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SRT-C, Zenix-C, Unix-C
SRT-C, Zenix-C and Unix-C columns are silica based columns. Like the SRT, Zenix and Unix phases, the SRT-C, Zenix-C and Unix-C particles are coated with an uniform, hydrophilic and neutral film. However, this monolayer is a lay-down layer instead of a stand-up monolayer.
This is ideal for the separation of all biological samples types including challenging hydrophobic samples like antibody drug conjugates (ADCs), hydrophobic mAb and its fragments, bispecific mAbs (BsMAbs), PEGylated proteins, glycoproteins, fusion proteins, membrane proteins and oligonucleotides.
Technical Data
SRT-C
| Phase | Particle size | Pore size | Molecular range |
| SRT-C SEC-100 | 5, 10 μm | 100 Å | 100 - 100,000 (100 - 30,000 for 10 μm particle) Da |
| SRT-C SEC-150 | 5 μm | 150 Å | 500 - 150,000 Da |
| SRT-C SEC-300 | 5, 10 μm | 300 Å | 5,000 - 1,250,000 Da |
| SRT-C SEC-500 | 5, 10 μm | 500 Å | 15,000 - 5,000,000 Da |
| SRT-C SEC-1000 | 5, 10 μm | 1000 Å | 50,000 - 7,500,000 Da |
| SRT-C SEC-2000 | 5 μm | 2000 Å | >10,000,000 Da |
Zenix-C
| Phase | Particle size | Pore size | Molecular range |
| Zenix-C SEC-80 | 3 μm | 80 Å | 100 - 50,000 Da |
| Zenix-C SEC-100 | 3 μm | 100 Å | 100 - 100,000 Da |
| Zenix-C SEC-150 | 3 μm | 150 Å | 500 - 150,000 Da |
| Zenix-C SEC-300 | 3 μm | 300 Å | 5,000 - 1,250,000 Da |
Unix-C
| Phase | Particle size | Pore size | Molecular range |
| Unix-C SEC-300 | 1.8 μm | 300 Å | 5,000 - 1,250,000 Da |
Applications
Analysis of MAb on SRT®-C 300
Determination of MAb with SRT-C300.
Test Conditions:
Column: SRT®-C 300 (5 μm, 300 Å, 7.8x300 mm)
Mobile Phase: 150 mM sodium phosphate buffer, pH 7
Temperature: ambient (~23° C)
Flow rate: 1.0 mL/min
Detection: UV 214 nm
Concentration: 5 mg/mL
Injection volume: 2.5 μL
Sample: MAb
MAb321 Separation on Unix-C SEC-300
Seperation of MAb321 on Unix-C SEC-300.
Test Conditions:
Column: Unix-C SEC-300 (1.8 μm, 300 Å, 4.6 x 300 mm)
Mobile Phase: 150 mM phosphate buffer
Flow rate: 0.3 mL/min
System: UHPLC
Detection: UV 280 nm
Column temperature: ambient
Injection volume: 2μL
Sample: 5mg/ml MAb321
Downloads
Brochures
Sepax SRT 1010c SEC Life-Science Brochure
Sepax SRT SEC Life-Science Brochure
Unix-C SEC-300 UHPLC
Zenix-80
Applications
Sepax SRT-C 300 Analysis of MAb SEC
Sepax Unix-C SEC-300 MAb Separation
Sepax Zenix-C SEC-300 Rituximab Analysis
Sepax Zenix SEC 300 Aggregated Antibody Sample Analysis
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