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Acclaim
Acclaim™ HPLC columns originally belonged to Dionex Corporation, which has been part of the Thermo Fisher Scientific Group since 2011. Acclaim™ HPLC and UHPLC columns are based on high purity spherical silica gel, which are available with many different modifications and selectivities. Reliable and reproducible (U)HPLC columns are obtained through a uniform production process with strict quality specifications. Acclaim HPLC columns are also available with application-specific modifications.
Thermo Scientific Acclaim™SEC-300 and SEC-1000 are high-efficiency size exclusion chromatography (SEC) columns specifically designed for separation of water-soluble polymers and oligomers, e.g. polyethylene glycols, polyacrylic acids, dextrans and proteins, respectively. They are packed with spherical, hydrophilic polymethacrylate particles and provide high-resolution and good linearity of the calibration curve for accurate and reliable analysis. Acclaim SEC columns offer two pore sizes, 300 Å and 1000 Å, to cover a broad molecular weight range.
Technical Data
Acclaim
| Phase name | Modification | Particle size | Pore size | End-capping | Carbon content | USP | BET surface area | pH-Range | Tmax |
| C30 | C30 | 3,5 µm | 200 Å | Proprietary | 13% | L62 | 200 m2/g | 2.0-8.0 | 60°C |
| 120 C18 | C18 | 2.2, 3,5 µm | 120 Å | + | 18% | L1 | 300 m2/g | 2.0-8.0 | 60°C |
| 120 C8 | C8 | 2.2, 3,5 µm | 120 Å | + | 11% | L7 | 300 m2/g | 2-8 | 60°C |
| Phenyl-1 | Phenyl | 3,5 µm | 120 Å | + | 13% | L11 | 300 m2/g | 2-8 | 60°C |
| Polar Advantage | Sulfonamide embedded RP | 2.2, 3,5 µm | 120 Å | + | 17% | L60 | 300 m2/g | 2-8 | 60°C |
| Polar Advantage II | Amide embedded RP | 2.2, 3,5 µm | 120 Å | + | 17% | L60 | 300 m2/g | 1.5-10* | 60°C |
| HILIC-10 | Proprietary Polar Group | 3,5 µm | 120 Å | + | 8% | N/A | 300 m2/g | 2-8 | 70°C |
| Mixed-Mode HILIC-1 | Alkyl Diol | 3,5 µm | 120 Å | Proprietary | N/A | N/A | 300 m2/g | 2.5-7.5 | 50°C |
| Mixed-Mode WAX-1 | Hydrophobic alkyl chain witd an ionizable terminus | 3,5 µm | 120 Å | Proprietary | N/A | L78 | 300 m2/g | 2.5-7.5 | 50°C |
| Mixed-Mode WCX-1 | Hydrophobic alkyl chain witd a carboxyl terminus | 3,5 µm | 120 Å | Proprietary | N/A | L85 | 300 m2/g | 2.5-7.5 | 50°C |
Acclaim SEC
| Phase name | Modification | Particle size | Pore size | USP | pH-Range | Tmax | Exclusion Limit (PEO) |
| Acclaim SEC-300 | N/A | 5 µm | 300 Å | N/A | 2.0‒12.0 | 60°C | 50.000 ‒ 150.000 Da |
| Acclaim SEC-1000 | N/A | 7 µm | 1000 Å | N/A | 2.0‒12.0 | 60 °C | 3.000.000 ‒ 7.500.000 Da |
Application specific Acclaim (U)HPLC Columns:
| Column | Application |
| Acclaim AmG C18 | Aminoglycoside antibiotics separation |
| Acclaim Trinity P1 and P2 | API & counterion analysis |
| Acclaim Organic acid | Fast organic acid analysis |
| Acclaim Surfactant and Surfactant Plus | Separation of surfactants |
| Acclaim Explosives | Separation of explosive residues |
| Acclaim Trinity Q1 | Diquat and paraquat analysis |
| Acclaim Carbamate | Separation of carbamate pesticides |
| Acclaim Carbonyl C18 | Separation of DNPH derivates of aldehydes and ketones |
Downloads
Brochures
Thermo Acclaim Mixed Mode HPLC Columns HPLC Brochure
Thermo Acclaim SEC Brochure
Applications
Thermo Acclaim Mixed Mode WAX 1 Analysis of Additives in Soft Drinks HPLC Applications
Technical Support
Thermo Acclaim Column Selection Guide
Acclaim Organic Acid (OA)
Thermo Acclaim SEC Product Manual
Thermo Acclaim Trinity P2 Product Manual
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