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Polargel columns from Agilent Technologies™ are designed for size exclusion chromatography with intermediate polarity solvents and polar solvent combinations. The columns exhibit excellent stability and lifetime in challenging polar solvents and at elevated temperatures. PolarGel “mixed bed” columns have a medium polarity surface and high mechanical stability. They are capable of operating in a wide range of solvents and solvent combinations, greatly enhancing their ability to analyze polar polymers that are not soluble in traditional aqueous or organic solvents.