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Phase name | Particle size | Pore size | End-capping | C-content | Surface area | pH range |
Avantor ACE Excel Glycan | 1.7, 3 µm | 100 Å | - | 7.0 % | 300 m2/g | 2.0-7.0 |
The Avantor ACE Excel glycan columns are available in three different lengths (50, 100, 150 mm) and two different inner diameters (2.1 and 4.6 mm) and are available exclusively from MZ-Analysentechnik GmbH.
Detection of dextran ladder standard in HILIC with fluorescence detection
GU1-G15: 2-aminobenzamide (2-AB) labeled glucose homopolymers with 1-15 glucose monomer units
Test Conditions:
Column: ACE Excel Glycan, 150 x 2.1 mm
Mobile Phase A: 100 mM ammonium formate in water (pH 4.5)
Mobile Phase B: MeCN
Gradient:
Time (min) | %B |
0 | 75 |
24.0 | 60 |
24.3 | 40 |
24.6 | 40 |
24.9 | 75 |
45.0 | 75 |
Flow Injection: 0.5 µL/min
Injectionvolume: 1.5 µL
Detection: FLD, λex = 260 nm, λem = 430 nm
Dwell Volume: 1950 µL
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