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Microlute P3 Protein Crash Plates
Biological samples commonly contain proteins that interfere with downstream applications. While biological sample preparation these proteins often must be removed. The P3 plate uses the "crash" method, in which the protein is denatured with acetronitrile and the flocculant filtered out, allowing 96 samples to be handled at one time.
The Protein Precipitation Plate, P3, is based on the industry standard MicroLute™ format, but without the chromatographic sorbent. Protein ‘crashes’ out of solution and precipitates directly in each well when acetonitrile is added, thus solving all common problems associated with the CRASH technique of protein clean-up.
Product Line
Technical Data
Further advantages of the P3 Plates
- Dual frit design
- Pre-filter frit at 120 μm traps large particles
- Secondary frit traps fine protein particles at <10µm
- Frits are Hydrophobic. This retains sample/acetonitrile in the well to allow precipitation of proteins until vacuum is applied
- Pore size optimised to allow ideal flow rate
- Inert filter material to ensure no adsorption of sample components
- Frit structure prevents break through of protein particles
- Industry standard MicroLute™ format enables easy automation
- Specially selected polypropylene for low extractables
- Disposable reservoir tray. Used for the collection of waste products in the vacuum manifold
- Sealing cap, square well, fits top of MicroLute™. Used for retaining the sample in the well during transit or stopping the well from becoming contaminated
- Drain cap mat that seals the bottom of the plate, preventing liquid leakag
Downloads
Brochures
Porvair Chromatography and Sample Preparation Catalogue 2018
Applications
Porvair Biological Sample Preparation and Protein Precipitation in 96 Well Plates Applications
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