Perkin-Elmer / Brownlee

Brownlee

Brownlee is a brand name for HPLC columns manufactured by Perkin Elmerâ„¢. The phases are based on high-purity, spherical silica gel and are available in many modifications for a wide variety of applications.

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Technical Data

  1. Brownlee Analytical: HPLC phases for routine analysis based on 110 Ã… silica
  2. Brownlee Analytical DB: Fully base-deactivated 140 Ã… silica for analysis of small molecules as well as small peptides
  3. Brownlee Aquapore® und Bio: HPLC phases based on 300 Ã… silica for analysis of biomolecules
  4. Brownlee Choiceâ„¢: HPLC phases based on 60 Ã… silica with high surface area, high carbon loads and high retention
  5. Brownlee Spheri-5® und Spheri-10®: Small-pore (80 Ã…, 5 μm), silica based sorbent for separating small molecules. Both monofunctional and polyfunctional C8 and C18 sorbents available.
  6. Brownlee Validated: High purity HPLC phases with inherent low silanophilic activity. For the analysis of acids, bases and neutral compounds.  
  7. Brownlee SPP: Core-Shell (Fused-Coreâ„¢) phasen for fast and high resolution separations of various compounds. The particle size is 2.7 µm (Core: 1.7 µm).

1. Brownlee Analytical HPLC columns

Phase name

Modification

Particle size

Pore size

End-capping

C-content

USP

Surface area

pH range

Tmax

C18

C18

3, 5 µm

110 Ã…

+

13%

L1

N/A

2.5-7.5

80 °C

C8

C8

5 µm

110 Ã…

+

7%

L7

N/A

2.5-7.5

80 °C

phenyl

phenyl

5 µm

110 Ã…

+

6%

L11

N/A

2.5-7.5

80 °C

Biphenyl

Biphenyl

5 µm

110 Ã…

+

Proprietary

L11

N/A

2.5-7.5

80 °C

Cyano

Cyano (CN)

5 µm

110 Ã…

+

4%

L10

N/A

2.5-7.5

80 °C

Amino

Amino (NH2)

3, 5 µm

110 Ã…

-

2%

L8

N/A

2.5-7.5

80 °C

PAH

C18

4 µm

110 Ã…

+

Proprietary

N/A

N/A

2.5-7.5

80 °C

Silica

-

5 µm

110 Ã…

-

-

L3

N/A

2.5-7.5

80 °C

2. Brownlee Analytical DB (U)HPLC columns

Phase name

Modification

Particle size

Pore size

End-capping

C-content

USP

Surface area

pH range

Tmax

C18

C18

1.9, 3, 5 µm

140 Ã…

+

11%

L1

N/A

2.5-8.0

80 °C

Aq C18

C18

1.9, 3, 5 µm

140 Ã…

Proprietary

6%

L1

N/A

2.5-8.0

80 °C

Biphenyl

Biphenyl

1.9, 3, 5 µm

140 Ã…

+

8%

L11

N/A

2.5-8.0

80 °C

Cyano

Cyano (CN)

1.9, 5 µm

140 Ã…

-

4%

L10

N/A

2.5-8.0

80 °C

PFP Propyl

Pentafluorophenyl

1.9, 3, 5 µm

140 Ã…

+

6%

L43

N/A

2.5-8.0

80 °C

IBD

Polar group embedded Alkyl

1.9 µm

140 Ã…

+

Proprietary

L68

N/A

2.5-8.0

80 °C

PAH

C18

1.9 µm

140 Ã…

+

Proprietary

N/A

N/A

2.5-8.0

80 °C

Silica

-

1.9 µm

140 Ã…

-

-

L3

N/A

2.5-8.0

80 °C

3. Brownlee Aquapore and Bio HPLC columns

Phase name

Modification

Particle size

Pore size

End-capping

C-content

USP

Surface area

pH range

Tmax

AP OD-300 (C18)

C18

7 µm

300 Ã…

+

10%

L1

N/A

2.5-8.0

60 °C

AP RP-300 (C8)

C8

7 µm

300 Ã…

+

5%

L7

N/A

2.5-8.0

60 °C

AP AX-300[1]

Polyetdyleneimine

7 µm

300 Ã…

N/A

N/A

N/A

N/A

2.0-8.0

60 °C

AP CX-300[2]

Polyamide coating with carboxymetdyl groups

7 µm

300 Ã…

N/A

N/A

N/A

N/A

2.0-8.0

60 °C

Bio C18

C18

5 µm

300 Ã…

+

9%

L1

N/A

2.5-8.0

80 °C

Bio C8

C8

5 µm

300 Ã…

+

5%

L7

N/A

2.5-8.0

80 °C

[1] Weak Anion Exchanger [2] Weak Cation Exchanger

4. brownlee Choiceâ„¢ HPLC columns

Phase name

Modification

Particle size

Pore size

End-capping

C-content

USP

Surface area

pH range

Tmax
C18

C18

5 µm

60 Ã…

+

27%

L1

N/A

2.5-8.0

80 °C
Aq C18

C18

5 µm

60 Ã…

-

Proprietary

L1

N/A

2.5-8.0

80 °C
Biphenyl

Biphenyl

5 µm

60 Ã…

-

23%

L11

N/A

2.5-8.0

80 °C
PFP Propyl

Pentafluorophenyl

3, 5 µm

60 Ã…

+

17%

L43

N/A

2.5-8.0

80 °C
Basix

Phenyl Cyano

5 µm

60 Ã…

+

12%

L10

N/A

2.5-8.0

80 °C
Organic Acid[1]

N/A

5 µm

60 Ã…

+

Proprietary

N/A

N/A

2.5-8.0

80 °C
AK[2]

N/A

5 µm

60 Ã…

-

Proprietary

N/A

N/A

2.5-8.0

80 °C

[1 ] Special phase for the separation of organic acids
[2] Special phase for the separation of DNPH derivatised aldehydes and ketones

5. Brownlee Spheri-5 and Spheri-10 HPLC columns

Phase name

Modification

Particle size

Pore size

End-capping

C-content

USP

Surface area

pH range

Tmax

Spheri-5 C18

C18

5 µm

80 Ã…

+

11%

L1

N/A

N/A

N/A

Spheri-5 C18 ODS (Polyfunctional)

C18

5 µm

80 Ã…

+

14%

L1

N/A

N/A

N/A

Spheri-10 C18

C18

10 µm

80 Ã…

+

11%

L1

N/A

N/A

N/A

Spheri-10 C2

C2

10 µm

80 Ã…

-

N/A

L16

N/A

N/A

N/A

Spheri-5 Silica

-

5 µm

80 Ã…

N/A

N/A

L3

N/A

N/A

N/A

6. Brownlee Validated HPLC columns

Phase name

Modification

Particle size

Pore size

End-capping

C-content

USP

Surface area

pH range

Tmax

C18

C18

3, 5 µm

100 Ã…

+

20%

L1

N/A

2.5-8.0

80 °C

Aq C18

C18

3, 5 µm

100 Ã…

-

15%

L1

N/A

2.5-8.0

80 °C

C8

C8

5 µm

100 Ã…

+

12%

L7

N/A

2.5-8.0

80 °C

C4

C4

5 µm

100 Ã…

+

9%

L26

N/A

2.5-8.0

80 °C

C1

C1

5 µm

100 Ã…

-

5%

L13

N/A

2.5-8.0

80 °C

phenyl

phenyl

3, 5 µm

100 Ã…

+

10%

L11

N/A

2.5-8.0

80 °C

Cyano

Cyano (CN)

5 µm

100 Ã…

+

8%

L10

N/A

2.5-8.0

80 °C

Amino

Amino (NH2)

3, 5 µm

100 Ã…

-

2%

L8

N/A

2.5-8.0

80 °C

PFP Propyl

Pentafluorophenyl

5 µm

100 Ã…

+

11%

L43

N/A

2.5-8.0

80 °C

IBD

Polar group embedded Alkyl

3, 5 µm

100 Ã…

-

12%

L68

N/A

2.5-8.0

80 °C

Carbamate[1]

N/A

3, 5 µm

100 Ã…

Proprietary

Proprietary

N/A

N/A

2.5-8.0

80 °C

Quat[2]

N/A

5 µm

100 Ã…

Proprietary

Proprietary

N/A

N/A

2.5-8.0

80 °C

[1] Special phase for the analysis of diquat, paraquat and other quaternary amines
[2] Special phase for the analysis of carbamates

7. Brownlee SPP (U)HPLC columns

Phase name

Modification

Particle size

Pore size

End-capping

C-content

USP

Surface area

pH range

Tmax

C18

C18

2.7 µm

90 Ã…

+

N/A

N/A

N/A

2.0-9.0

60 °C

Peptides ES-C18

C18

2.7 µm

160 Ã…

+

N/A

N/A

N/A

1.0-8.0

90 °C

C8

C8

2.7 µm

90 Ã…

+

N/A

N/A

N/A

2.0-9.0

60 °C

Phenylhexyl

Phenylhexyl

2.7 µm

90 Ã…

+

N/A

N/A

N/A

2.0-9.0

60 °C

PFP

Pentafluorophenyl

2.7 µm

90 Ã…

+

N/A

N/A

N/A

2.0-8.0

60 °C

RP amides

Amide embedded Alkyl

2.7 µm

90 Ã…

+

N/A

N/A

N/A

2.0-9.0

60 °C

HILIC

N/A

2.7 µm

90 Ã…

-

N/A

N/A

N/A

2.0-8.0

60 °C

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