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Cells
Cells are a crucial component in chromatography and are of great importance for the detection and analysis of samples. These special chambers, also known as flow cells or detector cells, are integral parts of detectors used in chromatographic systems to measure and quantify the separate components of a sample.
In liquid chromatography (LC), cells are often part of UV/VIS detectors, fluorescence detectors or other specialised detection methods. The sample flowing through the chromatographic column is passed through the cell where it interacts with a light beam or other detection medium. The cell allows the detector to measure changes in light absorption, fluorescence or other physical properties that provide information about the concentration and identity of the sample components.
Cells are available in various designs and materials to meet different analytical requirements. They are usually made of quartz glass or special plastics that are chemically inert and offer high transparency for the respective detection range. The choice of cell material depends on the type of analysis and the solvents used in order to avoid interference and contamination.
An important parameter of cells is the flow volume, which determines the amount of sample contained in the cell. Small volume cells are advantageous for applications where only small amounts of sample are available or high sensitivity is required. Larger cells are used when larger sample volumes are analysed or higher signal strength is required.
Proper cell maintenance and cleaning is critical to maintaining the accuracy and sensitivity of chromatographic analyses. Contamination or residues in the cells can lead to signal interference and inaccurate results. Regular inspection and cleaning are therefore necessary to ensure the performance of the cells.
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