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Helix Chromatography / Amaze
Amaze
Amaze columns are mixed-mode columns. These mixed-mode columns are able to separate a wide range of analytes in one run.
Technical Data
| Phase | Modes | Particle size / μm | Pore size / Å |
| Amaze SC | Reversed phase
| 3, 5, 10 | 100 |
| Amaze TR | Reversal phase
| 3, 5, 10 | 100 |
| Amaze QR | Reversal phase
| 3, 5, 10 | 100 |
| Amaze td | HILIC
| 3, 5, 10 | 100 |
| Amaze HD | Cation exchange
| 2, 3, 5, 10 | 100 |
| Amaze HA | Anion exchange
| 3, 5, 10 | 100 |
| Amaze PFP | π-π interaction
| 3, 5, 10 | 100 |
| Amaze AR | π-π-π interaction
| 3, 5, 10 | 100 |
| Amaze SPF | π-π-π interaction | 3, 5, 10 | 100 |
Amaze SC
- substituted alkyl chains with several polar embedded groups (reverse phase and cation exchange)
- acidic fragments on the ligand interact strongly with basic analytes
- compatible with 100% water and 100% acetonitrile
Amaze TR
- C18 chains with acidic and basic groups on the surface (reverse phase, cation and anion exchange)
- large capacity for neutral, acidic and basic components
- compatible with 100% water and 100% organic mobile phases
Amaze QR
- hydrophobic chains, aromatic fragments, acidic and basic groups (reverse phase, π-π interaction, cation and anion exchange)
- Can separate components that are only slightly different
Amaze TH
- HILILC, cation and anion exchange
- Separates polar neutral, polar acidic, polar basic, hydrophobic acidic and hydrophobic basic components in one run
- Strength of the acidic and basic groups can be adjusted via the pH value
Amaze HD
- polymeric ligand with strong hydrogen donor and strong acidic groups (cation exchange and hydrogen bonding)
- can be used in normal phase, HILIC or cation exchange mode, depending on the mobile phase
- alternative selectivity compared to traditional reversed phase and HILIC columns
Amaze HA
- polymeric ligand with strong hydrogen acceptor and strong basic groups (anion exchange and hydrogen bonding)
- can be used in normal phase, HILIC or anion exchange mode, depending on the mobile phase
- alternative selectivity compared to traditional reversed phase and HILIC columns
Amaze PFP
- long alkyl chains with a polyfluorinated aromatic ring at the end and several polar embedded groups (Ï€-Ï€ interaction and dipole-dipole interaction)
- Separates structural isomers from aromatic compounds
Amaze AR
- long alkyl chains with an aromatic ring at the end and several polar embedded groups (π-ππ interaction and dipole-dipole interaction)
- Separates structural isomers from aromatic compounds
Amaze SPF
- long alkyl chains with two polyflurinated aromatic rings as a "sandwich" ligand and multiple polar embedded groups
- unique selectivity compared to single ring columns
- Separation based on specific π-π interactions
Amaze MH
- trimodal HILIC mixed-mode phase with HILIC, cation and anion exchange properties (azide group pka4, basic groups pka 11)
- for the separation of a variety of different compounds (polar metabolites, amino acids, organic and inorganic acids, basic and azide drugs, environmental pollutants, sugars and structural isomers)
Amaze RP A1/RP A2
- hydrophobic chain with polar embedding group and azide groups near the surface (pka 1/pka 2)
- Separation takes place via reversed phase, cation exchange and anion exclusion
- separates amino acids, hydrophilic neutral and hydrophobic acidic compounds, inorganic cations, hydrophilic basic compounds, basic drugs, hydrophobic anionic counterions and structural isomers
Amaze RP A3
- hydrophobic chain with polar embedding group and azide groups near the surface (pka 3)
- Separation occurs via reversed phase, cation exchange, anion exclusion and chelation
- due to a slightly different ligand, weak chelating effects can also be observed, which is favourable for the separation of primary amines and inorganic cations
Amaze WB
- hydrophobic chain with weakly basic polar embedded groups
- Reversed phase, weak anion exchange and cation exclusion
- Separation of hydrophobic drugs and acidic counterions, hydrophobic neutral and hydrophobic weakly acidic compounds, basic polymers, strong inorganic and organic acids
Amaze IB
- hydrophobic chain with medium to weak basic polar groups (pka 9)
- Reversed phase, medium anion exchange and cation exclusion
- Separation of hydrophobic drugs and acidic counterions, hydrophobic neutral and hydrophobic weakly acidic compounds, basic polymers, strong and weak inorganic and organic acids
Amaze RP SA
- hydrophobic chain with acidic embedded groups downstream of the surface (pka 0)
- Separation takes place via reversed phase, cation exchange and anion exclusion
- Separates amino acids, hydrophilic neutral and hydrophobic acidic compounds, inorganic cations, hydrophilic basic compounds, basic drugs, hydrophobic anionic counterions and structural isomers
Amaze RP AB
- hydrophobic chain with basic and acidic groups on the surface (pka 1.5 and pka 10)
- Reversed phase, cation and anion exchange
- basic and acidic drugs and corresponding counterions, organic and inorganic cations and anions
Downloads
Applications
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Helix Amaze HD Analysis of 18 Amino Acids
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Helix Amaze SC Analysis of 20 Amino Acids on Mixed-Mode Column
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Helix Amaze HA Analysis of Adenosine Diphosphate and Adenosine Triphosphate
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Helix Amaze HD Analysis of Adenosine and Adenine
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Helix Amaze HD Analysis of Nucleosides and Nucleoside Derivatives
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Helix Amaze TCH Separation of Nucleobases and Nucleosides
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