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LudgerSep
LudgerSep products are a range of specialised HPLC and UHPLC columns and associated buffer solutions for the precise separation and analysis of glycans. These products are optimised for various chromatographic techniques:
LudgerSep N Amide columns contain particles with a polymeric amide coating that are optimised for high-resolution chromatography of complex glycan mixtures. They are suitable for the analysis and purification of glycans labelled with fluorophores or UV chromophores.
LudgerSep R Reverse columns are equipped with octadecylsilane-coated particles that have been optimised for hydrophobic chromatography. They are mainly used for the analysis of monosaccharides labelled with 2-aminobenzoic acid (2-AA).
LudgerSep C Anion columns have macroporous anion exchange particles and are designed for charge-based analysis and purification of glycans. They are particularly useful for determining the charge profile of glycans labelled with 2-AB or 2-AA.
Product Line
Technical Data
| Phase | Modification | Matrix | Particle size | Pore size | Application | pH range | Temperature |
| LudgerSep N1 | Amide | N/A | 5 µm | 80 Å | Analysis of 2-AA and 2-AB labelled N- and O-glycans based on hydrophilicity | 2.0 - 7.5 | 10 - 80 °C |
| LudgerSep N2 | amide | N/A | 3 µm | 80 Å | Analysis of 2-AA and 2-AB labelled N- and O-glycans based on hydrophilicity | 2.0 - 7.5 | 10 - 40 °C |
| LudgerSep R1 | C18 | Silica | 3 µm | 120 Å | Analysis of sialic acid labelled with DMB | 2.0 - 8.0 | 15 - 50 °C |
| LudgerSep R2 | C18 | silica | 3 µm | 175 Å | Analysis of monosaccharides labelled with 2-AA | 2.0 - 8.0 | 15 - 50 °C |
| LudgerSep UR2 100x2.1mm | C18 | silica | 1.9 µm | 175 Å | Analysis of sialic acid labelled with DMB | 1.0 - 11.0 | 15 - 60 °C |
| LudgerSep UR2 50x2.1mm | C18 | silica | 1.9 µm | 175 Å | Analysis of monosaccharides labelled with 2-AA | 1.0 - 11.0 | 15 - 60 °C |
| LudgerSep C2 | Polyethyleneimine | Styrene-divinylbenzene | 8 µm | N/A | Charge profile of 2-AB and 2-AA labelled glycans | 1.0 - 13.0 | 10 - 60 °C |
| LudgerSep C3 | N/A | Hydrophilic polymer | 10 µm | 1000 Å | Charge profile of 2-AB and 2-AA labelled glycans | 2.0 - 12.0 | 10 - 45 °C |
2-AA: 2-aminobenzoic acid
2-AB: 2-aminobenzamide
DMB: (1-2-diamino-4,5-methylenedioxybenzene)
Downloads
Brochures
Ludger Product Catalogue Brochure
Technical support
Ludger Buffers for WAX and HPLC Analysis of Glycans Technical Support
Ludger How well is your column performing Technical Support
Ludger HPLC and UHPLC Columns Technical Support
Ludger Products Flowchart Technical Support
Applications
Ludger Alpha-Gal -containing Biologics Applications
Ludger Glucose Homopolymer (GHP) - System Suitability and Reference Standard for Glycan Analysis Applications
Ludger O-Glycosilation Analysis Application
Ludger Quantitative Sialic Acid Analysis Application
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