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Fipronil
Fipronil is a pesticide that is used in many countries around the world. It is effective against a large number of agricultural pests, but also has a harmful effect on humans above certain levels. It is suspected of being carcinogenic. The use of fipronil in food-producing animals is prohibited.
Chemically speaking, fipronil belongs to the phenylpyrazole group. It is practically insoluble in water, but shows good solubility in polar organic solvents such as acetone or ethyl acetate. Fipronil is an optically active substance, i.e. fipronil is present in two mutually enantiomeric forms. These can be separated using chiral HPLC. However, fipronil is used as a racemate (as a mixture of enantiomers in a 1:1 ratio).
Fipronil is often analysed using liquid or gas chromatography coupled to a mass spectrometer (LC-MS or GC-MS). The QuEChERS method is often used for sample preparation.
Applications
Chiral separation with Supelco Astec (R,R) P-CAP
Test conditions
Column: Astec (R,R) P-CAP 250x4.6mm, 5µm (31024AST)
Mobile phase: 80/20 heptane/ethanol
Flow rate: 1 mL/min
Temperature: 25 °C
Detection: UV, 235 nm
Sample: 1 mg/mL in methanol
Reversed phase separation with Nouryon Kromasil Eternity C18
Peak identities
1. fipronil
Test conditions
Column: Kromasil Eternity 2.5-C18 50x4.6mm (EH2CLA05)
Mobile phase: 70/30/0.2 acetonitrile/water/acetic acid
Flow rate: 1 mL/min
Temperature: 40 °C
Detection: UV, 260 nm
Gas chromatography with Supelco SLB-5ms
Peak identities
1. triflurlin 2. impurity 3. propyzamide 4. primicarb 5. chlorpiriphos-methyl 6. pyrimiphos-ethyl 7. fipronil 8. Dazomet isomer 9. Dazomet isomer 10. fluazifop-p-butyl 11. fenazaquin 12. benfuracarb 13. impurity 14. azoxystrobin
Test conditions
Column: SLB-5ms 10m x 0.1mm, 0.1 µm (28465-U)
Downloads
Manufacturer overview
Chiral
HPLC - Chiral separation:
- Chiralpak IB, 5µm
GL Sciences
Gas chromatography:
- InertCap Pesticides
Imtakt
HPLC - Reversed Phase:
- Unison UK-C18, 3µm
- Cadenza CD-C18, 3µm
Macherey-Nagel
HPLC - Reversed Phase:
- Nucleoshell Bluebird RP 18, 2.7µm
Sample preparation:
- QuEChERS
Nouryon - Kromasil
HPLC - Reversed Phase:
- Kromasil Eternity C18, 2.5µm
Supelco
HPLC - Chiral separation:
- Astec (R,R) P-CAP, 5µm
Gas chromatography:
- SLB-5ms
Thermo Fisher
HPLC - Reversed Phase:
- Accucore aQ, 2.6µm
UHPLC - Reversed Phase
- Hypersil GOLD aQ, 1.9µm
Gas chromatography:
- TarceGOLD TG-5Sil MS
Sample preparation:
- QuEChERS
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