Ludger / LudgerSep

LudgerSep

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LudgerSep

LudgerSep products are a range of specialised HPLC and UHPLC columns and associated buffer solutions for the precise separation and analysis of glycans. These products are optimised for various chromatographic techniques:

LudgerSep N Amide columns contain particles with a polymeric amide coating that are optimised for high-resolution chromatography of complex glycan mixtures. They are suitable for the analysis and purification of glycans labelled with fluorophores or UV chromophores.

LudgerSep R Reverse columns are equipped with octadecylsilane-coated particles that have been optimised for hydrophobic chromatography. They are mainly used for the analysis of monosaccharides labelled with 2-aminobenzoic acid (2-AA).

LudgerSep C Anion columns have macroporous anion exchange particles and are designed for charge-based analysis and purification of glycans. They are particularly useful for determining the charge profile of glycans labelled with 2-AB or 2-AA.

Product Line

LudgerSep

Technical Data

Phase	Modification	Matrix	Particle size	Pore size	Application	pH range	Temperature
LudgerSep N1	Amide	N/A	5 µm	80 Å	Analysis of 2-AA and 2-AB labelled N- and O-glycans based on hydrophilicity	2.0 - 7.5	10 - 80 °C
LudgerSep N2	amide	N/A	3 µm	80 Å	Analysis of 2-AA and 2-AB labelled N- and O-glycans based on hydrophilicity	2.0 - 7.5	10 - 40 °C
LudgerSep R1	C18	Silica	3 µm	120 Å	Analysis of sialic acid labelled with DMB	2.0 - 8.0	15 - 50 °C
LudgerSep R2	C18	silica	3 µm	175 Å	Analysis of monosaccharides labelled with 2-AA	2.0 - 8.0	15 - 50 °C
LudgerSep UR2 100x2.1mm	C18	silica	1.9 µm	175 Å	Analysis of sialic acid labelled with DMB	1.0 - 11.0	15 - 60 °C
LudgerSep UR2 50x2.1mm	C18	silica	1.9 µm	175 Å	Analysis of monosaccharides labelled with 2-AA	1.0 - 11.0	15 - 60 °C
LudgerSep C2	Polyethyleneimine	Styrene-divinylbenzene	8 µm	N/A	Charge profile of 2-AB and 2-AA labelled glycans	1.0 - 13.0	10 - 60 °C
LudgerSep C3	N/A	Hydrophilic polymer	10 µm	1000 Å	Charge profile of 2-AB and 2-AA labelled glycans	2.0 - 12.0	10 - 45 °C

2-AA: 2-aminobenzoic acid

2-AB: 2-aminobenzamide

DMB: (1-2-diamino-4,5-methylenedioxybenzene)

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