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Capillaries are an indispensable element in chromatography and play a central role in the separation and analysis of samples. These thin tubes, which are typically made of stainless steel or special plastics, are responsible for transporting the samples through the chromatographic system.
In chromatography, capillaries are used to connect individual components such as injectors, pumps and detectors. The capillaries in HPLC must withstand high pressures and be chemically inert to prevent contamination of the sample and ensure accurate results.
Capillaries are available in different diameters and lengths to meet the specific requirements of each chromatographic method. The choice of the correct capillary depends on several factors, including the type of sample, the physico-chemical properties of the substances to be separated and the operating conditions of the chromatographic system.
An important aspect in the use of capillaries is the prevention of clogging and leakage. Regular maintenance and cleaning of the capillaries are necessary to ensure uniform and undisturbed sample transfer. High quality capillaries have precisely manufactured internal surfaces that minimise contamination and ensure consistent performance.