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Glyphosat
Glyphosate - Analysis of a Controversial Plant Protection Product
Glyphosate is an herbicide that has been on the market since the 1970s and has since become the most commonly used weedkiller. The use of glyphosate is highly controversial among experts, users and consumers, as it is suspected of being carcinogenic.
Various liquid chromatography separation techniques can be used to analyse glyphosate and its metabolites (e.g. aminomethylphosphoric acid - AMPA). These include ion chromatography, hydrophilic interaction chromatography (HILIC) and reversed-phase chromatography. Due to the high polarity of glyphosate and its UV inactivity, the amino nitrogen is often derivatised with an Fmoc protecting group during sample preparation, which facilitates the separation and detection of the resulting substances.
Technical Data
1. Hydrophilic Interaction Chromatography (HILIC)
Shodex™ HILICpak VT-50 2D (5 µm, 2.0x150 mm, P/N: F7630400)
Analytes: Glyphosate, Glufosinate, AMPA, MPPA
Application
MicroSolv™ Cogent Diamond Hydride (4 µm, 2.1x150 mm, P/N: 70000-15P-2)
Analyte: Glyphosate
2. Ion or Ion Exchange Chromatography
Shodex™ IC SI-90 4E (9 µm, 4.0x250 mm, P/N: F6995244)
Analytes: glyphosates, glufosinates
Thermo Scientific™ Dionex™ IonPac™ AS24 (7 µm, 2.0 x 250 mm, P/N: 064153)
Analytes: Glyphosate, Glufosinate, AMPA, Ethephon, Fosetyl-Al, MPPA, Phosphonic acid, HEPA, N-Ac-AMPA, N-Ac-Glufosinate
3. Reversed-Phase Chromatography
GL Sciences™ Inertsil ODS-4 (5 µm, 4.6x150 mm, P/N: 5020-03945)
Analyte: Glyphosate
Machery-Nagel Nucleoshell RP-18 (2.7 µm, 2.0x100 mm, P/N: 763134.20)
Analytes: Fmoc-Glyphosate, Fmoc-Glufosinate, Fmoc-AMPA
GL Sciences™ InertSustainSwift C18 (3 µm, 2.1x150 mm, P/N: 5020-88128)
Analytes: Fmoc-Glyphosate, Fmoc-Glufosinate, Fmoc-AMPA
Thermo Scientific™ Hypercarb™ (5 µm, 2.1x50 mm, P/N: 35005-052130)
Analytes: Glyphosate, AMPA
Application
Imtakt™ Scherzo SM-C18 (Mixed-Mode, 3 µm, 3.0x250 mm, P/N: SM036)
Analytes: Glyphosate, Glufosinate, Fosetyl, Paraquat (2Cl-), Diquat (2Br-)
ACE® Excel 3 Super C18 (3 µm, 2.1x150 mm, P/N: EXL-1111-1502U)
Analytes: Fmoc-Glyphosate, Fmoc-Glufosinate, Fmoc-AMPA
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