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Vitamins are organic compounds that are essential for vital processes and functions in the body. They are essential, i.e. they must be taken in with food and cannot be synthesised in sufficient quantities by the human body itself. A distinction is made between water-soluble and fat-soluble vitamins. Water-soluble vitamins are mainly analysed by liquid chromatography using reversed-phase chromatography or hydrophilic interaction chromatography (HILIC). Fat-soluble vitamins are often analysed using reversed-phase chrom atography or normal-phase chromatography.
Vitamin C (ascorbic acid)
Vitamin B1 (thiamine)
Vitamin B2 (riboflavin)
Vitamin B3 (nicotinic acid; niacin)
Vitamin B5 (pantothenic acid)
Vitamin B6 (pyridoxine, pyridoxal and pyridoxamine)
Vitamin B7 (biotin)
Vitamin B9 (folic acid)
Vitamin B12 (cobalamin)
[English:]
Vitamin A (retinol)
Vitamin D (colecalciferol)
Vitamin E (tocopherol)
Vitamin K (phylloquinone (K1), menaquinone-n (K2), menadione (K3))
1. thyamine (vitamin B1) 2. ascorbic acid (vitamin C) 3. nicotinic acid (vitamin B) 4. nicotinamide (vitamin B3) 5. pyridoxal (vitamin B6) 6. pyridoxine (vitamin B6) 7. folic acid (vitamin B9) 8. cyanocobalmin (vitamin B12) 9. riboflavin (vitamin B2)
Column: MZ-Aqua Perfect C18 200x4.0mm, 3µm
Mobile phase A: 0.05 M phosphoric acid
Mobile phase B: 0.05 M phosphoric acid in water/acetonitrile 70/30
Gradient:
Time / min | %A | %B |
0 | 100 | 0 |
3 | 100 | 0 |
6 | 0 | 100 |
12 | 0 | 100 |
Flow rate: 1.25 mL/min
Injection volume: 10 µL
Detection: UV, 230 nm
1. vitamin K3 2. vitamin A 3. vitamin A acetate 4. vitamin D3 5. vitamin E 6. vitamin E acetate 7. vitamin K1 8. α-carotene 9. β-carotene
Column: Inertsil ODS-4 150x4.6mm, 5µm (5020-03945)
Mobile phase A: methanol
Mobile phase B: Ethanol
Isocratic: 75/25 A/B
Flow rate: 1 mL/min
Temperature: 40 °C
Detection: UV, 280 nm
Injection volume: 5 µL
Water soluble vitamins
HPLC - Reversed Phase:
Water soluble vitamins
UHPLC - HILIC:
Fat soluble vitamins
HPLC - Reversed Phase:
Water soluble vitamins
HPLC - Reversed Phase:
Fat-soluble vitamins
HPLC - Reversed Phase:
Water soluble vitamins
HPLC - Reversed Phase:
Fat-soluble vitamins
HPLC - Reversed Phase:
Water-soluble vitamins
HPLC - Normal Phase/IEX
Fat-soluble vitamins
HPLC - Reversed Phase:
Water-soluble vitamins
HPLC - Reversed Phase:
HPLC - HILIC:
Sample preparation:
Fat-soluble vitamins
HPLC - Reversed Phase:
Sample preparation:
Water soluble vitamins
HPLC - HILIC:
Fat-soluble vitamins
UHPLC - Reversed Phase
Water soluble vitamins
HPLC - Reversed Phase:
Fat-soluble vitamins
HPLC - Normal Phase:
Water soluble vitamins
HPLC - Reversed Phase:
Fat-soluble vitamins
HPLC - Reversed Phase
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