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TSKgel FcR-IIIA-NPR
For the fast assessment for biologic activity of mAbs
Tosoh Bioscience GmbH introducing the new affinity column - TSKgel FcR-IIIA-NPR - based on a recombinant FcγIIIa receptor ligand. The column is designed for fast assessment for biologic activity of mAbs.
The FcγIIIa receptor plays a key role in antibody-dependent cell mediated cytotoxicity (ADCC) by interacting with the N-glycans of the Fc domain of immunoglobulins. Hence, affinity chromatography using immobilized Fc receptor ligands can deliver valuable information about ADCC activity of therapeutic antibodies. As the interaction/affinity is influenced by N-glycans the separation profile also reflect the antibodies glycoform pattern.
This fast and efficient method can be used in many phases of development and production such as cell line screening in early R&D, biosimilar/originator comparison, upstream development and optimization, monitoring of glycoengineering, or lot-to-lot comparison in QC.
Technical Data
Highlights of the product:
- Easy and reproducible HPLC analysis based on FcγRIIIa affinity of mAbs
- Unique glycoprotein elution profile of IgG allows assessment of ADCC activity
- Applicable to purified samples and cell culture supernatant alike
- Fast cell line screening, upstream development, lot-to-lot comparison
| Description | Matrix | Ligand | Particle size | pH-Range | T-Range | Housing | Dimension |
| TSKgel® FcR-IIIA-NPR | Hydrophilic non-porous Polymer | Modified recombinant human FcγRIIIA | 5 µm | 4.0−8.0 | 15−25°C | PEEK | 75x4.6mm |
Downloads
Brochures
Tosoh TSKgel FcR-IIIA-NPR Fast Evaluation of ADCC Activity Applications
Technical Support
Tosoh TSKgel FcR-IIIA-NPR Product Manual Technical-Support
Tosoh TSKgel FcR-IIIA-NPR Robustness and Dynamic Range Technical-Support
Applications
Tosoh TSKgel FcR-IIIA-NPR Characterization by Top Down MS Applications
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