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Poroshell
Poroshell® (U)HPLC Columns, manufactured by Agilent Technologies™, are based on Core-Shell (Fused-Core™) Technology. The silica-based stationary phases consist of a non-porous core and an porous outer layer, that provides fast separations without excessive backpressure. Poroshell® columns are available with different modifications for many different applications, even for enantiomer separations. The particle sizes are 1.9 µm (Core: N/A), 2.7 μm (core: 1.7 μm) and 4 μm (core: N/A).
Poroshell® 300 Phases with 300 Å pore size and 5 µm (core: 4.5 µm) particle size are ideally suited for large molecules, e.g. peptides or proteins.
Technical Data
| Phase name | Modification | Particle size | Pore size | End-capping | C-content | USP | Surface area | pH range | Tmax |
| Poroshell® 120 EC-C18 | C18 | 1.9, 2.7, 4 µm | 120 Å | + | 10% | L1 | 130 m2/g | 2.0-8.0 | 60 °C |
| Poroshell® 120 SB-C18 | C18 | 2.7 µm | 120 Å | - | 8% | L1 | 130 m2/g | 1.0-8.0 | 90 °C |
| Poroshell® HPH-C18 | C18 | 1.9, 2.7, 4 µm | 100 Å | + | Proprietary | L1 | 95 m2/g | 3.0-11.0 | 60 °C |
| Poroshell® 120 CS-C18 | C18 | 2.7 µm | 120 Å | + | Proprietary | N/A | 95 m2/g | 2.0-8.0 | 60 °C |
| Poroshell® 120 EC-C8 | C8 | 1.9, 2.7, 4 µm | 120 Å | + | 5% | L7 | 130 m2/g | 2.0-8.0 | 60 °C |
| Poroshell® 120 SB-C8 | C8 | 2.7 µm | 120 Å | - | 5.5% | L7 | 130 m2/g | 1.0-8.0 | 80 °C |
| Poroshell® HPH-C8 | C8 | 2.7, 4 µm | 100 Å | + | Proprietary | L7 | 95 m2/g | 3.0-11.0 | 60 °C |
| Poroshell® 120 Phenyl-Hexyl | Phenyl-Hexyl | 1.9, 2.7, 4 µm | 120 Å | + | 9% | L11 | 130 m2/g | 2.0-8.0 | 60 °C |
| Poroshell® 120 EC-CN | Cyano | 2.7 µm | 120 Å | + | 3.5% | L10 | 130 m2/g | 2.0-8.0 | 60 °C |
| Poroshell® 120 PFP | Pentafluorophenyl | 1.9, 2.7, 4 µm | 120 Å | + | 5.1% | L43 | 130 m2/g | 2.0-8.0 | 60 °C |
| Poroshell® 120 SB-Aq | N/A | 2.7 µm | 120 Å | - | Proprietary | L96 | 130 m2/g | 1.0-8.0 | 80 °C |
| Poroshell® 120 Bonus-RP | Amide embedded Alkyl | 2.7 µm | 120 Å | + | 9.5% | L60 | 130 m2/g | 2.0-8.0 | 60 °C |
| Poroshell® 120 HILIC | - | 1.9, 2.7, 4 µm | 120 Å | - | N/A | L3 | 130 m2/g | 0.0-8.0 | 60 °C |
| Poroshell® 120 HILIC-Z | N/A | 2.7 µm | 120 Å | - | Proprietary | N/A | 130 m2/g | 2.0-12.0 [1] | 80 °C [2] |
| Poroshell® 120 HILIC-OH5 | N/A | 2.7 µm | 120 Å | Proprietary | Proprietary | N/A | 130 m2/g | 1.0-7.0 | 45 °C |
| Poroshell® Chiral-T | Teicoplanin | 2.7 µm | 120 Å | Proprietary | Proprietary | N/A | 130 m2/g | 2.5-7.0 | 45 °C |
| Poroshell® Chiral-V | Vancomycin | 2.7 µm | 120 Å | Proprietary | Proprietary | L88 | 130 m2/g | 2.5-7.0 | 45 °C |
| Poroshell® Chiral-CD | Hydroxypropylated β-cyclodextrin | 2.7 µm | 120 Å | Proprietary | Proprietary | N/A | 130 m2/g | 3.0-7.0 | 45 °C |
| Poroshell® Chiral-CF | Derivatised cyclofructan (CF6) | 2.7 µm | 120 Å | Proprietary | Proprietary | N/A | 130 m2/g | 3.0-7.0 | 45 °C |
[1] at 35 °C [2] at pH=7
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