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Vial Septa
Vial septa are an essential part of chromatography and play a crucial role in sample preparation and analysis. They act as sealing elements between the vial and the cap and ensure that the sample is securely sealed and protected from contamination.
Septa are available in different materials, each offering specific properties and advantages. The most common materials are polytetrafluoroethylene (PTFE), silicone, and a combination of both (PTFE/silicone). PTFE septa are chemically inert and resistant to a wide range of solvents, making them ideal for working with aggressive chemicals. Silicone septa offer excellent elasticity and impermeability, making them particularly suitable for frequent needle punctures. PTFE/silicone septa combine the chemical resistance of PTFE with the flexibility of silicone, making them versatile.
An important aspect of vial septa is their ability to withstand repeated needle punctures without losing their seal. This is particularly important in automated systems where samples are frequently withdrawn and injected. High quality septa prevent sample or solvent leakage and protect the sample from environmental contamination.
Choosing the right septum depends on several factors, including the type of sample, the solvents used and the specific requirements of the analysis. For example, gas chromatography applications may require different septa than liquid chromatography to meet the specific requirements of each method.
Another advantage of high-quality septa is the reduction of ghost signals and background noise in chromatographic data. By using pure and high quality materials, the risk of unwanted interactions and contamination is minimised, leading to clearer and more accurate results.
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