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Discovery
Discovery® is a brand name for various reversed-phase HPLC columns from Supelco™ (Sigma Aldrich™). Base material is a spherical, high-purity silica, which is available in various modifications. Unique combinations of polar and hydrophobic retention mechanisms provide differentiated separations compared to octadecyl phases.
The Discovery® Zr Series includes HPLC columns with zirconia-based stationary phases. Discovery® Zr comprises four phase chemistries bonded to porous, spherical zirconia particles. Zirconia particles have exceptional pH and thermal stability compared to silica and alumina particles. Compared to polymer particles, zirconia does not shrink or swell with changes in temperature, ionic strength, or organic concentration, and has exceptional mechanical strength. Lewis acid - Lewis base interactions as an additional retention mechanism provides unique selectivity properties.
The Discovery® Bio Series includes several wide-pore phases for the analysis of peptides and proteins.
Technical Data
| Phase-Name | Modification | Particle Size | Pore Size | End-capping | C-Content | USP | Surface | pH-Range | Tmax |
| Discovery® HS C18 | C18 | 3, 5, 10 µm | 120 Å | + | 20% | L1 | 300 m2/g | 2.0‒8.0 | 70 °C |
| Discovery® C18 | C18 | 5 µm | 180 Å | + | 12% | L1 | 200 m2/g | 2.0‒8.0 | 70 °C |
| Discovery® C8 | C8 | 5 µm | 180 Å | + | 7.5% | L7 | 200 m2/g | 2.0‒8.0 | 70 °C |
| Discovery® Cyano | Cyano | 5 µm | 180 Å | + | 4.5% | L10 | 200 m2/g | 2.0‒8.0 | 70 °C |
| Discovery® HS F5 | Pentafluorophenylpropyl | 3, 5, 10 µm | 120 Å | + | 12% | L43 | 300 m2/g | 2.0‒8.0 | 70 °C |
| Discovery® RP-AmideC16 | Palmitamidopropyl | 5 µm | 180 Å | + | 11% | N/A | 200 m2/g | 2.0‒8.0 | 70 °C |
| Discovery® HS PEG | Polyetdylene glycol | 3, 5, 10 µm | 120 Å | N/A | N/A | N/A | 300 m2/g | N/A | N/A |
Discovery-Zr Series
| Phase-Name | Modification | Particle Size | Pore Size | End-capping | C-Content | USP | Surface | pH-Range | Tmax |
| Discovery® Zr-PBD | Polybutadiene | 3, 5 µm | 300 Å | - | 2% | L49 | 30 m2/g | 1.0-13.0 | 100 °C[1] |
| Discovery® Zr-PS | Polystyrene | 3, 5 µm | 300 Å | - | 2% | N/A | 30 m2/g | 1.0-13.0 | 100 °C[1] |
| Discovery® Zr-Carbon C18 | C18-phenyl modified carbon | 3, 5 µm | 300 Å | - | 3% | N/A | 30 m2/g | 1.0-14.0 | 100 °C[2] |
| Discovery® Zr-Carbon | Graphitic like Carbon | 3, 5 µm | 300 Å | - | 1% | N/A | 30 m2/g | 1.0-14.0 | 100 °C[2] |
| Discovery® Zr-SAX | Polyetdyleneimine | 3 µm | 300 Å | - | 25% | N/A | 30 m2/g | 1.0-12.0 | 80 °C |
[1] Special hardware for operation between 100 °C and 150 °C is available
[2] Special hardware for operation between 100 °C and 200 °C is available
Discovery-Bio Series
| Phase-Name | Modification | Particle Size | Pore Size | End-capping | C-Content | USP | Surface | pH-Range | Tmax |
| Discovery® BIO Wide Pore C18 | Polybutadiene | C18 | 3, 5, 10 µm | + | 9% | L1 | 100 m2/g | 2.0‒8.0 | 70 °C |
| Discovery® BIO Wide Pore C8 | Polystyrene | C8 | 3, 5, 10 µm | + | 5% | L7 | 100 m2/g | 2.0‒8.0 | 70 °C |
| Discovery® BIO Wide Pore C5 | C18-phenyl modified carbon | C8 | 33, 5, 10 µm | + | 3.5% | N/A | 100 m2/g | 2.0‒8.0 | 70 °C |
Downloads
Brochures
Merck Supelco Discovery BIO Brochure
Merck Supelco Discovery BIO Wide Pore HPLC Columns Catalogue 2019
Merck Supelco Discovery HS PEG Brochure
Applications
Merck Supelco Applications-Notebook Vol1 HPLC, UHPLC, LC-MS, SFC, SPE
Merck Supelco Discovery HS F5 Analysis of Acrylamide in Fried Potato Chips Applications
Merck Supelco Discovery HS F5 Analysis of Acrylamide and Acrylic Acid Applications
Technical Support
Merck Supelco Discovery Method Development Guide
Merck Supelco Discovery Zr Method Development Guide
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