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SMT SAM
SMT SAM columns from SMT are based on the self-assembled monolayer (SAM) technique. This bonding technique allows ordered monolayers of functional groups. This is also called "horizontal polymerisation", due to Si-O-Si bonds running parallel to the silica surface. As a result, a higher ligand density can be achieved compared to conventional "vertical polymerization".
This SAM technology is available for reversed phase, ion exchange, affinity, chiral chromatography as well as for UHPLC.
Technical Data
Reversal phase
| Phase | Modification | Particle size | Pore size | Surface area | Carbon content | USP Code |
| SAM-C18 OD | C18 | 3, 5, 10 μm 5, 10 μm 5 μm | 100 Å 300 Å 60 Å | 340 m²/g 90 m²/g N/A | 24 %C 8 %C N/A | L1 |
| SAM-C18 ODL | C18 | 3, 5, 10 μm 5 μm | 100 Å 300 Å | 340 m²/g 90 m²/g | 12 %C 4 %C | L1 |
| SMT Elite-C18 | C18 | 5, 10 μm | 100 Å | 340 m²/g | 16 %C | L1 |
| SAM-C8 O | C8 | 3, 5, 10 μm 5, 10 μm 5 μm | 100 Å 300 Å 60 Å | 340 m²/g 90 m²/g N/A | 12 %C 5 %C N/A | L7 |
| SAM-C8 OL | C8 | 3, 5, 10 μm 5, 10 μm | 100 Å 300 Å | 340 m²/g 90 m²/g | 6 %C 2 %C | L7 |
| SMT Elite-C8 | C8 | 5, 10 μm | 100 Å | 340 m²/g | 8 %C | L7 |
| SMT MEB1 | C1 | 5 μm 5 μm | 100 Å 300 Å | 340 m²/g 90 m²/g | 1 %C 0.6 %C | L13 |
| SMT MEB2 | C2 | 5 μm 5 μm | 100 Å 300 Å | 340 m²/g 90 m²/g | 2 %C 1 %C | L30 |
| SMT MEB4 | C4 | 5 μm 5 μm | 100 Å 300 Å | 340 m²/g 90 m²/g | 4 %C 2 %C | L26 |
| SMT Phen1 | Ph (one per ligand) | 5, 10 μm 5, 10 μm | 100 Å 300 Å | 340 m²/g 90 m²/g | 7.1 %C 3.1 %C | L11 |
| SMT Phen2 | Ph (two per ligand) | 5, 10 μm 5, 10 μm | 100 Å 300 Å | 340 m²/g 90 m²/g | 8.4 %C 3.6 %C | L11 |
| SMT PhenH | Ph-hexyl | 3, 5, 10 μm 5, 10 μm | 100 Å 300 Å | 340 m²/g 90 m²/g | 7.1 %C 3.1 %C | N/A |
| SMT SAM C3 | C3 | 5 µm 5 µm | 100 Å 300 Å | 340 m²/g 90m²/g | 6.5 %C 3.6 %C | N/A |
| SMT SAM C5 [Pen] | C5 | 5 µm 5 µm | 100 Å 300 Å | 340 m²/g 90 m²/g | 6.5 %C 3.6 %C | N/A |
| SMT SAM C6 [Hex] | C6 | 5 µm 5 µm | 100 Å 300 Å | 340 m²/g 90 m²/g | 6.5 %C 3.6 %C | N/A |
| SMT SAM C12 [DD] | C12 | 5 µm 5µm | 100 Å 300 Å | 340 m²/g 90 m²/g | 11 %C 5 %C | N/A |
Ion exchange
| Phase | Modification | Particle size | Pore size | Surface area | USP Code |
| SMT SAX | quaternary amine | 5, 10 μm 5, 10 μm | 100 Å 300 Å | 340 m²/g 90 m²/g | L14 |
| SMT WAX | polyethyleneimine | 5, 10 μm 5, 10 μm | 100 Å 300 Å | 340 m²/g 90 m²/g | N/A |
| SMT DEAE | di-ethyl-amino-ethyl | 5, 10 μm 5, 10 μm | 100 Å 300 Å | 340 m²/g 90 m²/g | N/A |
| SMT SCX | sulfonic acid | 5, 10 μm 5, 10 μm | 100 Å 300 Å | 340 m²/g 90 m²/g | L9, L52 |
| SMT WCX | carboxylic acid | 5, 10 μm 5, 10 μm | 100 Å 300 Å | 340 m²/g 90 m²/g | N/A |
Affinity chromatography
| Phase | Modification | Particle size | Pore size | Surface area | Carbon content | USP Code |
| SMT CIB-ProteinA | ProteinA | 5, 10 μm | 100 Å | N/A | N/A | N/A |
| SMT CIB-IgG | IgG | 5, 10 µm | 100 Å | N/A | N/A | N/A |
Chiral phases
| Phase | Modification | Particle size | Pore size | Surface area | Carbon content | USP Code |
| SMT ChiralSep1 | N/A | 5, 10 μm 5, 10 µm | 100 Å 300 Å | N/A | N/A | N/A |
| SMT ChiralSep2 | N/A | 5, 10 µm 5, 10 µm | 100 Å 300 Å | N/A | N/A | N/A |
UHPLC phases
| Phase | Modification | Particle size | Pore size | Surface area | Carbon content | USP Code |
| SMT SAM-C18 | C18 | 1.5, 1.8 μm | 100 Å | N/A | N/A | N/A |
| SMT SAM-C8 | C8 | 1.5, 1.8 µm | 100 Å | N/A | N/A | N/A |
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