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Peptides
Peptides are short chains of amino acids that are linked together by peptide bonds and play a crucial role in many biological processes. They act as signalling molecules, hormones or enzymes and are often involved in the regulation of cell functions. Due to their diverse biological activities, peptides are of great importance in research and medicine, for example in the development of therapeutics and diagnostics.
High-performance liquid chromatography (HPLC) is a key method for analysing peptides. It enables the precise separation, identification and quantification of peptide structures, which is essential for quality control in peptide synthesis and for the characterisation of biological samples. HPLC offers high resolution and sensitivity, which is particularly important for complex peptide mixtures to ensure purity, stability and functionality.
Technical Data
Tryptic digestion (Tryptic Digest)
Tryptic digestion is a widely used process for splitting proteins into smaller peptides. It uses the enzyme trypsin, which specifically cuts the peptide bonds after the amino acids lysine and arginine. This method is particularly useful for breaking down large proteins into manageable peptide fragments, which can then be further analysed. It plays a central role in proteomics, the study of proteins and their functions (bottom-up method).
After tryptic digestion, the resulting peptide fragments are often analysed using high-performance liquid chromatography (HPLC). HPLC makes it possible to separate the different peptides based on their size, polarity or charge. By combining HPLC with other analytical methods, such as mass spectrometry (HPLC-MS), proteins can be characterised in detail. This is important for the identification of proteins, the investigation of post-translational modifications and for quantitative analyses in biological samples.
Downloads
AMT
HPLC - Reversed Phase:
- HALO Peptide ES-C18
- HALO Peptide ES-CN
- HALO Peptides Phenyl-Hexyl
ChromaNik
HPLC - Reversed Phase:
- SunShell C18-16, 2.6µm
- SunShell C18-WP, 2.6µm
- SunShell RP-AQUA, 2.6µm
GL Sciences
Sample preparation - Tryptic Digest:
- MonoTip Trypsin
Sample preparation - Desalting and enrichment:
- MonoTip C18
Sample preparation - Phosphopeptide enrichment:
- Titansphere
- Titansphere Phos-TiO Kit
- Titansphere Phos-TiO MP Kit
- Titansphere Phos-TiO for large samples
Sample preparation - Desalting of phosphopeptides:
- GL-Tip SDB
- GL-Tip GC
- MonoTip TiO
Sample preparation - removal of phosphopeptides:
- MonoSpin Phospholipid
Sample preparation - purification and enrichment of small samples:
- MonoSpin Series
Sample preparation - peptide fractionation:
- GL-Tip SCX
- GL-Tip SDB-SCX
HPLC - Reversed Phase:
- ProteoSil C18
- ProteoSil C4
- Inertsil WP300 C18
HPLC - HILIC:
- ProteoSil HILIC
HPLC - SEC:
- ProteoSil SEC
Imtakt
HPLC - Reversed Phase:
- Cadenza CD-C18, 3µm
- Unison UK-C18, 3µm
- Cadenza CL-C18, 3µm
- Unison UK-Amino, 3µm
- Cadenza HS-C18, 3µm
- Cadenza CW-C18, 3µm
- Unison UK-Phenyl, 3µm
- Unison UK-C8, 3µm
- Unison US-C18, 5µm
UHPLC - Reversed Phase:
- Presto FF-C18, 2µm
HPLC - Normal Phase:
- Unison UK-Silica, 3µm
- Unison UK-Amino, 3µm
HPLC - Mixed-Mode:
- Scherzo SM-C18, 3µm
Macherey-Nagel
HPLC - Reversed Phase:
- Nucleosil C18, 5µm
- Nucleodur C18 ec, 5µm
- Nucleogel RP, 5µm
- Nucleodur C4 ec, 5µm
- Macherey-Nagel Nucleodur C4 ec Comparing the Separation of Peptides
- Macherey-Nagel Nucleodur C18 ec Biosynthesis and Stability of the Lasso Peptide Capistruin
- Macherey-Nagel Nucleogel RP Peptide Standards
- Macherey-Nagel Nucleogel RP Reversed Phase Peptide Analysis
- Macherey-Nagel Nucleosil C18 Analysis and Purification of Toxic Peptides from Cyanobacteria
- Macherey-Nagel Nucleosil C18 "Peptaibols" and other aib-containing Peptides of Fungal Origin
- Macherey-Nagel Nucleosil C18 Separation of Peptides derived from Sodium Dodecyl Sulfate-solubilized Proteins
- Macherey-Nagel Nucleosil C18 Unexpected Elution behaviour of Peptides
- Macherey-Nagel Nucleosil C18 Amino Acid Analysis of Peptide Hydrolyzates by OPA Derivatives
- Macherey-Nagel Nucleodur C18 ec Tryptic Digest of Cytochrome C
- Macherey-Nagel Nucleosil C18 Separation of a Tryptic Digest of Human Growth Hormone
- Macherey-Nagel Nucleosil C18 True versus Immunoreactive Angiotensin (II) in Human Plasma
Nacalai Tesque
HPLC - Reversed Phase:
- Cosmosil C18-AR-300, 5µm
Osaka Soda
HPLC - Reversed Phase:
- Proteonavi, 5µm
- Capcell PAK C18 AG120, 5µm
- Capcell CORE ADME, 2.7µm
- Capcell PAK NH2 UG80, 5µm
UHPLC - Reversed Phase:
- Capcell PAK ADME-HR, 2µm
- Capcell PAK C18 MGII, 2µm
HPLC - IEX:
- Capcell PAK SCX UG80, 5µm
- Osaka Soda Capcell Core ADME Peptides
- Osaka Soda Capcell PAK ADME-HR Peptides
- Osaka Soda Capcell PAK C18 AG120 Analysis of Peptides with basic Mobile Phases
- Osaka Soda Capcell PAK C18 AG120 Peptide-type Antibiotics
- Osaka Soda Capcell PAK C18 MGII Peptides
- Osaka Soda Capcell PAK NH2 UG80 Imidazole Dipeptides
- Osaka Soda Capcell PAK SCX UG80 Imidazole Dipeptides
- Osaka Soda Proteonavi Cyclic Peptides
- Osaka Soda Proteonavi Cyclic Peptides (1)
- Osaka Soda Proteonavi Peptides
- Osaka Soda Proteonavi Peptides (1)
- Osaka Soda Proteonavi Peptides (2)
Sepax
HPLC - Reversed Phase:
- Bio-C18, 5µm
HPLC - SEC:
- Zenix SEC-150, 3µm
- Zenix SEC-80, 3µm
- Zenix-C SEC-80, 3µm
- Zenix SEC-100, 3µm
HPLC - IEX:
- Proteomix SCX, 5µm
UHPLC - IEX:
- Proteomix SCX, 1.7µm
- Sepax Bio-C18 Analysis of Microcystin
- Sepax Bio-C18 Human Serum Albumin Tryptic Digest Separation
- Sepax Proteomix SCX Exenatide Acetate Analysis
- Sepax Proteomix SCX Analysis of Peptide Digest
- Sepax Proteomics SCX Gradient Comparisons for the Analysis of Peptides
- Sepax Proteomix SCX Separation of Peptides
- Sepax Zenix SEC-80 Analysis of E.coli Tryptic Digest
- Sepax Zenix SEC-80 Effect of Acetonitril Mobile Phase Concentration
- Sepax Zenix SEC-80 Peptide Separations using Size Exclusion Chromatography
- Sepax Zenix SEC-80 Peptide Separations from 13.7kD to 120Da
- Sepax Zenix SEC-80 Analysis of Four Peptide Mixture from 1kD to 6kD
- Sepax Zenix SEC-80 Zenix-C SEC-80 Peptide Separation
- Sepax Zenix SEC-80 Zenix-C SEC-300 Pegylated Exenatide
- Sepax Zenix SEC-100 Analysis of Peptide Mixture
- Sepax Zenix SEC-150 Analysis of 5kD and 8kD Peptides
Shodex
HPLC - Reversed Phase:
- Asahipak ODP-50 6D
- RSpak DE-613
HPLC - Mixed-Mode:
- Asahipak GS-320 7G
HPLC - HILIC:
- HILICpak VC-50 2D
- Shodex Asahipak C8P-50 4D High Molecular Weight Peptides Applications
- Shodex Asahipak GS-320 7G Peptides in Extract from Waste Water Sludge
- Shodex Asahipak ODP-50 6D Basic Peptides
- Shodex Asahipak ODP-50 6D Oligopeptides (1)
- Shodex Asahipak ODP-50 6D Analysis of Oligopeptides with Acidic and Alkaline Eluent
- Shodex Asahipak ODP-50 6D Oligopeptides (2)
- Shodex Asahipak ODP-50 6D Opioid Peptides (1)
- Shodex Asahipak ODP-50 6D Opioid Peptides (2)
- Shodex HILICpak VC-50 2D LC/MS Analysis of Low Molecular Weight Peptides
- Shodex RSpak DE-613 Dipeptides
Thermo
UHPLC - Reversed Phase:
- Hypersil GOLD Peptide, 1.9µm
- Hypersil GOLD VANQUISH C18
HPLC - Reversed Phase:
- Accucore 150-C18, 2.6µm
- Accucore 150-C4, 2.6µm
- PepMap (Bottom-Up)
- µPAC (Bottom-Up)
- PepSwift
HPLC - HIC:
- ProPac HIC
HPLC - metal affinity:
- ProPac IMAC-10
Sample preparation - Digestion:
- SMART Digest
- Thermo Hypersil GOLD Peptide Brochure
- Thermo Accucore HPLC Columns for Biomolecule Separations HPLC Life Science Brochure
- Thermo BioLC Columns Consumables HPLC Life Science Brochure 2022-23
- Thermo PepSwift ProSwift HPLC Brochure
- Thermo ProPac IMAC 10 HPLC Life Science Brochure
- Thermo ProPac HIC 10 HPLC Life Science Brochure
- Thermo Smart Digest Protein Digestion Life Science Brochure
Tosoh
HPLC - SEC:
- TSKgel G2500PWXL, 7µm
- TSKgel G2000SWXL, 5µm
- TSKgel G3000SW, 10µm
HPLC - Reversed Phase:
- TSKgel ODS-100V, 5µm
- TSKgel ODS-80TS, 5µm
UHPLC - Reversed Phase:
- TSKgel Octadecyl-NPR, 2.5µm
- TSKgel Super-ODS, 2.3µm
- Tosoh TSKgel Amide-80 Separation of Peptides by HILIC Applications
- Tosoh TSKgel G2000SWXL Separation of Polypeptides
- Tosoh TSKgel G2500PWXL, G2000SWXL Separation of Peptides Mixture
- Tosoh TSKgel G2500PWXL, G2000SWXL Separation of Peptides Mixture (1)
- Tosoh TSKgel G3000SW Separation of Polypeptides
- Tosoh TSKgel Octadecyl-NPR Separation of Standard Peptides
- Tosoh TSKgel ODS-100V Separation of Histidine-containing Dipeptides
- Tosoh TSKgel SuperSW2000 Separation of Mixture of Proteins and Peptides Applications
- Tosoh TSKgel Octadecyl-NPR Separation of Trypsin Digest of Cytochrome C
- Tosoh TSKgel ODS-80TS Separation of Tryptic Digest of beta-Lactoglobulin
- Tosoh TSKgel Super-ODS Separation of Tryptic Digest of beta-Lactoglobulin
Waters
UHPLC - Reversed Phase:
- Acquity UPLC Peptide CSH C18, 1.7µm
- Acquity Premier Peptide CSH C18, 1.7µm
- Acquity UPLC Peptide HSS T3, 1.8µm
- Acquity Premier Peptide HSS T3, 1.8µm
- Acquity UPLC Peptide BEH C18, 1.7µm
- Acquity Premier Peptide BEH C18, 1.7µm
- XSelect Peptide CSH C18, 2.5µm
- XSelect Premier Peptide CSH C18, 2.5µm
- XSelect Peptide HSS T3, 2.5µm
- XSelect Premier Peptide HSS T3, 2.5µm
- nanoEase MZ Peptide BEH C18, 1.7µm
- XBridge Premier Peptide BEH C18, 2.5µm
HPLC - Reversed Phase:
- XBridge Peptide BEH C18, 3.5µm
- BioSuite C18
- Delata-Pak
HPLC - IEX:
- Protein-Pak HR Q
- Protein-Pak HR DEAE
- Protein-Pak HR SP
- Protein-Pak HR CM
- BioSuite SP
- BioSuite CM
- BioSuite Q
- BioSuite DEAE
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