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Vitamins
Vitamins are organic compounds that are essential for vital processes and functions in the body. They are essential, i.e. they must be taken in with food and cannot be synthesised in sufficient quantities by the human body itself. A distinction is made between water-soluble and fat-soluble vitamins. Water-soluble vitamins are mainly analysed by liquid chromatography using reversed-phase chromatography or hydrophilic interaction chromatography (HILIC). Fat-soluble vitamins are often analysed using reversed-phase chrom atography or normal-phase chromatography.
Technical Data
1. Water-soluble Vitamins:
Vitamin C (ascorbic acid)
Vitamin B1 (thiamine)
Vitamin B2 (riboflavin)
Vitamin B3 (nicotinic acid; niacin)
Vitamin B5 (pantothenic acid)
Vitamin B6 (pyridoxine, pyridoxal and pyridoxamine)
Vitamin B7 (biotin)
Vitamin B9 (folic acid)
Vitamin B12 (cobalamin)
2. Fat-soluble Vitamins:
[English:]
Vitamin A (retinol)
Vitamin D (colecalciferol)
Vitamin E (tocopherol)
Vitamin K (phylloquinone (K1), menaquinone-n (K2), menadione (K3))
Applications
Water-soluble vitamins Reversed Phase with MZ-Analysentechnik MZ-Aqua Perfect C18
Peak identities
1. thyamine (vitamin B1) 2. ascorbic acid (vitamin C) 3. nicotinic acid (vitamin B) 4. nicotinamide (vitamin B3) 5. pyridoxal (vitamin B6) 6. pyridoxine (vitamin B6) 7. folic acid (vitamin B9) 8. cyanocobalmin (vitamin B12) 9. riboflavin (vitamin B2)
Test conditions
Column: MZ-Aqua Perfect C18 200x4.0mm, 3µm
Mobile phase A: 0.05 M phosphoric acid
Mobile phase B: 0.05 M phosphoric acid in water/acetonitrile 70/30
Gradient:
| Time / min | %A | %B |
| 0 | 100 | 0 |
| 3 | 100 | 0 |
| 6 | 0 | 100 |
| 12 | 0 | 100 |
Flow rate: 1.25 mL/min
Injection volume: 10 µL
Detection: UV, 230 nm
Fat-soluble Vitamins Reversed Phase with GL Sciences Inertsil ODS-4
Peak identities
1. vitamin K3 2. vitamin A 3. vitamin A acetate 4. vitamin D3 5. vitamin E 6. vitamin E acetate 7. vitamin K1 8. α-carotene 9. β-carotene
Test conditions
Column: Inertsil ODS-4 150x4.6mm, 5µm (5020-03945)
Mobile phase A: methanol
Mobile phase B: Ethanol
Isocratic: 75/25 A/B
Flow rate: 1 mL/min
Temperature: 40 °C
Detection: UV, 280 nm
Injection volume: 5 µL
Downloads
Manufacturer overview
MZ-Analysentechnik
Water soluble vitamins
HPLC - Reversed Phase:
- MZ-Aqua Perfect C18, 3µm
- PerfectSil Target ODS-3 HD, 5µm
AMT
Water soluble vitamins
UHPLC - HILIC:
- HALO 2 HILIC, 2µm
Fat soluble vitamins
HPLC - Reversed Phase:
- HALO PFP, 2.7µm
ChromaNik
Water soluble vitamins
HPLC - Reversed Phase:
- Sunniest RP-Aqua, 5µm
- SunShell RP-Aqua, 2.6µm
Fat-soluble vitamins
HPLC - Reversed Phase:
- Sunrise C18-SAC, 3µm
- SunShell C18, 2.6µm
GL Sciences
Water soluble vitamins
HPLC - Reversed Phase:
- InertSustain C18, 5µm
- InertSustain C18, 3µm
- Inertsil ODS-SP, 5µm
- Inertsil NH2, 5µm
Fat-soluble vitamins
HPLC - Reversed Phase:
- Inertsil ODS-4, 5µm
- Inertsil ODS-P, 5µm
- InertSustainSwift C18, 5µm
- GL-Sciences Inertsil NH2 Analysis of Vitamin C Applications
- GL-Sciences Inertsil ODS-4 Analysis of Fat-soluble Vitamins Applications
- GL-Sciences Inertsil ODS-P Analysis of cis-trans Isomers of Vitamin A Applications
- GL-Sciences Inertsil ODS-SP Analysis of Water-soluble Vitamins Applications
- GL-Sciences InertSustain C18 Analysis of Water-soluble Vitamins Applications
- GL-Sciences InertSustainSwift C18 Analysis of Vitamin K Applications
Imtakt
Water-soluble vitamins
HPLC - Normal Phase/IEX
- Unison UK-Amino, 3µm
- Unison UK-Silica
Fat-soluble vitamins
HPLC - Reversed Phase:
- Cadenza CD-C18
- Unison UK-Silica
Macherey-Nagel
Water-soluble vitamins
HPLC - Reversed Phase:
- Nucleoshell RP 18plus, 2.7µm
HPLC - HILIC:
- Nucleodur HILIC, 3µm
Sample preparation:
- Chromabond HILIC
Fat-soluble vitamins
HPLC - Reversed Phase:
- Nucleosil 100 C18, 5µm
- Nucleodur C18 Isis, 5µm
Sample preparation:
- Chromabond XTR
Nouryon - Kromasil
Water soluble vitamins
HPLC - HILIC:
- Kromasil 60 HILIC-D, 5µm
Fat-soluble vitamins
UHPLC - Reversed Phase
- Kromasil EternityXT-1.8-C18, 1.8µm
Shodex
Water soluble vitamins
HPLC - Reversed Phase:
- Asahipak ODP-50 6D, 5µm
Fat-soluble vitamins
HPLC - Normal Phase:
- Silica 5SIL 4D, 5µm
Tosoh
Water soluble vitamins
HPLC - Reversed Phase:
- TSKgel ODS-100V, 5µm
Fat-soluble vitamins
HPLC - Reversed Phase
- TSKgel ODS-100V, 5µm
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