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...these are the latest columns of Advanced Chromatography Technologies Ltd. that have been developed to enable chromatographers to achieve reproducible and successful Hydrophilic Interaction Chromatography, short HILIC, separations using a systematic approach to method development. The columns are available with three unique modifications:
ACE HILIC-A • Acidic character phase (Proprietary SIL)
ACE HILIC-B • Basic character phase (Proprietary Aminopropyl)
ACE HILIC-N • Neutral character phase (Proprietary Polyhydroxy)
Each phase offering alternative selectivity, which allows for a simplified and structured approach to achieving successful HILIC separations. Additionally ACE HILIC Method Development kits are also available. They consist of three ACE HILIC columns, HILIC-A, HILIC-B and HILIC-N, respectively. ACT has created a very useful document for method development support the „ACE Knowledge Note #0021“. A very easy and quick protocol for the method development using the ACE HILIC columns is described in the Knowledge Note.
The columns are available in nearly all common analytical column lengths. Available column IDs and particle sizes are shown in the following table:
Column type | Inner diameter | Particle size |
Analytical | 2.1 mm, 3.0 mm, 4.6 mm | 1.7 µm, 3 µm, 5 µm |
Microbore | 0.5 mm, 1.0 mm | 3 µm, 5 µm |
Determination of β-blockers using HILIC mode
1. Propanolol
2. Sotalol
3. Acebutolol
4. Salbutamol
5. Atenolol
Test conditions:
Column: ACE 5 HILIC-A 150 x 4.6 mm
Mobile phase: 12 mM ammonium formate pH 4.7 in MeCN/H2O (88:12 v/v)
Flow rate: 1.5 mL/min
Injection volume: 2 μL
Temperature: 25 °C
Detection: UV, 230 nm
Determination of Caffeine and Related Compounds using HILIC Mode
1. Caffeine
2. Theophylline
3. Theobromine
4. Xanthine
5. Hypoxanthine
Test Conditions:
Column: ACE 5 HILIC-N, 150 x 4.6 mm
Mobile Phase A: 10 mM ammonium formate pH 3.0 in MeCN/H2O (96:4 v/v)
Mobile Phase B: 10 mM ammonium formate pH 3.0 in MeCN/H2O (50:50 v/v)
Gradient:
Time (min) | %B |
0 | 0 |
15 | 100 |
Flow Rate: 1.5 mL/min
Injection: 2 μL
Temperature: 15 °C
Detection: UV, 275 nm
Determination of Methylmalonic Acid and Succinic Acid using HILIC Mode
1. Succinic acid
2. Methylmalonic acid
Test Conditions:
Column: ACE 5 HILIC-B, 150 x 4.6 mm
Mobile Phase: 10 mM ammonium formate pH 3.0 in MeCN/H2O (90:10 v/v)
Flow Rate: 1.5 mL/min
Injection: 5 µL
Temperature: 25 °C
Detection: ELSD, Evaporator temperature: 30 °C, Nebuliser temperature: 30 °C
Gas speed: 1 SLM