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ProPac
The ProPacâ„¢ product range, manufactured by Thermo Scientificâ„¢, consists of various HPLC columns for ion-exchange, hydrophobic interaction and affinity chromatography. They are suitable for the analysis of diverse biological samples containing proteins, peptides and other biomolecules.
The ProPac™ SAX-10, WAX-10, SCX-10, SCX-20 and WCX-10 protein columns are ion exchange columns designed specifically to provide high-resolution and high efficiency separations of proteins and glycoproteins (pI = 3‒10, MW>10,000 DA). The stationary phase is composed of 10 µm, non-porous ethylvinylbenzene-divinylbenzene copolymer beads which are surrounded by a highly hydrophilic, neutral polymer to reduce non-specific interactions between the surface and the biopolymer. The surface is grafted with the respective functional groups.
Technical Data
Columns for Ionexchange-Chromatography:
| Phase-Name | Modifizierung | Particle Size | Pore Size | pH-Range | Tmax | "Break-through"-Capacity |
| ProPac™ SAX-10 | Quaternary Ammonium | 10 µm | Non-porous | 2.0‒12.0 | 60 °C | 15 mg BSA/mL Column Volume |
| ProPac™ WAX-10 | Tertiary Amine | 10 µm | Non-porous | 2.0‒12.0 | 60 °C | 3 mg BSA/mL Column Volume |
| ProPac™ SCX-10 | Sulfonic Acid | 10 µm | Non-porous | 2.0‒12.0 | 60 °C | 3 mg Lysozyme/mL Column Volume |
| ProPac™ SCX-20 | Sulfonic Acid | 10 µm | Non-porous | 2.0‒12.0 | 60 °C | N/A |
| ProPac™ WCX-10 | Carboxylic Acid | 10 µm | Non-porous | 2.0‒12.0 | 60 °C | 6 mg Lysozyme/mL Column Volume |
| ProPac™ PA1 | Quaternary Ammonium | 10 µm | Non-porous | 2.0‒12.0 | N/A | N/A |
Columns for Hydrophobic Interaction Chromatography:
| Phase-Name | Modification | Particle Size | Pore Size | End-capping | Surface | pH-Range | Tmax | "Break-through"-Capacity |
| ProPac™ HIC-10 | Amide/Ethyl | 5 µm | 300 Å | - | 100 m2/g | 2.5‒7.5 | 40 °C | 340 mg Lysozyme |
Columns for Affinity Chromatography:
| Phase-Name | Fuctional Ligand | Particle Size | Pore Size | pH-Range | Tmax | Protein Binding Capacity |
| ProPac™ IMAC-10 | Iminodiacetate (IDA) grafts | 10 µm | Non-porous | 2.0‒12.0 | 60 °C | 1‒5 mg Lysozyme/g resin |
Downloads
Brochures
Thermo ProPac Elite WCX HPLC Life Science Brochure
Thermo ProPac HIC 10 HPLC Life Science Brochure
Thermo ProPac IMAC 10 HPLC Life Science Brochure
Technical Support
ProPac PA1 Columns Product Manual
Thermo ProPac SCX-20 Product Manual
Thermo ProPac WAX-10, ProPac SAX-10 Product Manual
Thermo ProPac WCX-10, ProPac SCX-10 Product Manual
Applications
Thermo Propac Elite WCX pH Gradient Analysis of IgG1 HPLC Life Science Applications
Thermo Propac Elite WCX Therapeutic Protein Analysis HPLC Life Science Applications
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