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BIOshell
BIOshell columns from Merck Supelco consist of core-shell particles for bioseparations ranging from glycans to large proteins. These columns are compatible with HPLC and UHPLC systems as well as for MS detection.
BIOshell IgG 1000 Å: for mAbs, ADCs, biosimilars, H/D exchange, mAb fragments
BIOshell A400 Protein: mAbs, ADCs, biosimolars, Proteins, mAb fragments
BIOshell A160 Peptide: for tryptic digest, post-translational modifications, polypeptides
BIOshell Glycan: for oligosaccharides via HILIC
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Technical Data
BIOshell IgG 1000 Å
| Phase | Modification | Particle size | Pore size | USP | Tmax |
| BIOshell IgG 1000 Å C4 | C4 | 2.7 μm | 1000 Å | L26 | 90 °C |
| BIOshell IgG 1000 Å C18 | C18 | 2.7 μm | 1000 Å | L1 | 90 °C |
| BIOshell IgG 1000 Å Diphenyl | Diphenyl | 2.7 μm | 1000 Å | L11 | 90 °C |
BIOshell A400 Protein
| Phase | Modification | Particle size | Pore size | USP | Tmax |
| BIOshell A400 Protein C4 | C4 | 3.4 μm | 400 Å | L26 | 90 °C |
| BIOshell A400 Protein C18 | C18 | 3.4 μm | 400 Å | L1 | 90 °C |
BIOshell A160 Peptide
| Phase | Modification | Particle size | Pore size | USP | Tmax | pH range |
| BIOshell A160 Peptide Phenyl-Hexyl | Hexyl-Ph | 2.7 μm | 160 Å | L11 | 90 °C | 1.0-8.0 |
| BIOshell A160 Peptide C18 | C18 | 2, 2.7, 5 μm | 160 Å | L1 | 90 °C | 1.0-8.0 |
| BIOshell A160 Peptide Cyano | CN | 2.7, 5 μm | 160 Å | L10 | 90 °C | 1.0-8.0 |
BIOshell Glycan
| Phase | Modification | Particle size | Pore size | Carbon content | Surface area | USP | Tmax |
| BIOshell Glycan | proprietary pentahydroxy ligand | 2.7 μm | 90 Å | 3.2 %C | 135 m²/g | L95 | 65 °C |
Applications
Effect of Phase Chemistry on Protein Selectivity
Measurement of denosumab with different columns.
Monoclonal antibodies are unique molecules and therefore can interact differently with different phase chemistries.
The numbered peaks correspond to IgG2 disulfide bond variants.
Test Conditions:
Columns: BIOshell™ IgG 1000 Å C4, 15 cm x 2.1 mm I.D., 2.7 μm
BIOshell™ IgG 1000 Å C18, 15 cm x 2.1 mm I.D., 2.7 μm
BIOshell™ IgG 1000 Å Diphenyl, 15 cm x 2.1 mm I.D., 2.7 μm
Mobile Phase A: 2:10:88 n-propanol/acetonitrile/water (0.1% v/v difluoroacetic acid
Mobile Phase B: 70:20:10 n-propanol/acetonitrile/water (0.1% v/v difluoroacetic acid)
Gradient: 16% B to 26% B in 20 min
Flow rate: 0.2 mL/min
Temperature: 80°C
Detection: UV, 280 nm
Injection volume: 2 µL
Sample: Denosumab, 2 mg/mL, water (0.1% v/v trifluoroacetic acid)
Rapid Protein Separations on BIOshell™ A400
Rapid Protein Separations on BIOshell™ A400.
Due to the shallow, porous shell, protein separations can be performed in less than one minute with the BIOshell™ A400 column.
1. lysozyme; chicken 14.3 kDa
2. haptoglobulin, phenotype 1-1; human 96 kDa
3. glutamate dehydrogenase; bovine 332 kDa
4. ß-galactosidase; E. coli 465 kDa
5. lactate dehydrogenase contaminant
6. lactate dehydrogenase; rabbit 140 kDa
Different Phase Chemistries Allows for Alternative Selectivity for Peptides
Measurement of amino acid sequences with different columns.
Better sequence coverage, through better resolution of critical pairs, can be achieved by using phase chemistries orthogonal to C18.
1. FTISADTSKNTAYLQMNSLR
2. LSCAASGFNIKDTYIHWVR
3. GFYPSDIAVEWESNGQPENNYK
4. LLIYSASFLYSGVPSR
5. SGTASVVCLLNNFYPR
6. SCDKTHTCPPCPAPELLGGPSVFLFPPKPK
7. VVSVLTVLHQDWLNGKEYK
Downloads
Brochures
Merck Supelco BIOshell Brochure
Applications
Merck Supelco BIOshell A160 Peptide Applications
Merck Supelco BIOshell A400 Protein C4 Applications
Merck Supelco BIOshell Glycan Applications
Merck Supelco BIOshell IgG 1000 A Applications
Merck Supelco Applications-Notebook Vol1 HPLC, UHPLC, LC-MS, SFC, SPE
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