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Affinity Chromatography
Affinity chromatography is a bioseparation technique that is used for the purification and analysis of proteins, nucleic acids or hormones, among other things. We have compiled a selection of available affinity chromatography columns from market leaders for you. Below you will find information on the base material, ligands and applications for the individual brand columns from Tosoh Bioscience, Shodex, Merck, Separation Methods Technologies (SMT) and Agilent. If you need further details or support, just contact us!
Technical Data
Basics of affinity chromatography
In affinity chromatography, the separation or purification of a substance is based on a highly specific biochemical interaction of the analyte with certain binding partners that are bound to the stationary phase (similar to antigen-antibody or enzyme-inhibitor interaction). Only one sample component in the existing substance mixture is retained by the stationary phase, while the other molecules can be easily rinsed off the column. At the end, the desired sample remains on the column bed, which can then be eluted by displacement, by changing the pH value or by changing the salt concentration.
Figures: Schematic representation of the separation of three proteins using affinity chromatography: Figure 1: Only protein-2 can bind to the ligands of the stationary phase. Proteins-1 and -3 can be removed by washing; Figure 2: After elution of proteins-1 and -3, protein-2 alone remains on the column bed; Figure 3: Protein-2 is eluted under suitable conditions.
Manufacturers and columns for affinity chromatography
The right column for you - we will be happy to support you individually
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Certificates of analysis, MSDS, data sheets