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OligoMg
OligoMg columns from Sielc have been specially developed for the analysis of oligonucleotides. These columns are also based on the Bridge Ion Separation Technique (BIST) and have a negatively charged surface loaded with magnesium 2+ (Mg2+). In the presence of an organic modifier, these cations on the stationary phase enable the interaction between the negatively charged oligonucleotide and the stationary phase. The magnesium cations act as a bridge. This has the advantage that oligonucleotides can be effectively separated with a mobile phase consisting only of water and methanol.
Applications
Influence of the water content on the retention of oligonucleotides with the OligoMg from Sielc
Based on BIST technology, the OligoMg column has dual positively charged ions loaded onto the surface. This means that it is no longer necessary to add these divalent ions to the mobile phase. This means that oligonucleotides can be separated without the addition of a buffer
Peak identities
Oligo 5T (TTT TT)
Test conditions
Column: OligoMg 100x4.6mm, 3µm
Mobile phase: Acetonitrile/water
Flow rate: 1.0 mL/min
Detection: UV 260 nm
Temperature: 30 °C
Separation of different 6mer oligonucleotides with the OligoMG of Sielc
The geometry of different oligonucleotides influences the bridge binding between analytes and the stationary phase and thus the retention. In addition to the geometry, the strength and composition of the nucleobases also play a decisive role. This allows different oligonucleotides with the same chain length to be separated from each other.
Peak identities
1. oligo 3A3T (AAA TTT) 2. oligo 6A (AAA AAA) 3. oligo 3A3C (AAA CCC) 4. 3A3G (AAA GGG)
Test conditions
Column: OligoMg 150x4.6mm, 3µm
Mobile phase: Gradient acetonitrile - 65-45% in 10 min
Flow rate: 1.0 mL/min
Detection: UV 260 nm
Temperature: 30 °C
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Sielc BIST OligoMg Oligonucleotides
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