Standards
Standards are indispensable components in chromatography that contribute significantly to the accuracy, reproducibility and validation of analyses. They serve as reference substances that can be used to determine the concentration and identity of the analytes in the samples. By using standards, laboratories can ensure that their analyses are reliable and consistent.
Standards fulfil several important functions in chromatographic analysis:
- Calibration: standards are used to create calibration curves that represent the relationship between the concentration of an analyte and the detector signal. These curves are necessary to accurately determine the concentrations of analytes in unknown samples.
- Quality control: Standards help to monitor the performance of the chromatographic system. Regular analyses of standards make it possible to check the accuracy and precision of the system and ensure that it is working properly.
- Validation: Standards are used to validate analytical methods. They help to determine parameters such as accuracy, precision, limit of detection and limit of quantification and confirm that the method is fit for purpose.
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Technical Data
There are different types of standards that are used depending on the application and specific requirements:
- Calibration standards: These standards are used to create calibration curves. They consist of known concentrations of an analyte and are used to determine the linear or non-linear relationship between signal and concentration.
- Internal standards: Internal standards are compounds that are added to samples in known amounts. They are used as a reference to compensate for variations in the injection and the analytical procedure and to improve the accuracy of the results.
- Quality control standards: These standards are analysed regularly to monitor the performance of the chromatographic system. They help to identify systematic errors and ensure that the analyses are reliable and reproducible.
- Reference materials: Reference materials are high purity substances that are often provided by recognised organisations. They serve as a primary reference for the validation and calibration of analytical methods.
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