Tosoh Bioscience / TSKgel

TSKgel

TSKgel columns, manufactured by Tosoh Bioscience incorporate an extensive range of columns with either silica or polymer packings. Both high and low pressure TSKgel columns are available covering all modes of liquid chromatography, including reversed-phase, ion exchange, ion chromatography, size exclusion, affinity, hydrophobic interaction (HIC) and hydrophilic interaction (HILIC). Column dimensions from analytical to preparative scale are available.

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TSKgel G2000PW, 12 µm, 125 Å 600 mm x 7.5 mm

TSH-05105

Product Type: SEC/GPC Columns Base Material: Hydroxylated polymethacrylate Delivery Time: approx. 4 days Column Type: Analytical Column Manufacturer: Tosoh Hardware Type: Ready-to-use Column Matrix: Polymer Internal Diameter: 7.5 mm Length: 600 mm Particle Morphology: spherical pH Minimum: 2.0 pH Maximum: 12.0 Pressure Stability: 40 bar Pore Size: 125 Å Technology: HPLC

TSKgel G4000SW, 13 µm, 450 Å 600 mm x 7.5 mm

TSH-05104

Product Type: SEC/GPC Columns Delivery Time: approx. 4 days Column Type: Analytical Column Manufacturer: Tosoh Hardware Type: Ready-to-use Column Matrix: Silica Internal Diameter: 7.5 mm Length: 600 mm Pressure Stability: 30 bar Pore Size: 450 Å Technology: HPLC

TSKgel G3000SW, 10 µm, 250 Å 600 mm x 7.5 mm

TSH-05103

TSKgel G2000SW, 10 µm, 125 Å 600 mm x 7.5 mm

TSH-05102

TSKgel Replacement 0.5 µm Frits, for P/N 03410

TSH-03411

Product Type: HPLC Replacement Frits Delivery Time: approx. 4 days Manufacturer: Tosoh

TSKgel Pre-Column Filter with 0.5 µm Frit

TSH-03410

Product Type: Guard Column Filter Delivery Time: approx. 4 days Manufacturer: Tosoh

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Technical Data

Reversed-Phase Chromatography (RPC)

TSKgel RPC columns are based on high-purity, spherical silica gel. TSKgel ODS-100V is a proprietary monomeric bonded phase, provides complete wetting and retention stability in 100% aqueous mobile phases.

Phase-Name	Modification	Particle Size	Pore Size	End-capping	C-Content	USP	Surface	pH-Range	Tmax
TSKgel ODS-100V	C₁₈	3, 5 µm	100 Å	+	15%	L1	450 m²/g	2.0‒7.5	N/A
TSKgel ODS-100Z	C₁₈	3, 5 µm	100 Å	+	20%	L1	450 m²/g	2.0‒7.5	N/A
TSKgel OligoDNA RP	C₁₈	5 µm	250 Å	-	10%	L1	N/A	2.0‒7.5	N/A
TSK ODS-140HTP	C₁₈	2.3 µm	140 Å	+	8%	L1	N/A	2.0‒7.5	N/A
TSKgel Super-ODS	C₁₈	2.3 µm	110 Å	+	8%	L1	N/A	2.0‒7.5	N/A
TSKgel ODS 80TS	C₁₈	5, 10 µm	80 Å	+	15%	L1	N/A	2.0‒7.5	N/A
TSKgel ODS 80TM	C₁₈	5, 10 µm	80 Å	+	15%	L1	N/A	2.0‒7.5	N/A
TSKgel ODS-120A	C₁₈	5, 10 µm	120 Å	-	20%	L1	N/A	2.0‒7.5	N/A
TSKgel ODS-120T	C₁₈	5, 10 µm	120 Å	+	22%	L1	N/A	2.0‒7.5	N/A
TSKgel Super-Octyl	C₈	2.3 µm	110 Å	+	5%	L7	N/A	2.0‒7.5	N/A
TSKgel Octyl 80TS	C₈	5 µm	80 Å	+	11%	L7	N/A	2.0‒7.5	N/A
TSKgel Protein C₄-300	C4	3 µm	300 Å	+	3%	L26	100 m²/g	2.0‒7.5	N/A
TSKgel TMS 250	C1	10 µm	250 Å	+	5%	L13	N/A	1.5‒7.5	N/A
TSKgel Super-Phenyl	Phenyl	2.3 µm	110 Å	+	3%	L11	N/A	2.0‒7.5	N/A
TSKgel CN 80TS	Cyano	5 µm	80 Å	+	8%	L10	N/A	2.0‒7.5	N/A

Polymer-based TSKgel RPC columns are based on polymethacrylate and are chemically stable at pH=2-12, allowing operation at basic pH where silica-based columns have limited stability. Polymer-based columns can be cleaned and impurities removed by using either strong acid or base.

Phase-Name	Modification	Particle Size	Pore Size	End-capping	C-Content	USP	Surface	pH-Range	Tmax
TSKgel Octadecyl-NPR	C₁₈	2.5 µm	Non-porous	-	N/A	N/A	N/A	2.0‒12.0	N/A
TSKgel Octadecyl-2PW	C₁₈	5 µm	125 Å	-	N/A	N/A	N/A	2.0‒12.0	N/A
TSKgel Octadecyl-4PW	C₁₈	7, 13 µm	500 Å	-	N/A	N/A	N/A	2.0‒12.0	N/A
TSKgel Phenyl-5PW	Phenyl	10, 13 µm	1000 Å	-	N/A	N/A	N/A	2.0‒12.0	N/A

Size Exclusion Chromatography (SEC)

TSKgel SW-Type columns are based on silica gel and are well-suited for the analysis of proteins and nucleic acids using aqueous buffer as mobile phase. The TSKgel SW mAb columns within the TSKgel SW series are specifically designed for the analysis of monoclonal antibodies.

Phase-Name	Modification	Particle Size	Pore Size	pH-Range	T_max	Exclusion Limit (Globular Proteins)	Exclusion Limit (Dextrans)
TSKgel G2000SW	Diol	10, 13 µm	125 Å	2.5‒7.5	30 °C	5.000 ‒ 150.000 Da	1.000 ‒ 30.000 Da
TSKgel G3000SW	Diol	10, 13 µm	250 Å	2.5‒7.5	30 °C	10.000 ‒ 500.000 Da	2.000 ‒ 70.000 Da
TSKgel G4000SW	Diol	13, 17 µm	450 Å	2.5‒7.5	30 °C	20.000 ‒ 7.000.000 Da	4.000 ‒ 500.000Da
TSKgel G2000SW_XL	Diol	5 µm	125 Å	2.5‒7.5	30 °C	5.000 ‒ 150.000 Da	1.000 ‒ 30.000 Da
TSKgel G3000SW_XL	Diol	5 µm	250 Å	2.5‒7.5	30 °C	10.000 ‒ 500.000 Da	2.000 ‒ 70.000 Da
TSKgel G4000SW_XL	Diol	8 µm	450 Å	2.5‒7.5	30 °C	20.000 ‒ 7.000.000 Da	4.000 ‒ 500.000Da
TSKgel BioAssist G2SW_XL*	Diol	5 µm	125 Å	2.5‒7.5	30 °C	5.000 ‒ 150.000 Da	1.000 ‒ 30.000 Da
TSKgel BioAssist G3SW_XL*	Diol	5 µm	250 Å	2.5‒7.5	30 °C	10.000 ‒ 500.000 Da	2.000 ‒ 70.000 Da
TSKgel BioAssist G4SW_XL*	Diol	8 µm	450 Å	2.5‒7.5	30 °C	20.000 ‒ 7.000.000 Da	4.000 ‒ 500.000Da
TSKgel QC-PAK GFC 200	Diol	5 µm	125 Å	2.5‒7.5	30 °C	5.000 ‒ 150.000 Da	1.000 ‒ 30.000 Da
TSKgel QC-PAK GFC 300	Diol	5 µm	250 Å	2.5‒7.5	30 °C	10.000 ‒ 500.000 Da	2.000 ‒ 70.000 Da
TSKgel SuperSW2000	Diol	4 µm	125 Å	2.5‒7.5	30 °C	5.000 ‒ 150.000 Da	1.000 ‒ 30.000 Da
TSKgel SuperSW3000	Diol	4 µm	250 Å	2.5‒7.5	30 °C	10.000 ‒ 500.000 Da	2.000 ‒ 70.000 Da
TSKgel SuperSW mAb HR	Diol	4 µm	250 Å	2.5‒7.5	30 °C	10.000 ‒ 500.000 Da	N/A
TSKgel SuperSW mAb HTP	Diol	4 µm	250 Å	2.5‒7.5	30 °C	10.000 ‒ 500.000 Da	N/A
TSKgel UltraSW Aggregate	Diol	3 µm	300 Å	2.5‒7.5	30 °C	10.000 ‒ 2.000.000 Da	N/A
TSKgel UP-SW3000	Diol	2 µm	250 Å	2.5‒7.5	30 °C	10.000 ‒ 500.000 Da	N/A

* PEEK-Column-Hardware

TSKgel PW-Type columns are based on hydrophilic, rigid, spherical, porous methacrylate beads. They are commonly used for the separation of synthetic polymers, oligosaccharides, nucleic acids and small viruses using aqueous buffer or salt solutions as mobile phase, with up to 50% organic solvent.

Phase-Name	Modification	Particle Size	Pore Size	pH-range	T_max	Exclusion Limit (Globular Proteins)	Exclusion Limit (Dextrans)
TSKgel G2000PW	-	12 µm	125 Å	2.0‒12.0	80 °C	< 2.000 Da	N/A
TSKgel G2500PW	-	12, 17 µm	< 200 Å	2.0‒12.0	80 °C	< 3.000 Da	< 3.000 Da
TSKgel G3000PW	-	12, 17 µm	200 Å	2.0‒12.0	80 °C	< 50.000 Da	< 60.000 Da
TSKgel G4000PW	-	17 µm	500 Å	2.0‒12.0	80 °C	< 300.000 Da	1.000 ‒ 700.000 Da
TSKgel G5000PW	-	17 µm	1000 Å	2.0‒12.0	80 °C	< 1.000.000 Da	50.000 ‒ 2.500.000 Da
TSKgel G6000PW	-	17 µm	> 1000 Å	2.0‒12.0	80 °C	< 8.000.000 Da	500.000 ‒ 50.000.000 Da
TSKgel GMPW	-	17 µm	< 100‒1000 Å	2.0‒12.0	80 °C	500 ‒ 8.000.000 Da	< 50.000.000 Da
TSKgel BioAssist G6PW	-	17 µm	> 1000 Å	2.0‒12.0	80 °C	< 8.000.000 Da	500.000 ‒ 50.000.000 Da
TSKgel G2500PW_XL	-	7 µm	< 200 Å	2.0‒12.0	80 °C	N/A	< 3.000 Da
TSKgel G3000PW_XL	-	7 µm	200 Å	2.0‒12.0	80 °C	< 50.000 Da	< 60.000 Da
TSKgel G4000PW_XL	-	10 µm	< 500 Å	2.0‒12.0	80 °C	< 300.000 Da	1.000 ‒ 700.000 Da
TSKgel G5000PW_XL	-	10 µm	1000 Å	2.0‒12.0	80 °C	< 1.000.000 Da	50.000 ‒ 2.500.000 Da
TSKgel G6000PW_XL	-	13 µm	> 1000 Å	2.0‒12.0	80 °C	< 8.000.000 Da	500.000 ‒ 50.000.000 Da
TSKgel G-DANN-PW	-	10 µm	> 1000 Å	2.0‒12.0	50 °C	< 8.000.000 Da	< 50.000.000 Da
TSKgel GMPW_XL	-	13 µm	100‒1000 Å	2.0‒12.0	80 °C	500 ‒ 8.000.000 Da	< 50.000.000 Da
TSKgel G-Oligo-PW	-	7 µm	125 Å	2.0‒12.0	80 °C	0 < 3.000 Da	N/A
TSKgel SuperMultipore PW-N	-	4 µm	N/A	2.0‒12.0	80 °C	300 ‒ 50.000 Da	N/A
TSKgel SuperMultipore PW-M	-	5 µm	N/A	2.0‒12.0	80 °C	500 ‒ 1.000.000 Da	N/A
TSKgel SuperMultipore PW-H	-	8 µm	N/A	2.0‒12.0	80 °C	1.000 ‒ 10.000.000 Da	N/A
TSKgel SuperOligoPW	-	3 µm	125 Å	2.0‒12.0	80 °C	100 ‒ 3.000 Da	N/A
TSKgel G3000PW_XL-CP	-	7 µm	200 Å	2.0‒12.0	80 °C	< 90.000 Da	N/A
TSKgel G5000PW_XL-CP		10 µm	1000 Å	2.0‒12.0	80 °C	< 1.000.000 Da	N/A
TSKgel G6000PW_XL-CP		13 µm	> 1000 Å	2.0‒12.0	80 °C	< 20.000.000 Da	N/A

TSKgel Alpha and SuperAW columns are based on highly crosslinked polymethacrylate. The columns are compatible with a wide range of solvents (from 100% water to 100% non-polar organic solvents) and were developed for the GPC analysis of polymers of intermediate polarity, soluble in water, buffers and many organic solvents.

Phase-Name	Modification	Particle size	Pore Size	pH-Range	T_max	Exclusion Limit (PEO)	Exclusion Limit (Dextrans)
TSKgel Alpha-2500	-	7 µm	25 Å	2.0‒12.0	80 °C	5.000 Da	10.000 Da
TSKgel Alpha-3000	-	7 µm	150 Å	2.0‒12.0	80 °C	90.000 Da	100.000 Da
TSKgel Alpha-4000	-	10 µm	450 Å	2.0‒12.0	80 °C	400.000 Da	1.000.000 Da
TSKgel Alpha-5000	-	10 µm	1000 Å	2.0‒12.0	80 °C	1.000.000 Da	7.000.000 Da
TSKgel Alpha-6000	-	13 µm	N/A	2.0‒12.0	80 °C	> 10.000.000 Da	> 10.000.000 Da
TSKgel Alpha-M	-	13 µm	N/A	2.0‒12.0	80 °C	> 10.000.000 Da	> 10.000.000 Da
TSKgel Super AW 2500	-	4 µm	25 Å	2.0‒12.0	80 °C	5.000 Da	8.000 Da
TSKgel Super AW 3000	-	4 µm	150 Å	2.0‒12.0	80 °C	90.000 Da	80.000 Da
TSKgel Super AW 4000	-	6 µm	450 Å	2.0‒12.0	80 °C	1.000.000 Da	600.000 Da
TSKgel Super AW 5000	-	7 µm	1000 Å	2.0‒12.0	80 °C	1.000.000 Da*	N/A
TSKgel Super AW 6000	-	9 µm	N/A	2.0‒12.0	80 °C	10.000.000 Da*	N/A
TSKgel Super AW M-H	-	9 µm	N/A	2.0‒12.0	80 °C	10.000.000 Da*	N/A

* Estimated Values

TSKgel H-Type columns are recommended for the analysis of organic soluble polymers and are packed with spherical particles composed of polystyrene crosslinked with divinylbenzene (PS-DVB). The packings are available in eight pore sizes and span four different columns chemistries. For polymer samples with a broad molecular range, packing of several pore sizes are provided in the mixed bed columns: TSKgel SuperHZM series, TSKgel SuperHM series, TSKgel GMHXL, TSKgel GMHHR, and selected high temperature versions provide linear calibration curves up to several million Daltons.

Phase-Name	Modification	Particle Size	Pore Size	pH-Range	T_max	Exclusion Limit (Polystyrene)
TSKgel G1000H_HR	-	5 µm	15 Å	1.0‒14.0	140 °C	< 1.000 Da
TSKgel G2000H_HR	-	5 µm	20 Å	1.0‒14.0	140 °C	< 10.000 Da
TSKgel G2500H_HR	-	5 µm	30 Å	1.0‒14.0	140 °C	20.000 Da
TSKgel G3000H_HR	-	5 µm	75 Å	1.0‒14.0	140 °C	< 60.000 Da
TSKgel G4000H_HR	-	5 µm	200 Å	1.0‒14.0	140 °C	< 400.000 Da
TSKgel G5000H_HR	-	5 µm	650 Å	1.0‒14.0	140 °C	< 4.000.000 Da
TSKgel G6000H_HR	-	5 µm	N/A	1.0‒14.0	140 °C	< 40.000.000 Da
TSKgel G7000H_HR	-	5 µm	N/A	1.0‒14.0	140 °C	400.000.000 Da
TSKgel GMH_HR-L mixed-bed	-	5 µm	N/A	1.0‒14.0	140 °C	< 4.000.000 Da
TSKgel GMH_HR-N mixed-bed	-	5 µm	N/A	1.0‒14.0	140 °C	< 4.000.000 Da
TSKgel GMH_HR-M mixed-bed	-	5 µm	N/A	1.0‒14.0	140 °C	< 4.000.000 Da
TSKgel GMH_HR-H mixed-bed	-	5 µm	N/A	1.0‒14.0	140 °C	< 400.000.000 Da
TSKgel G1000H_XL	-	5 µm	15 Å	1.0‒14.0	60 °C	1.000 Da
TSKgel G2000H_XL	-	5 µm	20 Å	1.0‒14.0	60 °C	10.000 Da
TSKgel G2500H_XL	-	5 µm	30 Å	1.0‒14.0	60 °C	20.000 Da
TSKgel G3000H_XL	-	5 µm	75 Å	1.0‒14.0	60 °C	60.000 Da
TSKgel G4000H_XL	-	5 µm	200 Å	1.0‒14.0	60 °C	400.000 Da
TSKgel G5000H_XL	-	9 µm	650 Å	1.0‒14.0	80 °C	4.000.000 Da
TSKgel G6000H_XL	-	9 µm	N/A	1.0‒14.0	80 °C	40.000.000 Da
TSKgel G7000H_XL	-	9 µm	N/A	1.0‒14.0	80 °C	400.000.000 Da
TSKgel GMH_XL mixed-bed	-	9 µm	N/A	1.0‒14.0	80 °C	400.000.000 Da
TSKgel GMH_XL-L mixed-bed	-	5 µm	N/A	1.0‒14.0	80 °C	4.000.000 Da
TSKgel Multipore H_XL-M	-	5 µm	N/A	1.0‒14.0	80 °C	2.000.000 Da
TSKgel SuperH1000	-	3 µm	15 Å	1.0‒14.0	140 °C	1.000 Da
TSKgel SuperH2000	-	3 µm	20 Å	1.0‒14.0	140 °C	10.000 Da
TSKgel SuperH2500	-	3 µm	30 Å	1.0‒14.0	140 °C	20.000 Da
TSKgel SuperH3000	-	3 µm	75 Å	1.0‒14.0	140 °C	60.000 Da
TSKgel SuperH4000	-	3 µm	200 Å	1.0‒14.0	140 °C	400.000 Da
TSKgel SuperH5000	-	3 µm	650 Å	1.0‒14.0	140 °C	4.000.000 Da
TSKgel SuperH6000	-	5 µm	N/A	1.0‒14.0	140 °C	40.000.000 Da
TSKgel SuperH7000	-	5 µm	N/A	1.0‒14.0	140 °C	400.000.000 Da
TSKgel SuperHM-L	-	3 µm	N/A	1.0‒14.0	140 °C	4.000.000 Da
TSKgel SuperHM-N	-	3 µm	N/A	1.0‒14.0	140 °C	400.000 Da
TSKgel SuperHM-M	-	3 µm	15 Å	1.0‒14.0	140 °C	2.000.000 Da
TSKgel SuperHM-H	-	3 µm	20 Å	1.0‒14.0	140 °C	400.000.000 Da
TSKgel SuperHZ1000	-	3 µm	30 Å	1.0‒14.0	60 °C	1.000 Da
TSKgel SuperHZ2000	-	3 µm	75 Å	1.0‒14.0	60 °C	10.000 Da
TSKgel SuperHZ2500	-	3 µm	200 Å	1.0‒14.0	60 °C	20.000 Da
TSKgel SuperHZ3000	-	3 µm	N/A	1.0‒14.0	60 °C	60.000 Da
TSKgel SuperHZ4000	-	3 µm	N/A	1.0‒14.0	80 °C	400.000 Da
TSKgel SuperHZM-N	-	3 µm	N/A	1.0‒14.0	80 °C	7.000.000 Da
TSKgel SuperHZM-M	-	3, 5 µm	140 Å	1.0‒14.0	80 °C	400.000.000 Da
TSKgel SuperHZM-H	-	10 µm	80 Å	1.0‒14.0	80 °C	400.000.000 Da
TSKgel SuperMultiporeHZ-M	-	4 µm	N/A	1.0‒14.0	N/A	2.000.000 Da
TSKgel SuperMultiporeHZ-N	-	3 µm	N/A	1.0‒14.0	N/A	120.000 Da
TSKgel SuperMultiporeHZ-H	-	6 µm	N/A	1.0‒14.0	N/A	40.000.000 Da
TSKgel GMH_HR-H (30) HT2	-	30 µm	N/A	1.0‒14.0	220 °C	400.000.000 Da
TSKgel GMH_HR-H (20) HT2	-	20 µm	N/A	1.0‒14.0	220 °C	400.000.000 Da
TSKgel GMH_HR-H (S) HT2	-	13 µm	N/A	1.0‒14.0	220 °C	400.000.000 Da
TSKgel G2000H_HR (20) HT2	-	20 µm	20 Å	1.0‒14.0	220 °C	10.000 Da
TSKgel GMH_HR-H (30) HT	-	13 µm	N/A	1.0‒14.0	170 °C	400.000.000 Da
TSKgel GMH_HR-H (20) HT	-	20 µm	N/A	1.0‒14.0	170 °C	400.000.000 Da
TSKgel GMH_HR-H (S) HT	-	13 µm	N/A	1.0‒14.0	170 °C	400.000.000 Da

Ion Exchange Chromatography

There are different silica-based TSKgel SW-Type columns for Ion Exchange Chromatography with various modifications (SAX, WAX, SCX, WCX).

Phase-Name	Modification	Particle Size	Pore Size	pH-Range	T_max	Exclusion Limit (PEG)	Capacity
TSKgel QAE-2SW	Trimethylamino	5 µm	125 Å	2.0‒7.5	N/A	10.000 Da	N/A
TSKgel DEAE-2SW	Diethylaminoethyl	5 µm	125 Å	2.0‒7.5	N/A	10.000 Da	N/A
TSKgel DEAE-3SW	Diethylaminoethyl	10 µm	250 Å	2.0‒7.5	N/A	30.000 Da	N/A
TSKgel SP-2SW	Sulfopropyl	5 µm	125 Å	2.0‒7.5	N/A	10.000 Da	N/A
TSKgel CM-2SW	Carboxymethyl	5 µm	125 Å	2.0‒7.5	N/A	10.000 Da	110 mg Hemoglobin/mL
TSKgel CM-3SW	Carboxymethyl	10 µm	250 Å	2.0‒7.5	N/A	30.000 Da	N/A

Polymethacrylate-based columns for SAX, WAX, SCX or WCX

Phase-Name	Modification	Particle Size	Pore Size	pH-Range	T_max	Exclusion Limit (PEG)	Capacity
TSKgel BioAssist Q	Polyamine	10, 13 µm	4000 Å	2.0‒12.0	N/A	5.000.000 Da	70 mg BSA/mL
TSKgel BioAssist S	Sulfopropyl	7, 13 µm	1300 Å	2.0‒12.0	N/A	~ 4.000.000 Da	70 mg γ-Globulin
TSKgel SuperQ-5PW	Trimethylamino	10, 13 µm	1000 Å	2.0‒12.0	N/A	1.000.000 Da	100 mg BSA/mL
TSKgel DEAE-5PW	Diethylaminoethyl	10, 13, 20 µm	1000 Å	2.0‒12.0	N/A	1.000.000 Da	30 mg BSA/mL
TSKgel SP-5PW	Sulfopropyl	10, 13, 20 µm	1000 Å	2.0‒12.0	N/A	1.000.000 Da	40 mg Hemoglobin/mL
TSKgel CM-5PW	Carboxymethyl	10, 13 µm	1000 Å	2.0‒12.0	N/A	1.000.000 Da	45 mg Hemoglobin/mL
TSKgel Q-STAT	Trimethylamino	7, 10 µm	Non-porous	3.0‒10.0	N/A	500 Da	20 mg BSA/mL
TSKgel DNA-STAT	Trimethylamino	5 µm	Non-porous	3.0‒10.0	N/A	500 Da	35 mg BSA/mL
TSKgel SP-STAT	Sulfopropyl	7, 10 µm	Non-porous	3.0‒10.0	N/A	500 Da	10 mg Lysozyme/mL
TSKgel CM-STAT	Carboxymethyl	7, 10 µm	Non-porous	3.0‒10.0	N/A	500 Da	15 mg Lysozyme/mL
TSKgel DEAE-NPR	Diethylaminoethyl	2.5 µm	Non-porous	2.0‒12.0	N/A	500 Da	5 mg BSA/mL
TSKgel DNA-NPR	Proprietary	2.5 µm	Non-porous	2.0‒12.0	N/A	500 Da	5 mg BSA/mL
TSKgel SP-NPR	Sulfopropyl	2.5 µm	Non-porous	2.0‒12.0	N/A	500 Da	5 mg Hemoglobin/mL

PS-DVB based columns for Ion Exchange Chromatography

Phase-Name	Modification	Particle Size	Pore Size	pH-Range	T_max	Exclusion Limit (PEG)	Capacity
TSKgel Sugar AXI	Trimethylamino	8 µm	60 Å	1.0‒14.0	N/A	N/A	N/A
TSKgel Sugar AXG	Trimethylamino	10 µm	60 Å	1.0‒14.0	N/A	N/A	N/A
TSKgel SAX	Trimethylamino	5 µm	60 Å	1.0‒14.0	N/A	N/A	N/A
TSKgel SCX	Sulfonic acid	5 µm	60 Å	1.0‒14.0	N/A	N/A	N/A

Hydrophilic Interaction Chromatography (HILIC)

TSKgel HILIC columns are well-suited for the analysis of polar and hydrophilic compounds. The unique polar phases can be operated over a broad range of mobile phase conditions. They are stable in 100% organic solvents and are able to separate many different types of polar molecules.

Phase-Name	Modification	Particle Size	Pore Size	End-capping	C-Content	USP	Surface	pH-Range	T_max
TSKgel Amide-80	Amide	2, 3, 5, 10 µm	100 Å	N/A	N/A	L68	450 m²/g	2.0‒7.5	80°C 50°C*
TSKgel NH₂-100	Amino	3 µm	100 Å	N/A	N/A	L8	450 m²/g	2.0‒7.5	50 °C

* For 3 µm Particle Size

Hydrophobic Interaction Chromatography (HIC)

TSKgel HIC phases are polymethacrylate-based with a choice of three ligands with varied hydrophobicities from low to high. TSKgel Phenyl-5PW is the most hydrophobic and Butyl-NPR the least hydrophobic among the three TSKgel HIC columns. The phases are physically and chemically stable in water soluble organic solvents (at < 50% methanol, ethanol, ACN, DMF, DMSO or < 30% chloroform).

Phase-Name	Modification	Particle Size	Pore Size	End-capping	C-Content	USP	Surface	pH-Bereich	T_max
TSKgel Phenyl-5PW	Phenyl	10 µm	1000 Å	N/A	N/A	N/A	N/A	2.0‒12.0	50 °C
TSKgel Etder-5PW	PEG	10 µm	1000 Å	N/A	N/A	N/A	N/A	2.0‒12.0	50 °C
TSKgel Butyl-NPR	Butyl	2.5 µm	Non-porous	N/A	N/A	N/A	N/A	2.0‒12.0	60 °C

Affinity Chromatography (AFC)

TSKgel Boronate-5PW carries m-aminophenyl boronate as ligand. Under alkaline conditions the boronate anionic structure of the TSKgel Boronate-5PW can bind with 1,2 cis-diol groups, such as those found in carbohydrates, carbohydrate-containing compounds and catecholamines. This interaction is enhanced in the presence of Mg²⁺ ions and inhibited by amine-containing buffers.

TSKgel Chelate utilizes the ability of iminodiacetic acid (IDA) to chelate ions such as Zn²⁺, Ni²⁺ and Cu²⁺. Peptides and Proteins containing histidine residues will normally adsorb to these chelated ions at neutral pH. The retained compounds are then eluted with buffer containing imidazole or glycine.

TSKgel Tresyl requires activation with a user-selected ligand containing amino, thiol, phenol, or imidazole groups.

The TSKgel Protein A-5PW column has been designed for the rapid separation and robust quantification of a variety of antibodies.

Phase-Name	Modification	Particle Size	Pore Size	End-capping	C-Content	USP	Surface	pH-Bereich	T_max
TSKgel Boronate-5PW	m-Aminophenyl-boronate	10 µm	1000 Å	N/A	N/A	N/A	N/A	2.0‒12.0	45 °C
TSKgel Chelate-5PW	Iminodiacetic acid	10 µm	1000 Å	N/A	N/A	N/A	N/A	2.0‒9.0	N/A
TSKgel Tresyl-5PW	Tresyl	10 µm	1000 Å	N/A	N/A	N/A	N/A	2.0‒12.0	N/A
TSKgel Protein A-5PW	Recombinant Protein A, Hexamer of C Domain	20 µm	1000 Å	N/A	N/A	N/A	N/A	2.0‒12.0	30°C

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