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Fipronil
Fipronil is a pesticide that is used in many countries around the world. It is effective against a large number of agricultural pests, but also has a harmful effect on humans above certain levels. It is suspected of being carcinogenic. The use of fipronil in food-producing animals is prohibited.
Chemically speaking, fipronil belongs to the phenylpyrazole group. It is practically insoluble in water, but shows good solubility in polar organic solvents such as acetone or ethyl acetate. Fipronil is an optically active substance, i.e. fipronil is present in two mutually enantiomeric forms. These can be separated using chiral HPLC. However, fipronil is used as a racemate (as a mixture of enantiomers in a 1:1 ratio).
Fipronil is often analysed using liquid or gas chromatography coupled to a mass spectrometer (LC-MS or GC-MS). The QuEChERS method is often used for sample preparation.
Below you will find some column recommendations for the analysis of fipronil with corresponding application examples:
Technical Data
Columns for Liquid Chromatography
| Column Name | Particle Size | Pore Size | Column Size | Part-Number |
| Kromasil Eternity C18 | 2.5 µm | 100 Å | 4.6x50 mm | EH2CLA05 |
| Imtakt Unison UK C18 Imtakt Cadenza CD-C18 | 3 µm | 130 Å 120 Å | 2.0x50 mm | UK022T CD022T |
| Macherey-Nagel Bluebird RP 18 | 2.7 µm | 90 Å | 2.0x100 mm | 763434.20 |
| Thermo Scientific Accucore aQ | 2.6 µm | 80 Å | 2.1x100 mm | 17326-102130 |
| Thermo Scientific Hypersil GOLD aQ | 1.9 µm | 175 Å | 2.1x100 mm | 25302-102130 |
Applications
- Kromasil Eternity 2.5-C18 Fipronil Applications
- Imtakt Unison UK-C18 Cadenza CD-C18 Insecticides Applications
- Macherey-Nagel NUCLEOSHELL Bluebird RP 18 Fipronil in Eggs Applications
- Thermo Accucore AQ Pesticide Residues Screening Analysis in Tea & Honey HPLC Applications
- LC-MS Applications for Food Safety Analysis
Columns for Liquid Chromatography with Chiral Stationary Phases
| Column Name | Particle Size | Pore Size | Column Size | Part-Number |
| Astec® (R,R) P-CAP™ | 5 µm | 200 Å | 4.6x250 mm | 31024AST |
| Chiralpak IB | 5 µm | N/A | 4.6x250 mm | 81325 |
Columns for Gas Chromatography
| Column Name | Particle Size/ | Column Size | Part-Number |
| Thermo Scientific TraceGOLD TG-5SilMS GC | 0.25 µm | 0.25mm x 30m | 26096-1425 |
| GL-Sciences InertCap Pesticides | 0.20 µm | 0.25mm x 30m | 1010-15141 |
| Supelco SLB®-5ms | 0.10 µm | 0.10mm x 10m | 28465-U |
| Supelco SLB®-5ms | 0.25 µm | 0.25mm x 30m | 28471-U |
Applications
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