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Porvair Plates
Porvair plates are well plates for various applications. There is a large selection of different geometries, volumes, materials and plate dimensions. The portfolio ranges from reservoirs, assay plates and cell culture plates to storage plates and filtration plates.
Reservoirs are containers from Porvair Sciences and are designed for use with all robotic liquid handling systems. Reservoir trays are available for different working configurations, e.g. for 8 or 12 channel pipettes up to 96 or 384 pipette heads.
Assay plates are microplate products in whose wells the samples can be analysed using spectroscopic methods. For example, fluorescence, luminescence, UV-VIS absorption or laser measurements are possible. Porvair-Sciences offers suitable assay plates for all these applications, all of which are produced according to ANSI/SLAS specifications.
Cell culture plates are microplates in which the surface is specially treated to improve cell growth. This is particularly suitable for cells that do not grow easily on plastic.
Storage Plates are generally used for the storage of samples in the life science industry. However, other applications in the fields of biopharmacology, biotechnology and especially HPLC-MS also require highly pure and stable microplate products with which reliable and reproducible results can be achieved. Extractables and leachables, i.e. extractable impurities and additives in the well plate material, interfere with the analysis and must be avoided for scientific applications.
Filtration plates can be used in the simplest case to separate solids from liquids, whereby either the solid or the liquid is required for further studies. There are optimised filtration plates for applications such as cell harvesting, DNA separations, binding studies, plasmid isolation, general filtration and sample purification. Glass fibre, PES PVDF, polypropylene or polyethylene are available as filter materials.
Technical Data
Assay plates have been developed for analysis using spectroscopic methods. When analysing, it is possible to measure either from above (top reader) or from below (bottom reader).
Top-reading devices are based on the measurement of the reflected light over the wells:
- white assay plates for adsorption measurements
- black assay plates for fluorescence measurements
Bottom-reading devices irradiate the sample from below and then use detectors placed below the plate to measure the absorbance or fluorescence/luminescence emission. This requires the use of clear bottom plates. The translucent bottom must therefore allow the wavelengths of interest to pass through without interference:
- Measurements 900-350 nm: Polystyrene floors are sufficient
- Measurements 350-220 nm: UV-transparent material required
A UV-transparent material can be, for example, a quartz glass plate or a special polymer such as cyclo-olefin copolymer (COP/COC). For CCD or laser detection measurements as well as for high-resolution microscopy, Porvair Sciences offers assay plates with borosilicate glass bottoms.
Porvair's Krystalâ„¢ series includes assay plates for bottom-reading applications (although some of these can also be used for top-reading applications). Krystalâ„¢ assay plates therefore have transparent bottoms.
Clear Solid Polystyrene Assay Plates
- 96-well plates for routine adsorption, ELISA, mixing and storage applications
- Made from crystalline polystyrene
- Available with different bottom shapes
- Flat for spectroscopic applications
- V-bottom to minimise residual liquid
- U-bottom for holding cells/particles
- Robot compatible
- Working volumes from 275µl up to 10µl
Opaque Solid Polystyrene Assay Plates
- 96-well plates in black, white or black with white wells
- 384-well plates in black, white and clear
- For adsorption, fluorescence, luminescence and scintillation applications
- Standard 'chimney' wells to avoid optical interference and contamination
- The black and white composite plate is up to ten times more sensitive for chemiluminescence assays than pure white plates
Krystalâ„¢ Black & White Assay Plates
- Available as a 24-well, 96-well or 384-well version
- Opaque walls to avoid optical interference
- Clear bottoms allow direct microscopy
- Can be used with both top and bottom reading devices
- 96-well Krystalâ„¢ 2000 optimised for fluorescence and luminescence measurements
Krystalâ„¢ Polystyrene Clear Bottom
- Standard clear polystyrene floor panels
- Ultrasonically welded for perfect sealing in every application
- 0.4mm thick (384-well)
- 0.75mm (24- and 96-well)
Krystalâ„¢ Glass Bottom Plates
- Polystyrene plates with borosilicate glass bases for CCD imaging or laser detection
- Available as 24-well, 96-well or 384-well version
- 175μm glass thickness with a UV cut-off at 335nm
- Very low autofluorescence
- Biocompatible adhesive
- Resistant to alcohol, DMSO and PBS
Krystalâ„¢ UV Clear Bottomed Microplates
- Ideal for laboratories measuring circular dichroism (CD)
- Well plates with UV-transparent bottoms made of a cyclo olefin polymer or quartz glass
- Available as 96-well or 384-well version
- UV cut-off (COP) at 220nm
- Applications e.g. UV fluorescence assays, plate imaging or confocal microscopy
Downloads
Brochures
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Porvair Assay Plates Brochure 2017
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Porvair Catalogue of Microplates & Microplates Equipment
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Porvair Chromatography and Sample Preparation Catalogue 2018
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Porvair Deep Well Microplate for KingFisher Systems Brochure
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Porvair Low Profile Microplates Brochure
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Porvair Sample Preparation SPE and Protein Crash Brochure
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Porvair Plant and Seed Genomics Deep Well Microplates Brochure
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Porvair Ultra Clean Polypropylene Storage Plates Catalogue 2018
Technical support
Porvair Controlling Inherent Contamination in Deep Well Microplates
Porvair Deep-well Plates Selection Guide
Applications
Porvair Plant Genomics Microplate Cannabis Applications
Porvair Biological Sample Preparation and Protein Precipitation in 96 Well Plates Applications
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