Merck Supelco (Sigma-Aldrich) / BIOshell

BIOshell

BIOshell columns from Merck Supelco consist of core-shell particles for bioseparations ranging from glycans to large proteins. These columns are compatible with HPLC and UHPLC systems as well as for MS detection.

BIOshell IgG 1000 Å: for mAbs, ADCs, biosimilars, H/D exchange, mAb fragments

BIOshell A400 Protein: mAbs, ADCs, biosimolars, Proteins, mAb fragments

BIOshell A160 Peptide: for tryptic digest, post-translational modifications, polypeptides

BIOshell Glycan: for oligosaccharides via HILIC

The new BIOshell A120 Oligo columns are also based on fused-core technology. A special surface modification allows the columns to be used at higher pH values and temperatures. This and the larger pores of 120Å make these columns ideal for analysing oligonucleotides.

20% Discount on Discovery

Product Line

Products

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BIOshell A160 Peptide PCS-C18, 2.7 µm HPLC Column, L × I.D. 100 mm × 4.6 mm

SU-50665-U

€729.00

Product Type: CoreShell Column Length: 100 mm Internal Diameter: 4.6 mm Particle Size: 2.7 µm Pore Size: 160 Å Chemistry: C18 (RP-18, ODS, Octadecyl) Manufacturer: Merck Supelco Delivery Time: approx. 3 days Column Type: Analytical Column Carbon Load: 5.1 % Bet Surface Area [m2/g]: 75.0 Mode of Separation: Reversed Phase Chromatography (RP) Hardware Type: Ready-to-use Column Matrix: Silica Particle Morphology: spherical, solid core, porous shell pH Minimum: 2.0 pH Maximum: 7.0 Pressure Stability: 600 bar Technology: HPLC

BIOshell A160 Peptide PCS-C18, 2.7 µm HPLC Column, L × I.D. 50 mm × 4.6 mm

SU-50664-U

€650.00

Product Type: CoreShell Column Length: 50 mm Internal Diameter: 4.6 mm Particle Size: 2.7 µm Pore Size: 160 Å Chemistry: C18 (RP-18, ODS, Octadecyl) Manufacturer: Merck Supelco Delivery Time: approx. 3 days Column Type: Analytical Column Carbon Load: 5.1 % Bet Surface Area [m2/g]: 75.0 Mode of Separation: Reversed Phase Chromatography (RP) Hardware Type: Ready-to-use Column Matrix: Silica Particle Morphology: spherical, solid core, porous shell pH Minimum: 2.0 pH Maximum: 7.0 Pressure Stability: 600 bar Technology: HPLC

BIOshell A160 Peptide PCS-C18, 2.7 µm HPLC Column, L × I.D. 150 mm × 3.0 mm

SU-50663-U

€795.00

Product Type: CoreShell Column Length: 150 mm Internal Diameter: 3.0 mm Particle Size: 2.7 µm Pore Size: 160 Å Chemistry: C18 (RP-18, ODS, Octadecyl) Manufacturer: Merck Supelco Delivery Time: approx. 3 days Column Type: Analytical Column Carbon Load: 5.1 % Bet Surface Area [m2/g]: 75.0 Mode of Separation: Reversed Phase Chromatography (RP) Hardware Type: Ready-to-use Column Matrix: Silica Particle Morphology: spherical, solid core, porous shell pH Minimum: 2.0 pH Maximum: 7.0 Pressure Stability: 600 bar Technology: HPLC

BIOshell A160 Peptide PCS-C18, 2.7 µm HPLC Column, L × I.D. 100 mm × 3.0 mm

SU-50661-U

€729.00

Product Type: CoreShell Column Length: 100 mm Internal Diameter: 3.0 mm Particle Size: 2.7 µm Pore Size: 160 Å Chemistry: C18 (RP-18, ODS, Octadecyl) Manufacturer: Merck Supelco Delivery Time: approx. 3 days Column Type: Analytical Column Carbon Load: 5.1 % Bet Surface Area [m2/g]: 75.0 Mode of Separation: Reversed Phase Chromatography (RP) Hardware Type: Ready-to-use Column Matrix: Silica Particle Morphology: spherical, solid core, porous shell pH Minimum: 2.0 pH Maximum: 7.0 Pressure Stability: 600 bar Technology: HPLC

BIOshell A160 Peptide PCS-C18, 2.7 µm HPLC Column, L × I.D. 50 mm × 3.0 mm

SU-50660-U

€650.00

Product Type: CoreShell Column Length: 50 mm Internal Diameter: 3.0 mm Particle Size: 2.7 µm Pore Size: 160 Å Chemistry: C18 (RP-18, ODS, Octadecyl) Manufacturer: Merck Supelco Delivery Time: approx. 3 days Column Type: Analytical Column Carbon Load: 5.1 % Bet Surface Area [m2/g]: 75.0 Mode of Separation: Reversed Phase Chromatography (RP) Hardware Type: Ready-to-use Column Matrix: Silica Particle Morphology: spherical, solid core, porous shell pH Minimum: 2.0 pH Maximum: 7.0 Pressure Stability: 600 bar Technology: HPLC

BIOshell A160 Peptide PCS-C18, 2.7 µm HPLC Column, L × I.D. 150 mm × 2.1 mm

SU-50659-U

€795.00

Product Type: CoreShell Column Length: 150 mm Internal Diameter: 2.1 mm Particle Size: 2.7 µm Pore Size: 160 Å Chemistry: C18 (RP-18, ODS, Octadecyl) Manufacturer: Merck Supelco Delivery Time: approx. 3 days Column Type: Analytical Column Carbon Load: 5.1 % Bet Surface Area [m2/g]: 75.0 Mode of Separation: Reversed Phase Chromatography (RP) Hardware Type: Ready-to-use Column Matrix: Silica Particle Morphology: spherical, solid core, porous shell pH Minimum: 2.0 pH Maximum: 7.0 Pressure Stability: 600 bar Technology: HPLC

BIOshell A160 Peptide PCS-C18, 2.7 µm HPLC Column, L × I.D. 100 mm × 2.1 mm

SU-50657-U

€809.00

Product Type: CoreShell Column Length: 100 mm Internal Diameter: 2.1 mm Particle Size: 2.7 µm Pore Size: 160 Å Chemistry: C18 (RP-18, ODS, Octadecyl) Manufacturer: Merck Supelco Delivery Time: approx. 3 days Column Type: Analytical Column Carbon Load: 5.1 % Bet Surface Area [m2/g]: 75.0 Mode of Separation: Reversed Phase Chromatography (RP) Hardware Type: Ready-to-use Column Matrix: Silica Particle Morphology: spherical, solid core, porous shell pH Minimum: 2.0 pH Maximum: 7.0 Pressure Stability: 600 bar Technology: HPLC

BIOshell A160 Peptide PCS-C18, 2.7 µm HPLC Column, L × I.D. 50 mm × 2.1 mm

SU-50656-U

€650.00

Product Type: CoreShell Column Length: 50 mm Internal Diameter: 2.1 mm Particle Size: 2.7 µm Pore Size: 160 Å Chemistry: C18 (RP-18, ODS, Octadecyl) Manufacturer: Merck Supelco Delivery Time: approx. 3 days Column Type: Analytical Column Carbon Load: 5.1 % Bet Surface Area [m2/g]: 75.0 Mode of Separation: Reversed Phase Chromatography (RP) Hardware Type: Ready-to-use Column Matrix: Silica Particle Morphology: spherical, solid core, porous shell pH Minimum: 2.0 pH Maximum: 7.0 Pressure Stability: 600 bar Technology: HPLC

BIOshell A160 Peptide PCS-C18, 2.7 µm HPLC Column, L × I.D. 150 mm × 1.5 mm

SU-50655-U

€816.00

Product Type: CoreShell Column Length: 150 mm Internal Diameter: 1.5 mm Particle Size: 2.7 µm Pore Size: 160 Å Chemistry: C18 (RP-18, ODS, Octadecyl) Manufacturer: Merck Supelco Delivery Time: approx. 3 days Column Type: Capillary Column Carbon Load: 5.1 % Bet Surface Area [m2/g]: 75.0 Mode of Separation: Reversed Phase Chromatography (RP) Hardware Type: Ready-to-use Column Matrix: Silica Particle Morphology: spherical, solid core, porous shell pH Minimum: 2.0 pH Maximum: 7.0 Pressure Stability: 600 bar Technology: HPLC

BIOshell A160 Peptide PCS-C18, 2.7 µm HPLC Column, L × I.D. 100 mm × 1.5 mm

SU-50654-U

€745.00

Product Type: CoreShell Column Length: 100 mm Internal Diameter: 1.5 mm Particle Size: 2.7 µm Pore Size: 160 Å Chemistry: C18 (RP-18, ODS, Octadecyl) Manufacturer: Merck Supelco Delivery Time: approx. 3 days Column Type: Capillary Column Carbon Load: 5.1 % Bet Surface Area [m2/g]: 75.0 Mode of Separation: Reversed Phase Chromatography (RP) Hardware Type: Ready-to-use Column Matrix: Silica Particle Morphology: spherical, solid core, porous shell pH Minimum: 2.0 pH Maximum: 7.0 Pressure Stability: 600 bar Technology: HPLC

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Technical Data

BIOshell IgG 1000 Å

Phase	Modification	Particle size	Pore size	USP	Tmax
BIOshell IgG 1000 Å C4	C4	2.7 μm	1000 Å	L26	90 °C
BIOshell IgG 1000 Å C18	C18	2.7 μm	1000 Å	L1	90 °C
BIOshell IgG 1000 Å Diphenyl	Diphenyl	2.7 μm	1000 Å	L11	90 °C

BIOshell A400 Protein

Phase	Modification	Particle size	Pore size	USP	Tmax
BIOshell A400 Protein C4	C4	3.4 μm	400 Å	L26	90 °C
BIOshell A400 Protein C18	C18	3.4 μm	400 Å	L1	90 °C

BIOshell A160 Peptide

Phase	Modification	Particle size	Pore size	USP	Tmax	pH range
BIOshell A160 Peptide Phenyl-Hexyl	Hexyl-Ph	2.7 μm	160 Å	L11	90 °C	1.0-8.0
BIOshell A160 Peptide C18	C18	2, 2.7, 5 μm	160 Å	L1	90 °C	1.0-8.0
BIOshell A160 Peptide Cyano	CN	2.7, 5 μm	160 Å	L10	90 °C	1.0-8.0

BIOshell Glycan

Phase	Modification	Particle size	Pore size	Carbon content	Surface area	USP	Tmax
BIOshell Glycan	proprietary pentahydroxy ligand	2.7 μm	90 Å	3.2 %C	135 m²/g	L95	65 °C

BIOshell A120 Oligo

Phase

Modification

Particle size

Pore size

Carbon content

Surface area

USP

Tmax

BIOshell Oligo C18

C18, proprietary surface modification

2.7 μm

120 Å

5.6 %C

75 m²/g

85 °C (pH 9)

90 °C (pH 2)

Applications

Effect of Phase Chemistry on Protein Selectivity

Measurement of denosumab with different columns.

Monoclonal antibodies are unique molecules and therefore can interact differently with different phase chemistries.
The numbered peaks correspond to IgG2 disulfide bond variants.

Reverse phase separation of IgG with different modifications of the BIOshell 1000A columns from Merck Supelco

Test Conditions:

Columns: BIOshell™ IgG 1000 Å C4, 15 cm x 2.1 mm I.D., 2.7 μm

BIOshell™ IgG 1000 Å C18, 15 cm x 2.1 mm I.D., 2.7 μm

BIOshell™ IgG 1000 Å Diphenyl, 15 cm x 2.1 mm I.D., 2.7 μm

Mobile Phase A: 2:10:88 n-propanol/acetonitrile/water (0.1% v/v difluoroacetic acid

Mobile Phase B: 70:20:10 n-propanol/acetonitrile/water (0.1% v/v difluoroacetic acid)

Gradient: 16% B to 26% B in 20 min

Flow rate: 0.2 mL/min

Temperature: 80°C

Detection: UV, 280 nm

Injection volume: 2 µL

Sample: Denosumab, 2 mg/mL, water (0.1% v/v trifluoroacetic acid)

Rapid Protein Separations on BIOshell™ A400

Rapid Protein Separations on BIOshell™ A400.

Due to the shallow, porous shell, protein separations can be performed in less than one minute with the BIOshell™ A400 column.

Reverse phase separation of proteins with the BIOshell C4 400A from Merck Supelco

1. lysozyme; chicken 14.3 kDa

2. haptoglobulin, phenotype 1-1; human 96 kDa

3. glutamate dehydrogenase; bovine 332 kDa

4. ß-galactosidase; E. coli 465 kDa

5. lactate dehydrogenase contaminant

6. lactate dehydrogenase; rabbit 140 kDa

Different Phase Chemistries Allows for Alternative Selectivity for Peptides

Measurement of amino acid sequences with different columns.

Better sequence coverage, through better resolution of critical pairs, can be achieved by using phase chemistries orthogonal to C18.

Reverse phase separations of peptides with different functionalizations of BIOshell 160A columns from Merck Supelco

1. FTISADTSKNTAYLQMNSLR

2. LSCAASGFNIKDTYIHWVR

3. GFYPSDIAVEWESNGQPENNYK

4. LLIYSASFLYSGVPSR

5. SGTASVVCLLNNFYPR

6. SCDKTHTCPPCPAPELLGGPSVFLFPPKPK

7. VVSVLTVLHQDWLNGKEYK

Downloads

Brochures

HPLC UHPLC
Merck Supelco BIOshell Brochure

Applications

HPLC
Merck Supelco BIOshell A160 Peptide Applications
HPLC
Merck Supelco BIOshell A400 Protein C4 Applications
HPLC
Merck Supelco BIOshell Glycan Applications
HPLC
Merck Supelco BIOshell IgG 1000 A Applications
HPLC UHPLC SFC SPE
Merck Supelco Applications-Notebook Vol1 HPLC, UHPLC, LC-MS, SFC, SPE

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