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Asahipak
Asahipakâ„¢ is a brand name for various HPLC columns made by Shodexâ„¢ (Showa Denkoâ„¢). The common feature of all Asahipakâ„¢ columns is the base material: polyvinyl alcohol. Asahipakâ„¢ columns include phases for reversed-phase chromatography (ODP-50, ODP-40, C8P-50, C4P-50), hydrophilic interaction chromatography (NH2P), size exclusion chromatography (GF-HQ), ion exchange chromatography ( ES-502N, ES-502C) and one multimode phase (GS-HQ).
Technical Data
Reversed Phase
| Phase-Name | Modification | Particle Size | Pore Size | End-capping | C-Content | USP | Surface | pH-Range | Tmax |
| Asahipak™ ODP-40 | C18 | 4 µm | 250 Å | - | 17% | L67 | N/A | 2.0-13.0 | 60°C |
| Asahipak™ ODP-50 | C18 | 5 µm | 250 Å | - | 17% | L67 | N/A | 2.0-13.0 | 60°C |
| Asahipak™ C8P-50 | C8 | 5 µm | 250 Å | - | 10% | N/A | N/A | 2.0-13.0 | 60°C |
| Asahipak™ C4P-50 | C4 | 5 µm | 250 Å | - | 6% | N/A | N/A | 2.0-13.0 | 60°C |
Hydrophilic interaction chromatography (HILIC)
Why should you choose Asahipak NH2P-50 for sugar separation?
- Polymer-based packing material
- Excellent chemical stability
- Extended lifetime compare to silica-based
- Minimum deterioration over extended time period
- Wider pH range from 2 to 13 compared to silica-based
- Washable with alkaline solution
- Suitable for evaporative light scattering detector, corona charged aerosol detector and LC/MS detectors
| Phase-Name | Modification | Particle Size | Pore Size | End-capping | C-Content | USP | Surface | pH-Range | Tmax |
| Asahipak™ NH2P-40 | Amino (Polyamine) | 4 µm | 100 Å | - | N/A | N/A | N/A | 2.0-13.0 | 50°C |
| Asahipak™ NH2P-50 Advantage II | Amino (Polyamine) | 5 µm | 100 Å | - | N/A | N/A | N/A | 2.0-13.0 | 50°C |
Size Exclusion Chromatography (SEC)
| Phase-Name | Particle size | Pore size | Molecular range | Exclusion limit | pH range | Tmax |
| Asahipak™ GF-210 HQ | 5 µm | 180 Å | 300 - 4.000 Da | 9.000 Da | 2.0‒9.0 | 60°C |
| Asahipak™ GF-310 HQ | 5 µm | 400 Å | 300 - 30.000 Da | 40.000 Da | 2.0-9.0 | 60°C |
| Asahipak™ GF-510 HQ | 5 µm | 2.000 Å | 5.000 - 200.000 Da | 300.000 Da | 2.0-9.0 | 60°C |
| Asahipak™ GF-7M HQ | 9 µm | 10.000 Å | 300 - 10.000.000 Da | 10.000.000 | 2.0-9.0 | 60°C |
Ion Exchange Chromatography (IEX)
| Phase-Name | Modification | Particle Size | Pore Size | End-capping | C-Content | USP | Surface | pH-Range | Tmax |
| Asahipak™ ES-502N[2] | Dietdylaminoethyl | 9 µm | 2000 Å | - | N/A | N/A | N/A | 2.0‒9.0 | 50°C |
| Asahipak™ ES-502C[2] | Carboxymethyl | 9 µm | 2000 Å | - | N/A | N/A | N/A | 2.0‒9.0 | 50°C |
Mixed-Mode chromatography
Asahipakâ„¢ GS-HQ is an HPLC columns with SEC as the main separation mode. With the choice of eluent, the column provides multimode features of reversed phase, hydrophilic interaction (HILIC) and ion exchange modes to SEC. The columns are suitable for the separation of peptides or nucleic acids with similar molecular weights and for desalting samples or substituting buffer in protein analysis. The base material is Polyvinyl alcohol.
| Phase-Name | Modification | Particle Size | Pore Size | USP | pH-Range | Tmax | MW-Range (Pullulan) | Exclusion Limit |
| Asahipak™ GS-220 HQ | N/A | 6 µm | 150 Å | N/A | 2.0‒9.0 | 60 °C | 300 ‒ 3.000 Da | 7.000 Da |
| Asahipak™ GS-320 HQ | N/A | 6 µm | 400 Å | N/A | 2.0‒12.0 | 60 °C | 300 ‒ 20.000 Da | 40.000 Da |
| Asahipak™ GS-520 HQ | N/A | 7 µm | 2000 Å | N/A | 2.0‒12.0 | 60 °C | 5.000 ‒ 200.000 Da | 300.000 Da |
| Asahipak™ GS-620 HQ | N/A | 7 µm | 7000 Å | N/A | 2.0‒12.0 | 60 °C | 10.000 ‒ 800.000 Da | 1.000.000 Da |
Downloads
Brochures
Shodex HPLC Columns Catalog 2023-2024
Applications
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Shodex Asahipak C8P-50 4D High Molecular Weight Peptides Applications
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Shodex Asahipak NH2P-40 2D Analysis of Various Steviol Glycosides Applications
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Shodex Asahipak NH2P-40 2D Analysis of Various Sweeteners Applications
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Shodex Asahipak NH2P HILIC Sugars Applications
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Shodex Asahipak ODP-50 6D Vitamin B6 Applications
Technical Support
Shodex Technical Notebook No. 2 Asahipak NH2P-50 Technical-Support
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