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Monomix
Monomix columns from Sepax include various modifications for special applications. All phases are based on polymers.
With the Monomix Aminodiol and the dT20 Affinity, two modifications are available for affinity chromatography.
The Monomix Core Phase is a core-shell material based on polymers. Various surface modifications and a special pore structure make different interactions possible.
Monomix H2P SAX is a special phase for the determination of surfactants.
Monomix IEX includes various modifications for anion and cation exchange chromatography.
Monomix Mab60-SP is a material with a pore size of 1000Å and cation exchange functionalisation. Therefore this material is suitable for the analysis of antibodies, vaccines, insulin, proteins, nucleic acids and heparin.
Monomix MC-HIC are phases for hydrophobic intercation chromatography. Various modifications are available for different selectivities.
MonomixSEC are materials for size exclusion chromatography. The phases are compatible with many common organic solvents and aqueous buffers.
Technical Data
Monomix Affinity Chromatography
| Phase | Modification | matrix | Particle size | Pore size | pH range | Temperature range |
| Monomix MC30-Aminodiol | Aminodiol | Hydrophilic polymethacrylate | 30 µm | 500, 1000 Å | 2.0 - 12.0 | 4 - 40 °C |
| Monomix dT(20) | Oligo dT20-mer | Hydrophilic polymethacrylate | 30 µm | 1000 Å | 2.0 - 12.0 | 4 - 65°C |
Monomix Core
| Phase | Modification Core | Shell modification | Pore size | Exclusion limit | Ion exchange capacity of the core | pH range | Tmax |
| Monomix Core | Amines | Hydrophilic groups | 500 Å | 400,000 Da | 80 - 300 µmol Cl-/mL Medium | 2.0 - 13.0 | ≤40 °C |
Monomix H2P SAX
| Phase | Modification | Particle size | Ion exchange capacity | pH range | Tmax |
| Monomix H2P SAX | Mixed-Mode | 40 µm | 0.5 meq/g | 1.0 - 13.0 | 80 °C |
Monomix IEX
| Phase | Modification | Particle size | Pore size | Dynamic binding capacity | pH range | Temperature range |
| Monomix HC-Q | quaternary ammonium | 30, 60 µm | 500, 1000Å | >100 mg BSA | 2.0 - 12.0 | ≤40 °C |
| Monomix MC-Q | quaternary ammonium | 30, 60 µm | 500, 1000Å | >51 mg BSA | 2.0 - 12.0 | ≤40 °C |
| Monomix HC-DEAE | Diethylaminoethyl | 30, 60 µm | 500, 1000Å | >90 mg BSA | 2.0 - 12.0 | ≤40 °C |
| Monomix MC-DEAE | Diethylaminoethyl | 30, 60 µm | 500, 1000Å | >47 mg BSA | 2.0 - 12.0 | ≤40 °C |
| Monomix HC-SP | Sulfonic acid | 30, 60 µm | 500, 1000Å | >105 mg Lysozymes | 2.0 - 12.0 | ≤40 °C |
| Monomix MC-SP | Sulfonic acid | 30, 60 µm | 500, 1000Å | >52 mg Lysozymes | 2.0 - 12.0 | ≤40 °C |
Monomix Mab60-SP
| Phase | Modification | Particle size | Pore size | Dynamic binding capacity | pH range |
| Monomix Mab60-SP | Sulfonic acid | 60 µm | 1000 Å | ≥60 mg lysozymes / mL medium | 1.0 - 14.0 |
Monomix MC-HIC
| Phase | Modification | Particle size | Pore size | Dynamic binding capacity | pH range | Tmax |
| Monomix MC-HIC Ether | Ether | 30 µm | 1000 Å | >20 mg Lysozymes/mL medium | 2.0 - 13.0 | 40 °C |
| Monomix MC-HIC Butyl | C4 | 30 µm | 1000 Å | >40 mg Lysozymes/mL medium | 2.0 - 13.0 | 40 °C |
| Monomix MC-HIC Phenyl | Phenyl | 30 µm | 1000 Å | >45 mg Lysozymes/mL medium | 2.0 - 13.0 | 40 °C |
Monomix SEC
| Phase | Particle size | Pore size | Molecular area PEO or PEG | Molecular area Dextran | Molecular range globular protein | pH range | Tmax |
| Monomix MC10 SEC | 10 µm | 500 Å | N/A | N/A | N/A | 2.0 - 12.0 | 80 °C |
| Monomix MC15 SEC | 15 µm | 500 Å | N/A | N/A | N/A | 2.0 - 12.0 | 80 °C |
| Monomix MC30 SEC | 30 µm | 500 Å | N/A | N/A | N/A | 2.0 - 12.0 | 80 °C |
| Monomix MC60 SEC | 60 µm | 500 Å | N/A | N/A | N/A | 2.0 - 12.0 | 80 °C |
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