Miles Scientific / Reversed Phase & Modified UNIPLATES

Reversed Phase & Modified UNIPLATES

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Reversed Phase & Modified UNIPLATES

Reversed Phase Separation (RPS) UNIPLATES consist of a hydrocarbon impregnated silica gel. The hydrocarbon is adsorbed onto the silica gel and is not chemically bonded to the particles. The main advantage with this method is the compatibility with aqueous solutions (up to 100%). The disadvantage is that these plates do not tolerate non-polar solvents. Ethyl acetate or less polar solvents will wash the hydrocarbon from the silica particles.

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Technical Data

Sodium Hydroxide (0.1N) modified Silica Gel G & GF UNIPLATES are manufactured with sodium hydroxide instead of water in the preparation process. This makes the plates more basic and leads to an improved separation of certain compounds (e.g., organometallics and some acidic classes).

Carbomer (0.33%) modified Silica Gel H UNIPLATES are special plates prepared according to pharmacopoeia methods for the analysis of mannitol/sorbitol. Caution: These plates are very fragile and are mostly shipped with silica flakes detach from the plate. These plates are not returnable!

Silver Nitrate impregnated Silica Gel UNIPLATES are impregnated between 5 and 20%. This results in an increased discrimination of certain compounds, particularly those containing carbon-carbon double bonds. The concentration of silver nitrate can be specified by the customer.

Magnesium Acetate modified Silica Gel H & HF UNIPLATES are prepared with 5, 7.5 or 10% magnesium acetate in the slurry liquid. This decreases the acidity of the plate and allows increased discrimination of phospholipids

Ammonium Sulfate modified Silica Gel H & HF UNIPLATES are especially made for charring visualization without the need of spraying sulfuric acid on the plate. The plates must be dried at 150-200 °C for 30 to 60 minutes to achieve charring.

Potassium Oxalate modified Silica Gel H & HF UNIPLATES are prepared with 1% potassium oxalate to aid the discrimination of polyphosphoinositides. The “F” indicates the suitability for UV detection at 254nm for all plates.

Ordering information RPS UNIPLATES

(5 % carbon loading, 25 plates/box, 15 µm particle size, 60 Å pore size)

Plate size	Thickness	Scored	Art. No. RPS	Art. No. RPSF
20 x 20 cm	250 µm	-	50011	52011
20 x 20 cm	250 µm	+	50511	52511
10 x 20 cm	250 µm	-	50021	52021
10 x 20 cm	250 µm	+	50521	52521
5 x 20 cm	250 µm	-	50031	52031
20 x 20 cm	500 µm	-	50012	52012
20 x 20 cm	500 µm	+	50512	52512
10 x 20 cm	500 µm	-	50022	52022
5 x 20 cm	500 µm	-	50032	52032
20 x 20 cm	1000 µm	-	50013	52013

Order Information Carbomer (0.33%) modified Silica Gel H UNIPLATES

(25 plates/box, 750 µm thickness, plate size 20 x 20 cm, 19 µm particle size, 60 Å pore size) P/N 70019

Ordering information Sodium hydroxide (0.1N) modified silica gel G & GF UNIPLATES

(25 plates/box, 250 µm thickness, 10 µm particle size, 60 Å pore size)

Plate size	Art. No. G	Art. No. GF
20 x 20 cm	68011	69011
10 x 20 cm	68021	69021
5 x 20 cm	68031	69031

Ordering information Silver nitrate impregnated silica gel UNIPLATES

(25 plates/box, 10 µm particle size, 60 Å pore size)

Plate size	Thickness	Art. No. without F	Art. No. with F
20 x 20 cm	250 µm	28011	29011
10 x 20 cm	250 µm	28021	29021
5 x 20 cm	250 µm	28031	29031
20 x 20 cm	500 µm	28012	29012
20 x 20 cm	1000 µm	28013	29013

Ordering information Magnesium acetate modified silica gel H & HF UNIPLATES

(25 plates/box, 19 µm particle size, 60 Å pore size)

Plate size	Thickness	Art. No. H	Art. No. HF
20 x 20 cm	250 µm	88011	89011
10 x 20 cm	250 µm	88021	89021
5 x 20 cm	250 µm	88031	89031
20 x 20 cm	500 µm	88012	89012

Ordering information Ammonium sulphate modified silica gel H & HF UNIPLATES

(25 plates/box, 250 µm thickness, 19 µm particle size, 60 Å pore size)

Plate size	Art. No. H	Art. No. HF
20 x 20 cm	74011	75011
10 x 20 cm	74021	75021
5 x 20 cm	74031	75031

Ordering information Potassium oxalate modified silica gel H & HF UNIPLATES

(25 plates/box, 250 µm thickness, 19 µm particle size, 60 Å pore size)

Plate size	Art. No. H	Art. No. HF
20 x 20 cm	86011	87011
10 x 20 cm	86021	87021
5 x 20 cm	86031	87031

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