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Acclaim
Acclaim™ HPLC columns originally belonged to Dionex Corporation, which has been part of the Thermo Fisher Scientific Group since 2011. Acclaim™ HPLC and UHPLC columns are based on high purity spherical silica gel, which are available with many different modifications and selectivities. Reliable and reproducible (U)HPLC columns are obtained through a uniform production process with strict quality specifications. Acclaim HPLC columns are also available with application-specific modifications.
Thermo Scientific Acclaim™SEC-300 and SEC-1000 are high-efficiency size exclusion chromatography (SEC) columns specifically designed for separation of water-soluble polymers and oligomers, e.g. polyethylene glycols, polyacrylic acids, dextrans and proteins, respectively. They are packed with spherical, hydrophilic polymethacrylate particles and provide high-resolution and good linearity of the calibration curve for accurate and reliable analysis. Acclaim SEC columns offer two pore sizes, 300 Å and 1000 Å, to cover a broad molecular weight range.
Technical Data
Acclaim
| Phase name | Modification | Particle size | Pore size | End-capping | Carbon content | USP | BET surface area | pH-Range | Tmax |
| C30 | C30 | 3,5 µm | 200 Å | Proprietary | 13% | L62 | 200 m2/g | 2.0-8.0 | 60°C |
| 120 C18 | C18 | 2.2, 3,5 µm | 120 Å | + | 18% | L1 | 300 m2/g | 2.0-8.0 | 60°C |
| 120 C8 | C8 | 2.2, 3,5 µm | 120 Å | + | 11% | L7 | 300 m2/g | 2-8 | 60°C |
| Phenyl-1 | Phenyl | 3,5 µm | 120 Å | + | 13% | L11 | 300 m2/g | 2-8 | 60°C |
| Polar Advantage | Sulfonamide embedded RP | 2.2, 3,5 µm | 120 Å | + | 17% | L60 | 300 m2/g | 2-8 | 60°C |
| Polar Advantage II | Amide embedded RP | 2.2, 3,5 µm | 120 Å | + | 17% | L60 | 300 m2/g | 1.5-10* | 60°C |
| HILIC-10 | Proprietary Polar Group | 3,5 µm | 120 Å | + | 8% | N/A | 300 m2/g | 2-8 | 70°C |
| Mixed-Mode HILIC-1 | Alkyl Diol | 3,5 µm | 120 Å | Proprietary | N/A | N/A | 300 m2/g | 2.5-7.5 | 50°C |
| Mixed-Mode WAX-1 | Hydrophobic alkyl chain witd an ionizable terminus | 3,5 µm | 120 Å | Proprietary | N/A | L78 | 300 m2/g | 2.5-7.5 | 50°C |
| Mixed-Mode WCX-1 | Hydrophobic alkyl chain witd a carboxyl terminus | 3,5 µm | 120 Å | Proprietary | N/A | L85 | 300 m2/g | 2.5-7.5 | 50°C |
Acclaim SEC
| Phase name | Modification | Particle size | Pore size | USP | pH-Range | Tmax | Exclusion Limit (PEO) |
| Acclaim SEC-300 | N/A | 5 µm | 300 Å | N/A | 2.0‒12.0 | 60°C | 50.000 ‒ 150.000 Da |
| Acclaim SEC-1000 | N/A | 7 µm | 1000 Å | N/A | 2.0‒12.0 | 60 °C | 3.000.000 ‒ 7.500.000 Da |
Application specific Acclaim (U)HPLC Columns:
| Column | Application |
| Acclaim AmG C18 | Aminoglycoside antibiotics separation |
| Acclaim Trinity P1 and P2 | API & counterion analysis |
| Acclaim Organic acid | Fast organic acid analysis |
| Acclaim Surfactant and Surfactant Plus | Separation of surfactants |
| Acclaim Explosives | Separation of explosive residues |
| Acclaim Trinity Q1 | Diquat and paraquat analysis |
| Acclaim Carbamate | Separation of carbamate pesticides |
| Acclaim Carbonyl C18 | Separation of DNPH derivates of aldehydes and ketones |
Downloads
Brochures
Thermo Acclaim Mixed Mode HPLC Columns HPLC Brochure
Thermo Acclaim SEC Brochure
Technical Support
Thermo Acclaim Column Selection Guide
Acclaim Organic Acid (OA)
Thermo Acclaim SEC Product Manual
Thermo Acclaim Trinity P2 Product Manual
Applications
Thermo Acclaim Mixed Mode WAX 1 Analysis of Additives in Soft Drinks HPLC Applications
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