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Quechers
QuEChERS is a sample preparation method developed for the fast and efficient extraction of pesticides and other residues from food samples. It stands for"Quick, Easy, Cheap, Effective, Rugged, and Safe" and describes the main advantages of this technique. QuEChERS simplifies the analysis process by using a combination of acetonitrile extraction and salt purification to ensure high purity of the extracted samples. This method has become widely used in food analysis due to its simplicity, cost-effectiveness and reliability.
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Technical Data
What different methods are there?
This special method for sample preparation was first published in 2003 by M. Anastassiades et al. in the"Journal of AOAC International"[1]. Since then, QuEChERS has become the most widely used method for sample preparation in pesticide analysis worldwide. To date, two methods for QuEChERS analysis have become established and are officially recognised: the European EN 15662 Method[2] and the AOAC Official 2007.01 Method[3]. The main difference between these two QuEChERS methods is the type of buffer salt used. The method according to EN 15662:2008[2] uses a citrate buffer, the method according to AOAC 2007. 01[3] uses an acetate buffer. The original published method does not require buffer salts[1].
What is the general procedure for QuEChERS analysis?
The procedure of this method is quite simple and the susceptibility to errors is very low. In addition, QuEChERS shows high recovery rates and can be applied to many analytes to be analysed.
First, the crude sample is mixed with a water-soluble solvent (e.g. acetonitrile) and an internal standard in a centrifuge tube and shaken well. Buffer salts are then added for better phase separation and stabilisation of acid- and base-labile pesticides. After thorough shaking and centrifugation, the crude extract is obtained. After the phases have separated, part of the upper phase is removed and further purified using dispersive solid phase extraction. Dispersive because, in contrast to normal solid phase extraction, the solid sorbent is now added directly to the sample. It is then shaken thoroughly and centrifuged again. This is how the final extract is obtained. The supernatant can then be analysed using liquid or gas chromatography in conjunction with other analytical methods (e.g. mass spectroscopy).
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