Acrylamide

Acrylamide is formed when food is heated to high temperatures, e.g. when frying or deep-frying. The amino acid asparagine reacts with reducing sugars such as glucose or fructose (Figure 1). Cereal products (cereals, baked goods, etc.), potato products (chips, crisps) and coffee are particularly affected.

Figure 1: Formation of acrylamide.

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Technical Data

Animal experiments have shown that acrylamide is carcinogenic and damages the genome. It has therefore been categorised as "probably carcinogenic to humans". However, there are no binding limits for acrylamide in food (in the EU). Only guide values and minimisation measures were defined in 2013 and set out in a regulation at the end of 2017. The guideline values were lowered again. This guideline value is determined by the acrylamide value, which 90% of the samples fell below. There may therefore be further lower guideline values in the future. As a result, analysing acrylamide in food has also become essential.

Based on these developments, many manufacturers have published applications for the determination of acrylamide. These range from reversed-phase chromatography, normal-phase chromatography and HILIC to gas chromatography.

Reversed Phase Chromatography

GL Sciences

GL Sciences Inertsil ODS-4 Analysis of Acrylamide, Acrylonitrile and Organic Acids HPLC Application

Imtakt

Macherey-Nagel

Osaka-Soda

Osaka-Soda CAPCELL PAK C18 AQ S3 Acrylamide Applications

Perkin-Elmer

Perkin-Elmer Altus UPLC BEH C18 Acrylamid Analysis in Waste Water Applications

Restek

Shodex

Shodex RSpak DE-413 Amides Applications

Sielc

Sielc Primesep 200 Separation of Acrylamide and Melamine Applications

Supelco

Thermo Fisher

Thermo Hypercarb MS Assay for the Determination of Acrylamide From Foodstuffs HPLC Applications

Tosoh

Tosoh TSKgel ODS-100V Separation of Acrylamide by RPC Applications

Waters

Welch

welch_ultisil_xb-c18_determination-of-acrylamide-in-food_hplc_applications.pdf

ZirChrom

ZirChrom ZirChrom-CARB Fast Separation of Acrylamide Monomer from Acrylic Acid Applications

HILIC

Macherey-Nagel

Osaka-Soda

Osaka-Soda CAPCELL CORE PC S2.7 Acrylamide Applications

Tosoh

Tosoh TSKgel Amide-80 Separation of Acrylamide by HILIC Applications

Waters

Normal Phase Chromatography

Imtakt

Ion Chromatography

Thermo Fisher

Thermo Dionex IonPac ICE AS1 Fast Determination of Acrylamide in Food Samples HPLC Applications

Gas Chromatography

GL Sciences

GL Sciences InertCap-Wax Acrylonitrile, Acrylamide, Propionamide GC Application

Restek

Shimadzu

Shimdazu CP-Sil 24 CB Analysis of Acrylamide in Processed Foods Applications

Thermo Fisher

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