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Mixed Mode Phases
Mixed-mode phases for HPLC utilise different separation techniques in parallel in one column. The stationary phases are specially/differently modified for this purpose so that, for example, reversed phase and ion exchange properties are simultaneously involved in the separation. Nowadays, there are many different mixed-mode phases that combine a wide variety of separation modes. They exhibit unique selectivities, making them the column of choice for some difficult separations.
Technical Data
Manufacturer of mixed-mode columns
| Manufacturer | Phase name | Modification | Separation mechanisms involved | Particle size | Pore size | pH range |
| Imtakt | Scherzo SS-C18 | C18 + SAX + SCX | Reversed phase Anion Exchange Cation Exchange Normal Phase | 3 µm | 130 Å | N/A |
Scherzo SM-C18 | C18 + WAX + WCX | Reversed phase Anion Exchange Cation Exchange Normal Phase | 3 µm | 130 Å | N/A | |
Scherzo SW-C18 | C18 + SAX + SCX | Reversed phase Anion Exchange Cation Exchange Normal Phase | 3 µm | 130 Å | N/A | |
| Shodex | Asahipak GS-HQ[1] | N/A | Size Exclusion (SEC) Reversed Phase HILIC | 6, 7 & 13 µm | 150, 400, | 2.0-9.0 |
| Sepax | HP-SCX | Sulfonic Acid + Phenyl | Cation Exchange Reversed Phase | 1.8, 3, 5 & 10 µm | 120 Å | 1.5-8.0 |
HP-SAX | Quat. Ammonium + Phenyl | Anion Exchange Reversed Phase | 5 µm | 120 Å | 1.5-8.0 | |
| Osaka Soda | Capcell Pak CR | C18 + SCX | Reversed Phase Cation Exchange | 3 & 5 µm | N/A | N/A |
| Thermo Scientific | Acclaim Mixed-Mode HILIC-1 | Alkyl chain with | Reversed-Phase HILIC | 3 & 5 µm | 120 Å | 2.5-7.5 |
Acclaim Mixed-Mode WAX-1 | Alkyl chain with | Reversed Phase Anion Exchange | 3 & 5 µm | 120 Å | 2.5-7.5 | |
Acclaim Mixed-Mode WCX-1 | Alkyl chain with | Reversed Phase Cation Exchange Anion Exclusion | 3 & 5 µm | 120 Å | 2.5-7.5 | |
Acclaim Trinity P1 | RP + SCX + WAX | Reversed Phase Anion Exchange Cation Exchange | 3 µm | 300 Å | 2.5-7.0 | |
Acclaim Trinity P2 | WCX + SAX | HILIC Anion Exchange Cation Exchange | 3 µm | 300 Å | 2.0-8.0 | |
Acclaim Trinity Q1 | RP + WCX + WAX | Reversed Phase Anion Exchange Cation Exchange | 3 µm | 300 Å | 2.5-7.5 | |
| Sielc | Primesep A | RP + embedded SCX | Reversed Phase Cation Exchange | 5 & 10 µm | 100 Å | N/A |
Primesep 100 | RP + embedded CX | Reversed Phase Cation Exchange | 2.7, 3, 5, & 10 µm | 100 Å | N/A | |
Primesep 200 | RP + embedded WCX | Reversed Phase Cation Exchange | 5 & 10 µm | 100 Å | N/A | |
Primesep B | RP + embedded SAX | Reversed Phase Anion Exchange | 5 & 10 µm | 100 Å | N/A | |
Primesep B2 | RP + embedded WAX | Reversed Phase Anion Exchange | 5 & 10 µm | 100 Å | N/A | |
Primesep AB | RP + embedded AX + CX | Reversed-Phase Anion Exchange Cation Exchange | 5 & 10 µm | 100 Å | N/A | |
Primesep C | Proprietary | Reversed Phase Cation Exchange Complex Formation | 5 & 10 µm | 100 Å | N/A |
[1] Base Material: Polyvinylalcohol
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