proteins, peptides or glycans. HALO® Enviroclass columns are designed for environmental analysis.

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AMT-9281X-418
€760.00
AMT-92814-117
€565.00
AMT-92813-117
€565.00
AMT-92812-117
€565.00
AMT-92814-917
€1,115.00
AMT-92814-717
€850.00
AMT-92814-617
€760.00
AMT-92814-417
€680.00
AMT-92813-717
€850.00
AMT-92813-617
€760.00
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Technical Data

HALO® 2 HALO® 2.7 and HALO® 5 (U)HPLC columns:

Phase name

Modification

Particle size

Pore size

End-capping

C-content

USP

Surface area

pH range

Tmax

HALO® C30

C30

2.7 µm

160 Å

+

4.5%

L62

90m2/g

2.0-9.0

60°Ca
40°Cb

HALO® C18

C18

2 µm
2.7 µm
5 µm

90 Å

+

7.5%
7.7%
5.4%

L1

120m2/g
135m2/g
90m2/g

2.0-9.0

60°Ca
40°Cb

HALO LPH-C18

C18

2 µm
2.7 µm

90 Å

-

6.5%

L1

120m2/g
135m2/g

1.0-8.0

90°Ca
40°Cb

HALO® AQ-C18

C18

2 µm
2.7 µm
5 µm

90 Å

+

6.5%
6.7%
5.6%

L1

120m2/g
135m2/g
90m2/g

2.0-9.0

60°Ca
40°Cb

HALO® PCS-C18

positively charged surface

C18

2.7 µm

90 Å

+

7.5%

L1

125 m²/g

2.0-7.0

60°Ca
40°Cb

HALO® C8

C8

2 µm
2.7 µm
5 µm

90 Å

+

4.8%
5.4%
3.7%

L7

120m2/g
135m2/g
90m2/g

2.0-9.0

60°Ca
40°Cb

HALO® Phenyl-Hexyl

Phenylhexyl

2 µm
2.7 µm
5 µm

90 Å

+

6.3%
7.1%
5.2%

L11

120m2/g
135m2/g
90m2/g

2.0-9.0

60°Ca
40°Cb

HALO® Biphenyl

Biphenyl

2.7 µm

90 Å

+

7.0 %

L11

135m2/g

2.0-9.0

60°Ca
40°Cb

HALO® PCS Phenyl-Hexyl

positively charged surface

Phenyl-Hexyl

2.7 µm

90 Å

+

6.1%

L11

125 m²/g

2.0-7.0

60°Ca
40°Cb

HALO® ES-CN

Diisopropylcyanopropyl

2 µm
2.7 µm
5 µm

90 Å

+

3.5%
3.5%
2.5%

L10

120m2/g
135m2/g
90m2/g

1.0-8.0

80°Ca
40°Cb

HALO® PFP

Pentafluorophenylpropyl

2 µm
2.7 µm
5 µm

90 Å

+

5.3%
5.5%
3.9%

L43

120m2/g
135m2/g
90m2/g

2.0-9.0

60°Ca
40°Cb

HALO® RP-Amide

C16 Amide

2 µm
2.7 µm
5 µm

90 Å

+

7.3%
8.2%
5.5%

L60

120m2/g
135m2/g
90m2/g

2.0-9.0

60°Ca
40°Cb

HALO® HILIC

-

2 µm
2.7 µm
5 µm

90 Å

N/A

-

L3

120m2/g
135m2/g
90m2/g

1.0-8.0

60°Ca
40°Cb

HALO® Penta-HILIC

Penta-hydroxy ligand

2 µm
2.7 µm
5 µm

90 Å

-

2.8%
3.2%
2.1%

L95

120m2/g
135m2/g
90m2/g

2.0-9.0

60°Ca
40°Cb

low pH; high pH

HALO® Bioclass Columns

Phase name

Modification

Particle size

Pore size

End-capping

C-content

USP

Surface area

pH range

Tmax

HALO® Protein
ES-C18

C18

2.7 µm
3.4 µm

1000 Å
400 Å

+

1.4%
1.0%

L1

15 m2/g

1.0-8.0

90°Ca
40°Cb

HALO® Protein
C4

C4

2.7 µm
3.4 µm

1000 Å
400 Å

+

0.6%
0.4%

L26

15 m2/g

2.0-9.0

90°Ca
40°Cb

HALO® Protein Diphenyl

Diphenylmethyl

2.7 µm
3.4 µm

1000 Å
400 Å

+

N/A
1.0%

L11

15 m2/g

2.0-9.0

90°Ca
40°Cb

HALO® Peptide ES-C18

C18

2 µm
2.7 µm
5 µm

160 Å

-

4.0%
4.6%
4.0%

L1

65 m2/g
90 m2/g
60 m2/g

1.0-8.0

90°Ca
40°Cb

HALO® PCS-C18

positively charged surface

C18

2.7 µm

160 Å

+

5.1%

L1

125 m²/g

2.0-7.0

60°Ca

40°Cb

HALO® Peptide
ES-CN

Diisopropylcyanopropyl

2.7 µm
5 µm

160 Å

+

2.2%
1.5%

L10

90 m2/g
60 m2/g

1.0-8.0

90°Ca
40°Cb

HALO® Peptides
Phenyl-hexyl

Phenylhexyl

2.7 µm

160 Å

+

4.7%

L11

90 m2/g

2.0-9.0

90°Ca
40°Cb

HALO® Glycan

Penta-hydroxy ligand

2.7 µm

160 Å

-

3.2%

N/A

135 m2/g

2.0-9.0

65°Ca
40°Cb

low pH; high pH

HALO® Enviroclass columns:

Phase name

Modification

Particle size

Pore size

End-capping

C-content

USP

Surface area

pH range

Tmax

HALO® PAH

C18

2.7 µm

90 Å

-

9.9%

N/A

135m2/g

2.0-9.0

60°Ca
40°Cb

HALO® PFAS

C4

2.7 µm

90 Å

+

N/A

N/A

135m2/g

2.0-9.0

60°Ca
40°Cb

HALO® PFAS Delay

Proprietary

2.7 µm

90 Å

+

N/A

N/A

90m2/g

2.0-9.0

60°Ca
40°Cb

low pH; high pH

Applications

HALO® Biphenyl

The HALO® Biphenyl is ready for the challenge of complex samples. Both polar and non-polar pesticides are well resolved with this commonly used test for cannabis.

 

 

ADVANTAGES OF THE NEW HALO® BIPHENYL

  • Improved retention, high sensitivity and higher efficiency without sacrificing robustness or reproducibility
  • Increased selectivity for challenging polar compounds
  • High efficiency leading to sharply resolved peaks with excellent peak shapes

Peak Identities

1 Daminozide  2 Flonicamid  3 Thiamethoxam  4 Imidacloprid  5 Paclobutrazol  6 Fenhexamid  
7 Myclobutanil  8 Bifenazate  9 Dimethomorph Isomer 1  10 Pirotetramat  11 Dimethomorph Isomer 2  
12 Spinosad A  13 Spinosad D  14 Trifloxystrobin  15 Spinetoram  16 Pyrethrin II  17 Piperonyl butoxide  
18 Pyrethrin I  19 Etoxazole  20 Abamectin A  21 Cypermethrin  22 Bifenthrin  23 Acequinocyl

Isomer match

Tocopherols, a form of vitamin E known for its antioxidant properties, contain many isomers that can be easily separated in the baseline on the HALO® C30 compared to a C18 phase due to the phase's shape selectivity.

 

PEAKIDENTITIES

  • 1. δ-tocopherol  2. γ- tocopherol  3. β- tocopherol  4. α- tocophero

TEST CONDITIONS

  • Column: HALO 160 Å C30 and 90 Å C18 , 2.7 µm, 4.6 x 150mm
  • Mobile Phase A: Water
  • Mobile Phase B: Methanol
  • Isocratic: 95% B
  • Flow rate: 1.5 mL/min
  • Temperature: 10°C Injection
  • Injection volume: 1.5 μL
  • Instrument: Agilent 1200 SL
  • Detection: UV 290 nm, PDA

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